scholarly journals Effects of Insulin and High Glucose on Human Meibomian Gland Epithelial Cells

2015 ◽  
Vol 56 (13) ◽  
pp. 7814 ◽  
Author(s):  
Juan Ding ◽  
Yang Liu ◽  
David A. Sullivan
Keyword(s):  
Epithelial Cells ◽  
High Glucose ◽  
Meibomian Gland

Abstract P415: ACE N-domain Regulates High-glucose Mediated Interleukin-1 beta Production by Renal Epithelial Cells Independently From Angiotensin II Generation

Hypertension ◽  
2017 ◽  
Vol 70 (suppl_1) ◽  
Author(s):  
Jorge F Giani ◽  
Ellen A Bernstein ◽  
Masahiro Eriguchi ◽  
Romer A Gonzalez-Villalobos ◽  
Kenneth E Bernstein
Keyword(s):  
Diabetic Nephropathy ◽  
Angiotensin Ii ◽  
Epithelial Cells ◽  
High Glucose ◽  
Interleukin 1 ◽  
Kidney Injury ◽  
Fold Increase ◽  
Renal Epithelial Cells ◽  
Proximal Tubular ◽  
Sense And Respond

Research studies demonstrated that interleukin (IL)-1β contributes to the development of diabetic nephropathy and hypertension. However, the origin and regulation of IL-1β synthesis during diabetic kidney injury are still unknown. Here, we hypothesize that renal epithelial cells produce IL-1β in response to a high glucose stress and that angiotensin converting enzyme (ACE) plays a key role in this process. To study this, we isolated proximal tubular (PT) epithelial cells from wild-type (WT) and mice lacking either the ACE N-domain (NKO) or the C-domain (CKO) catalytic activity. These cells were exposed to normal (5 mM) or high (30 mM) glucose for 24 hours. IL-1β produced by PT cells were assessed by ELISA and RT-PCR. High glucose induced WT PT cells to release significant amounts of IL-1β (from 5±1 to 70±6 pg/ml, p<0.001; n=6). When WT PT cells were exposed to a high glucose media in the presence of an ACE inhibitor (lisinopril, 10 mM), IL-1β levels were significantly reduced (from 70±6 to 38±6 pg/ml, p<0.01). In contrast, AT1 receptor blockade by losartan did not change the amount of IL-1β produced by WT PT cells. To determine which ACE domain is associated with IL-1β production, NKO and CKO PT cells were exposed to high glucose. Strikingly, NKO PT cells released lower amounts of IL-1β when exposed to high glucose compared to WT (NKO: 15±7 vs. WT: 79±9 pg/ml, p<0.01, n=4). No differences were observed between WT and CKO PT cells. Since the ACE N-domain degrades the anti-inflammatory tetrapeptide N-acetyl-Ser-Asp-Lys-Pro (AcSDKP), we tested whether the lower IL-1β production in NKO PT cells was due to an accumulation of AcSDKP. For this, we pre-treated NKO PT cells with a prolyl endopeptidase inhibitor (S17092, 50μM) to prevent the production of AcSDKP. Notably, this treatment increased the IL-1β response to high glucose in NKO PT cells (2.1±0.3-fold increase, p<0.01, n=4). Our data indicate that: 1) PT cells can sense and respond to high glucose by secreting IL-1β and 2) the absence of the ACE N-domain blunts the production of IL-1β through a mechanism that involves AcSDKP accumulation. In conclusion, ACE might contribute to the inflammatory response that underlays diabetic nephropathy independently from angiotensin II generation.

Prostaglandin E2 and F2α Alter Expression of Select Cholesteryl Esters and Triacylglycerols Produced by Human Meibomian Gland Epithelial Cells

Cornea ◽  
2021 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Jillian F. Ziemanski ◽  
Landon Wilson ◽  
Stephen Barnes ◽  
Kelly K. Nichols
Keyword(s):  
Epithelial Cells ◽  
Prostaglandin E2 ◽  
Meibomian Gland ◽  
Cholesteryl Esters

scholarly journals Serum-Induced Differentiation of Human Meibomian Gland Epithelial Cells

2014 ◽  
Vol 55 (6) ◽  
pp. 3866 ◽  
Author(s):  
David A. Sullivan ◽  
Yang Liu ◽  
Wendy R. Kam ◽  
Juan Ding ◽  
Karin M. Green ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Meibomian Gland ◽  
Induced Differentiation

scholarly journals High glucose modifies heparansulphate synthesis by mouse glomerular epithelial cells

1999 ◽  
Vol 15 (1) ◽  
pp. 13-20 ◽  
Author(s):  
Susanna Morano ◽  
Leo Guidobaldi ◽  
Rosalba Cipriani ◽  
Annarita Gabriele ◽  
Federico Pantellini ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
High Glucose ◽  
Glomerular Epithelial Cells
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