Tear Proteomic Analysis of Patients With Type 2 Diabetes and Dry Eye Syndrome by Two-Dimensional Nano-Liquid Chromatography Coupled With Tandem Mass Spectrometry

2014 ◽  
Vol 55 (1) ◽  
pp. 177 ◽  
Author(s):  
Bing Li ◽  
Minjie Sheng ◽  
Liqi Xie ◽  
Feng Liu ◽  
Guoquan Yan ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Type 2 Diabetes ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Dry Eye ◽  
Proteomic Analysis ◽  
Tandem Mass ◽  
Two Dimensional ◽  
Nano Liquid Chromatography

scholarly journals L(+) and D(-) Lactate Are Increased in Plasma and Urine Samples of Type 2 Diabetes as Measured by a Simultaneous Quantification of L(+) and D(-) Lactate by Reversed-Phase Liquid Chromatography Tandem Mass Spectrometry

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Jean L. J. M. Scheijen ◽  
Nordin M. J. Hanssen ◽  
Marjo P. H. van de Waarenburg ◽  
Daisy M. A. E. Jonkers ◽  
Coen D. A. Stehouwer ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Type 2 Diabetes ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Reversed Phase ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Urinary Levels ◽  
Liquid Chromatography Tandem Mass

Background. Plasma and urinary levels of D-lactate have been linked to the presence of diabetes. Previously developed techniques have shown several limitations to further evaluate D-lactate as a biomarker for this condition.Methods. D- and L-lactate were quantified using ultraperformance liquid chromatography tandem mass spectrometry with labelled internal standard. Samples were derivatized with diacetyl-L-tartaric anhydride and separated on a C18-reversed phase column. D- and L-lactate were analysed in plasma and urine of controls, patients with inflammatory bowel disease (IBD), and patients with type 2 diabetes (T2DM).Results. Quantitative analysis of D- and L-lactate was achieved successfully. Calibration curves were linear (r2>0.99) over the physiological and pathophysiological ranges. Recoveries for urine and plasma were between 96% and 113%. Inter- and intra-assay variations were between 2% and 9%. The limits of detection of D-lactate and L-lactate in plasma were 0.7 μmol/L and 0.2 μmol/L, respectively. The limits of detection of D-lactate and L-lactate in urine were 8.1 nmol/mmol creatinine and 4.4 nmol/mmol creatinine, respectively. Plasma and urinary levels of D- and L-lactate were increased in patients with IBD and T2DM as compared with controls.Conclusion. The presented method proved to be suitable for the quantification of D- and L-lactate and opens the possibility to explore the use of D-lactate as a biomarker.

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