scholarly journals Characterization of Na+-K+-2Cl−Cotransporter Activity in Rabbit Lacrimal Gland Duct Cells

2016 ◽  
Vol 57 (8) ◽  
pp. 3828 ◽  
Author(s):  
Eszter Vizvári ◽  
Máté Katona ◽  
Péter Orvos ◽  
Orsolya Berczeli ◽  
Andrea Facskó ◽  
...  
Keyword(s):  
Lacrimal Gland ◽  
Duct Cells ◽  
Gland Duct

Analysis of Gene Expression in Rat Lacrimal Gland Duct Cells using Laser Capture Microdissection and cDNA Microarrays

2005 ◽  
Vol 3 ◽  
pp. S123
Author(s):  
John L. Ubels ◽  
Holly M. Hoffman ◽  
Sujata Srikanth ◽  
James H. Resau ◽  
Craig P. Webb
Keyword(s):  
Gene Expression ◽  
Lacrimal Gland ◽  
Laser Capture Microdissection ◽  
Cdna Microarrays ◽  
Duct Cells ◽  
Gland Duct ◽  
Laser Capture

scholarly journals Patch-Clamp Recording of K+ Current from Lacrimal Gland Duct Cells

2019 ◽  
Author(s):  
Loren D. Haarsma ◽  
John L. Ubels
Keyword(s):  
Patch Clamp ◽  
Lacrimal Gland ◽  
Acinar Cells ◽  
Sprague Dawley ◽  
Patch Clamp Recording ◽  
Pipette Solution ◽  
Relative Paucity ◽  
Duct Cells ◽  
Gland Duct ◽  
Electrophysiologic Studies

AbstractElectrophysiologic studies have characterized ion channels in lacrimal gland acinar cells, but due to relative paucity and inaccessibility, such studies on lacrimal gland duct cells are challenging. The duct cells are believed to secrete the high level of K+ that is present in tears. The goal of this project was to develop a method for isolation of viable, single duct cells, demonstrate their utility for patch-clamp recording and characterize the K+ channels expressed by duct cells. Exorbital lacrimal gland slices from Sprague-Dawley rats were incubated with collagenase. Under a microscope, the ducts were microdissected from the glands and then incubated with elastase and collagenase. Dispersed duct cells were plated on a cover slip coated with BD Cell-Tak. Duct cells were distinguished from acinar cells by their smaller size and lack of granularity. Whole-cell K+ currents were recorded from duct cells using the perforated-patch technique and pipettes with resistances of less than 10 MΩ. EGTA and CaCl2 in the pipette solution were adjusted to give 1 uM free Ca++. When held at −80 mV, duct cells showed K+ currents that activated at command voltages near 0 mV and reached amplitudes near 1 nA at +100 mV. Currents reached peak amplitude less than 20 ms after depolarization and did not inactivate. These currents were inactivated by holding the cells at 0 mV. Currents were blocked reversibly by TEA in the presence of Ca2+, but were not blocked by TEA in the absence of Ca2+. Currents were not affected by clotrimazole (10 uM) or Ba2+ (5 mM). A method has been established for isolation and dissociation lacrimal gland duct cells for electrophysiologic studies. These cells express a voltage-activated K+ channel that is dependent on the presence of intracellular Ca2+ and may correspond to the IKCa1 channel expressed on the apical membranes of lacrimal gland ducts.

Cell culture and characterization of human minor salivary gland duct cells

1989 ◽  
Vol 18 (4) ◽  
pp. 214-219 ◽  
Author(s):  
Barbara E. Kurth ◽  
Debra J. Hazen-Martin ◽  
Mary Ann Sens ◽  
Richard W. DeChamplain ◽  
Donald A. Sens
Keyword(s):  
Cell Culture ◽  
Salivary Gland ◽  
Minor Salivary Gland ◽  
Duct Cells ◽  
Gland Duct

scholarly journals Saliva and its potential in covid-19 cannot be ignored: a point of view

2021 ◽  
pp. 1-5
Author(s):  
Sukhvinder Singh Oberoi ◽  
Shabina Sachdeva ◽  
Shibani Grover ◽  
Vivek Sharma
Keyword(s):  
Salivary Gland ◽  
Epithelial Cells ◽  
Human Body ◽  
Point Of View ◽  
High Potential ◽  
Healthy Human ◽  
Duct Cells ◽  
Gland Duct

Abstract It has been demonstrated that salivary gland duct cells have similar receptors as ACE2-positive cells/keratin epithelial cells of the lung which have high potential to be infected by SARS-CoV 2 virus. The aerosols carrying virus have penetration into the healthy human body and lungs via inhalation through nose or mouth.

scholarly journals Characterization of P2X7Purinergic Receptors and Their Function in Rat Lacrimal Gland

2009 ◽  
Vol 50 (12) ◽  
pp. 5681 ◽  
Author(s):  
Robin R. Hodges ◽  
Joanna Vrouvlianis ◽  
Marie A. Shatos ◽  
Darlene A. Dartt
Keyword(s):  
Lacrimal Gland

scholarly journals Reversible Conversion among Subtypes of Salivary Gland Duct Cells as Identified by Production of a Variety of Bioactive Polypeptides

2019 ◽  
Vol 52 (4) ◽  
pp. 59-65
Author(s):  
Shingo Kurabuchi ◽  
Chenjuan Yao ◽  
Gang Chen ◽  
Kazuo Hosoi
Keyword(s):  
Salivary Gland ◽  
Duct Cells ◽  
Gland Duct

THE HISTOCHEMISTRY AND CYTOLOGY OF THE SALIVARY GLAND DUCT CELLS

1954 ◽  
Vol 20 (2) ◽  
pp. 116-128 ◽  
Author(s):  
Ruth Hill ◽  
G.H. Bourne
Keyword(s):  
Salivary Gland ◽  
Duct Cells ◽  
Gland Duct

scholarly journals Experimental Evidence of Fluid Secretion of Rabbit Lacrimal Gland Duct Epithelium

2014 ◽  
Vol 55 (7) ◽  
pp. 4360 ◽  
Author(s):  
Máté Katona ◽  
Eszter Vizvári ◽  
Lukács Németh ◽  
Andrea Facskó ◽  
Viktória Venglovecz ◽  
...  
Keyword(s):  
Experimental Evidence ◽  
Lacrimal Gland ◽  
Fluid Secretion ◽  
Duct Epithelium ◽  
Gland Duct
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