Effect of Intravitreal Injection of Bevacizumab on Optic Nerve Head Leakage and Retinal Ganglion Cell Survival in a Mouse Model of Optic Nerve Crush

2013 ◽  
Vol 54 (13) ◽  
pp. 8160 ◽  
Author(s):  
Daniel Rappoport ◽  
Dana Morzaev ◽  
Shirel Weiss ◽  
Mark Vieyra ◽  
James D. Nicholson ◽  
...  
Keyword(s):  
Optic Nerve ◽  
Mouse Model ◽  
Ganglion Cell ◽  
Cell Survival ◽  
Intravitreal Injection ◽  
Optic Nerve Head ◽  
Optic Nerve Crush ◽  
Retinal Ganglion ◽  
Nerve Crush ◽  
Retinal Ganglion Cell Survival

scholarly journals Intravitreal Injection of Liposomes Loaded with a Histone Deacetylase Inhibitor Promotes Retinal Ganglion Cell Survival in a Mouse Model of Optic Nerve Crush

2020 ◽  
Vol 21 (23) ◽  
pp. 9297
Author(s):  
Mi Sun Sung ◽  
Myeong Ju Moon ◽  
Reju George Thomas ◽  
So Young Kim ◽  
Jun Sung Lee ◽  
...  
Keyword(s):  
Optic Nerve ◽  
Mouse Model ◽  
Ganglion Cell ◽  
Intravitreal Injection ◽  
Histone Deacetylase ◽  
Histone Deacetylase Inhibitor ◽  
Optic Nerve Crush ◽  
Retinal Ganglion ◽  
Nerve Crush ◽  
Deacetylase Inhibitor

Various neuroprotective agents have been studied for the treatment of retinal ganglion cell (RGC) diseases, but issues concerning the side effects of systemically administered drugs and the short retention time of intravitreally injected drugs limit their clinical applications. The current study aimed to evaluate the neuroprotective effects of intravitreally injected trichostatin A (TSA)-loaded liposomes in a mouse model of optic nerve crush (ONC) and determine whether TSA-loaded liposomes have therapeutic potential in RGC diseases. The histone deacetylase inhibitor, TSA, was incorporated into polyethylene glycolylated liposomes. C57BL/6J mice were treated with an intravitreal injection of TSA-loaded liposomes and liposomes loaded with a lipophilic fluorescent dye for tracking, immediately after ONC injury. The expression of macroglial and microglial cell markers (glial fibrillary acidic protein and ionized calcium binding adaptor molecule-1), RGC survival, and apoptosis were assessed. We found that the liposomes reached the inner retina. Their fluorescence was detected for up to 10 days after the intravitreal injection, with peak intensity at 3 days postinjection. Intravitreally administered TSA-loaded liposomes significantly decreased reactive gliosis and RGC apoptosis and increased RGC survival in a mouse model of ONC. Our results suggest that TSA-loaded liposomes may help in the treatment of various RGC diseases.

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