Comparison of Swollen and Dextran Deswollen Organ-Cultured Corneas for Descemet Membrane Dissection Preparation: Histological and Ultrastructural Findings

2013 ◽  
Vol 54 (13) ◽  
pp. 8036 ◽  
Author(s):  
Efdal Yoeruek ◽  
Johanna Hofmann ◽  
Karl-Ulrich Bartz-Schmidt
Keyword(s):  
Descemet Membrane ◽  
Membrane Dissection ◽  
Ultrastructural Findings

scholarly journals Ultrastructural findings in graft failure after Descemet membrane endothelial keratoplasty (DMEK) and new triple procedure

Medicine ◽  
2019 ◽  
Vol 98 (19) ◽  
pp. e15493 ◽  
Author(s):  
Isabell Schmidt ◽  
Ursula Schlötzer-Schrehardt ◽  
Achim Langenbucher ◽  
Timo Eppig ◽  
Tobias Hager ◽  
...  
Keyword(s):  
Graft Failure ◽  
Endothelial Keratoplasty ◽  
Descemet Membrane Endothelial Keratoplasty ◽  
Descemet Membrane ◽  
Triple Procedure ◽  
Ultrastructural Findings

Novel Surgical Instruments Facilitating Descemet Membrane Dissection

Cornea ◽  
2013 ◽  
Vol 32 (4) ◽  
pp. 523-526 ◽  
Author(s):  
Efdal Yoeruek ◽  
Karl-Ulrich Bartz-Schmidt
Keyword(s):  
Surgical Instruments ◽  
Descemet Membrane ◽  
Membrane Dissection

Bronchial stereocilia in the immotile cilia syndrome: A case report

1984 ◽  
Vol 42 ◽  
pp. 52-53
Author(s):  
George Price ◽  
Lizardo Cerezo
Keyword(s):  
Surface Modifications ◽  
Respiratory Epithelium ◽  
Immotile Cilia ◽  
Kartagener's Syndrome ◽  
Membrane Blebs ◽  
Radial Spokes ◽  
Males And Females ◽  
Dynein Arms ◽  
Ultrastructural Finding ◽  
Ultrastructural Findings

Ultrastructural defects of ciliary structure have been known to cause recurrent sino-respiratory infection concurrent with Kartagener's syndrome. (1,2,3) These defects are also known to cause infertility in both males and females. (4) Overall, the defects are defined as the Immotile, or Dyskinetic Cilia Syndrome (DCS). Several ultrastructural findings have been described, including decreased number of cilia, multidirection orientation, fused and compound cilia, membrane blebs, excess matrix in the axoneme, missing outer tubular doublets, translocated doublets, defective radial spokes and dynein arms. A rare but noteworthy ultrastructural finding in DCS is the predominance of microvilli-like structures on the luminal surface of the respiratory epithelium. (5,6) These permanent surface modifications of the apical respiratory epithelium no longer resemble cilia but reflect the ultrastructure of stereocilia, similar to that found in the epidydimal epithelium. Like microvilli, stereocilia are devoid of microtubular ultrastructure in comparison with true cilia.

New ultrastructural findings about Borrelia burgdorferi by cryofixation, negative staining, thin sectioning and scanning techniques

1990 ◽  
Vol 48 (3) ◽  
pp. 582-583
Author(s):  
S. F. Hayes ◽  
M. D. Corwin ◽  
T. G. Schwan ◽  
D. W. Dorward ◽  
W. Burgdorfer
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Structural Characterization ◽  
Negative Staining ◽  
Low Speed ◽  
Thin Sectioning ◽  
Ultrastructural Findings

Characterization of Borrelia burgdorferi strains by means of negative staining EM has become an integral part of many studies related to the biology of the Lyme disease organism. However, relying solely upon negative staining to compare new isolates with prototype B31 or other borreliae is often unsatisfactory. To obtain more satisfactory results, we have relied upon a correlative approach encompassing a variety EM techniques, i.e., scanning for topographical features and cryotomy, negative staining and thin sectioning to provide a more complete structural characterization of B. burgdorferi.For characterization, isolates of B. burgdorferi were cultured in BSK II media from which they were removed by low speed centrifugation. The sedimented borrelia were carefully resuspended in stabilizing buffer so as to preserve their features for scanning and negative staining. Alternatively, others were prepared for conventional thin sectioning and for cryotomy using modified procedures. For thin sectioning, the fixative described by Ito, et al.

CYSTADENOMA OF THE SEMINAL VESICLE: REPORT OF A CASE WITH ULTRASTRUCTURAL FINDINGS

Pathology ◽  
2001 ◽  
Vol 33 (3) ◽  
pp. 399-402 ◽  
Author(s):  
Leonardo D. Santos ◽  
Cyril S. K. C. Wong ◽  
Murray Killingsworth
Keyword(s):  
Seminal Vesicle ◽  
Ultrastructural Findings

scholarly journals Descemet membrane suturing to manage recurrent graft detachment in a patient with Descemet membrane endothelial keratoplasty on failed penetrating keratoplasty

2021 ◽  
Vol 13 ◽  
pp. 251584142110277
Author(s):  
Zahra Ashena ◽  
Thomas Hickman-Casey ◽  
Mayank A. Nanavaty
Keyword(s):  
Visual Acuity ◽  
Penetrating Keratoplasty ◽  
Anterior Segment ◽  
Endothelial Keratoplasty ◽  
Descemet Membrane Endothelial Keratoplasty ◽  
Descemet Membrane ◽  
Ocular Coherence Tomography ◽  
Corrected Visual Acuity ◽  
Intracameral Injection ◽  
History Of

A 65-year-old patient with history of keratoconus, mild cataract and penetrating keratoplasty over 30 years ago developed corneal oedema subsequent of graft failure with best corrected visual acuity (BCVA) of counting fingers. He underwent a successful cataract surgery combined with a 7.25 mm Descemet’s Membrane Endothelial Keratoplasty (DMEK) with Sodium Hexafluoride (SF6) gas. His cornea remained oedematous inferiorly at 4 weeks, despite two subsequent re-bubbling due to persistent DMEK detachment inferiorly. This was managed by three radial full thickness 10-0 nylon sutures placed in the inferior cornea along with intracameral injection of air. Following this, his anterior segment ocular coherence tomography (OCT) confirmed complete attachment of the graft, and the sutures were removed 4 weeks later. Unaided visual acuity was 20/63 and BCVA was 20/32 after 8 months. DMEK suturing can be helpful in persistent DMEK detachments, which is refractory to repeated re-bubbling due to uneven posterior surface of previous PK.

scholarly journals Risk Factors for Cystoid Macular Edema After Descemet Membrane Endothelial Keratoplasty

Cornea ◽  
2019 ◽  
Vol 38 (7) ◽  
pp. 820-824 ◽  
Author(s):  
Satoru Inoda ◽  
Takahiko Hayashi ◽  
Hidenori Takahashi ◽  
Itaru Oyakawa ◽  
Hideaki Yokogawa ◽  
...  
Keyword(s):  
Risk Factors ◽  
Macular Edema ◽  
Cystoid Macular Edema ◽  
Endothelial Keratoplasty ◽  
Descemet Membrane Endothelial Keratoplasty ◽  
Descemet Membrane
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