Effects of Mesenchymal Stem/Stromal Cells on Cultures of Corneal Epithelial Progenitor Cells With Ethanol Injury

2014 ◽  
Vol 55 (11) ◽  
pp. 7628 ◽  
Author(s):  
Joo Youn Oh ◽  
Jung Hwa Ko ◽  
Mee Kum Kim ◽  
Won Ryang Wee
Keyword(s):  
Progenitor Cells ◽  
Stromal Cells ◽  
Corneal Epithelial ◽  
Epithelial Progenitor Cells ◽  
Ethanol Injury

scholarly journals Nerve growth factor and its receptor TrkA serve as potential markers for human corneal epithelial progenitor cells

2008 ◽  
Vol 86 (1) ◽  
pp. 34-40 ◽  
Author(s):  
Hong Qi ◽  
De-Quan Li ◽  
H. David Shine ◽  
Zhuo Chen ◽  
Kyung-Chul Yoon ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Progenitor Cells ◽  
Nerve Growth ◽  
Corneal Epithelial ◽  
Epithelial Progenitor Cells

scholarly journals Improved transduction of human corneal epithelial progenitor cells with cell-targeting adenoviral vectors

2006 ◽  
Vol 83 (4) ◽  
pp. 798-806 ◽  
Author(s):  
Zhuo Chen ◽  
Hoyin Mok ◽  
Stephen C. Pflugfelder ◽  
De-Quan Li ◽  
Michael A. Barry
Keyword(s):  
Progenitor Cells ◽  
Adenoviral Vectors ◽  
Cell Targeting ◽  
Corneal Epithelial ◽  
Epithelial Progenitor Cells

scholarly journals Importin 13 Serves as a Potential Marker for Corneal Epithelial Progenitor Cells

Stem Cells ◽  
2009 ◽  
Vol 27 (10) ◽  
pp. 2516-2526 ◽  
Author(s):  
Hua Wang ◽  
Tao Tao ◽  
Jing Tang ◽  
Yi-Hui Mao ◽  
Wei Li ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Corneal Epithelial ◽  
Potential Marker ◽  
Epithelial Progenitor Cells

scholarly journals Evaluation of epithelial progenitor cells and growth factors in a preclinical model of wound healing induced by mesenchymal stromal cells

2020 ◽  
Vol 40 (7) ◽  
Author(s):  
Giselle Ramos-Gonzalez ◽  
Olga Wittig ◽  
Dylana Diaz-Solano ◽  
Lianeth Salazar ◽  
Carlos Ayala-Grosso ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Growth Factor ◽  
Progenitor Cells ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Skin Wound ◽  
Preclinical Model ◽  
Human Mscs ◽  
Epithelial Progenitor Cells

Abstract Background: Skin wounds continue to be a global health problem. Several cellular therapy protocols have been used to improve and accelerate skin wound healing. Here, we evaluated the effect of transplantation of mesenchymal stromal cells (MSC) on the wound re-epithelialization process and its possible relationship with the presence of epithelial progenitor cells (EPC) and the expression of growth factors. Methods: An experimental wound model was developed in C57BL/6 mice. Human MSCs seeded on collagen membranes (CM) were implanted on wounds. As controls, animals with wounds without treatment or treated with CM were established. Histological and immunohistochemical (IH) studies were performed at day 3 post-treatment to detect early skin wound changes associated with the presence of EPC expressing Lgr6 and CD34 markers and the expression of keratinocyte growth factor (KGF) and basic fibroblast growth factor (bFGF). Results: MSC transplantation enhanced skin wound re-epithelialization, as compared with controls. It was associated with an increase in Lgr6+ and CD34+ cells and the expression of KGF and bFGF in the wound bed. Conclusion: Our results show that cutaneous wound healing induced by MSC is associated with an increase in EPC and growth factors. These preclinical results support the possible clinical use of MSC to treat cutaneous wounds.

003. ENDOMETRIUM: CINDERELLA TISSUE IN A STEM CELL WORLD

2009 ◽  
Vol 21 (9) ◽  
pp. 3
Author(s):  
C. E. Gargett
Keyword(s):  
Stem Cells ◽  
Endometrial Cancer ◽  
Stem Cell ◽  
Epithelial Cells ◽  
Progenitor Cells ◽  
Stromal Cells ◽  
Human Endometrium ◽  
Cancer Tissue ◽  
Stem Cell Markers ◽  
Epithelial Progenitor Cells

Despite human endometrium undergoing more than 400 cycles of regeneration, differentiation and shedding during a woman's reproductive years, and that in non-menstruating species (eg rodents) there are cycles of endometrial growth and apoptosis, endometrial stem/progenitor cells have only recently been identified. Since there are no specific stem cell markers, initial studies using functional approaches identified candidate epithelial and stromal endometrial stem/progenitor cells as colony forming cells/units (CFU) (1). Further evaluation of key stem cell properties of individual CFU demonstrated that rare EpCAM+ epithelial cells and EpCAM- stromal cells underwent self renewal by serial subcloning >3 times and underwent >30 population doublings in culture. Clonally-derived epithelial cells differentiated into cytokeratin+ gland-like structures. Single stromal cells were multipotent as they differentiated into 4 mesodermal lineages; myogenic, adipogenic, osteoblastic and chondrogenic, suggesting that human endometrium contains a rare population of epithelial progenitor cells and mesenchymal stem cells (MSC) (2). Transplantation of freshly isolated human endometrial cells into immunocompromised mice reconstructed endometrial tissue that responded to estrogen and progesterone (3). Endometrial MSC can be prospectively isolated by co-expression of CD146 and PDGFRβ (4), but not Stro-1, a bone marrow MSC marker (5). Currently there are no known markers of endometrial epithelial progenitor cells. Endometrial cancer tissue harbours a small subpopulation of clonogenic, self-renewing, tumour-initiating cells, producing tumours that recapitulate parent tumours in histoarchitecture and differentiation markers (ERα, PR, cytokeratin, vimentin) when xenografted into mice, suggesting they are cancer stem cells. Candidate epithelial and stromal stem/progenitor cells have been identified in mouse endometrium as label retaining cells (LRC) in the luminal epithelium and perivascular cells at the endometrial-myometrial junction, respectively (6). It is likely that endometrial stem/progenitor cells play key roles in the development of gynecological diseases associated with abnormal endometrial proliferation such as endometriosis and endometrial cancer (7).

IN VIVO GENE TRANSFER INTO CORNEAL EPITHELIAL PROGENITOR CELLS BY VIRAL VECTORS.

Cornea ◽  
2000 ◽  
Vol 19 (Supplement 2) ◽  
pp. S96
Author(s):  
Tsutomu Igarashi ◽  
Koichi Miyake ◽  
Noriko Suzuki ◽  
Hiroshi Takahashi ◽  
Takashi Shimada
Keyword(s):  
Gene Transfer ◽  
Progenitor Cells ◽  
Viral Vectors ◽  
Corneal Epithelial ◽  
Epithelial Progenitor Cells ◽  
In Vivo Gene Transfer

New strategy for in vivo transgene expression in corneal epithelial progenitor cells

2002 ◽  
Vol 24 (1) ◽  
pp. 46-50 ◽  
Author(s):  
Tsutomu Igarashi ◽  
Koichi Miyake ◽  
Noriko Suzuki ◽  
Ko Kato ◽  
Hiroshi Takahashi ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Transgene Expression ◽  
Corneal Epithelial ◽  
New Strategy ◽  
Epithelial Progenitor Cells
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