scholarly journals Imaging the Effects of Prostaglandin Analogues on Cultured Trabecular Meshwork Cells by Coherent Anti-Stokes Raman Scattering

2013 ◽  
Vol 54 (9) ◽  
pp. 5972 ◽  
Author(s):  
Tim C. Lei ◽  
Omid Masihzadeh ◽  
Malik Y. Kahook ◽  
David A. Ammar
Keyword(s):  
Raman Scattering ◽  
Trabecular Meshwork ◽  
Prostaglandin Analogues ◽  
Trabecular Meshwork Cells ◽  
Anti Stokes

Effects of Oxidative Stress in Trabecular Meshwork Cells Are Reduced by Prostaglandin Analogues

2008 ◽  
Vol 49 (11) ◽  
pp. 4872 ◽  
Author(s):  
Alice L. Yu ◽  
Rudolf Fuchshofer ◽  
Anselm Kampik ◽  
Ulrich Welge-Lu¨ssen
Keyword(s):  
Oxidative Stress ◽  
Trabecular Meshwork ◽  
Prostaglandin Analogues ◽  
Trabecular Meshwork Cells

Effects of Prostaglandin Analogues on Human Ciliary Muscle and Trabecular Meshwork Cells

2003 ◽  
Vol 44 (5) ◽  
pp. 1945 ◽  
Author(s):  
Xiujun Zhao ◽  
Keri E. Pearson ◽  
Dietrich A. Stephan ◽  
Paul Russell
Keyword(s):  
Trabecular Meshwork ◽  
Ciliary Muscle ◽  
Prostaglandin Analogues ◽  
Trabecular Meshwork Cells

Mechanical stretch upregulates connexin43 in human trabecular meshwork cells

2019 ◽  
Author(s):  
Nikoleta Tellios ◽  
Mary Feng ◽  
Nancy Chen ◽  
Hong Liu ◽  
Vasiliki Tellios ◽  
...  
Keyword(s):  
Trabecular Meshwork ◽  
Mechanical Stretch ◽  
Trabecular Meshwork Cells

scholarly journals Selective Imaging of Microplastic and Organic Particles in Flow by Multimodal Coherent Anti-Stokes Raman Scattering and Two-Photon Excited Autofluorescence Analysis

2021 ◽  
Vol 93 (12) ◽  
pp. 5234-5240
Author(s):  
Tomoko Takahashi ◽  
Krzysztof Pawel Herdzik ◽  
Konstantinos Nikolaos Bourdakos ◽  
James Arthur Read ◽  
Sumeet Mahajan
Keyword(s):  
Raman Scattering ◽  
Two Photon ◽  
Organic Particles ◽  
Anti Stokes

Effect of dexamethasone on the expression of MMPs, adenosine A1 receptors and NFKB by human trabecular meshwork cells

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Normie Aida Mohd Nasir ◽  
Renu Agarwal ◽  
Anna Krasilnikova ◽  
Siti Hamimah Sheikh Abdul Kadir ◽  
Igor Iezhitsa
Keyword(s):  
Extracellular Matrix ◽  
Western Blot ◽  
Trabecular Meshwork ◽  
Culture Media ◽  
Smooth Muscle Actin ◽  
Adenosine A1 Receptors ◽  
Trabecular Meshwork Cells ◽  
Aqueous Humor Dynamics ◽  
Nfkb Activation ◽  
Adenosine A1

AbstractObjectivesSteroid-induced ocular hypertension and glaucoma are associated with extracellular matrix remodeling at the trabecular meshwork (TM) of the eye due to reduced secretion of matrix metalloproteinases (MMPs), a family of enzymes regulating extracellular matrix proteolysis. Several biological functions of steroids are known to involve regulation of adenosine A1 receptors (A1AR) and nuclear factor kappa B (NFKB). Since MMPs expression in TM has been shown to be regulated by A1AR as well as transcription factors, it is likely that dexamethasone-induced changes in aqueous humor dynamics involve reduced MMP and A1AR expression and reduced NFKB activation. Hence, the current study investigated the association of dexamethasone-induced reduction in MMP secretion with reduced NFKB activation and A1AR expression.MethodsHuman trabecular meshwork cells (HTMCs) were characterized by estimating myocilin and alpha smooth muscle actin expression and then were treated with dexamethasone 100 nM for 2, 5 and 7 days. The MMP secretion was estimated in culture media using Western blot. Immunocytochemistry (ICC) and ELISA were done to investigate the effect of dexamethasone on NFKB phosphorylation. A1AR expression in HTMCs was determined using Western blot and ELISA.ResultsDexamethasone caused a significant reduction in both MMP-2 and -9 expression compared to untreated group after five and seven days but not after two days of culture. Significantly reduced phosphorylated NFKB and A1AR protein levels were detected in dexamethasone treated compared to vehicle treated HTMCs after five days of culture.ConclusionsDexamethasone reduces MMP-2 and -9 secretion by HTMCs and this effect of dexamethasone is associated with reduced NFKB phosphorylation and A1AR expression.

scholarly journals Anti-Stokes Raman Scattering of Single Carbyne Chains

ACS Nano ◽  
2021 ◽  
Author(s):  
Cla Duri Tschannen ◽  
Martin Frimmer ◽  
Georgy Gordeev ◽  
Thiago L. Vasconcelos ◽  
Lei Shi ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Anti Stokes
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