Effects of Simvastatin on Retinal Structure and Function of a High-Fat Atherogenic Mouse Model of Thickened Bruch's Membrane

2014 ◽  
Vol 55 (1) ◽  
pp. 460 ◽  
Author(s):  
Veluchamy A. Barathi ◽  
Sia W. Yeo ◽  
Robyn H. Guymer ◽  
Tien Y. Wong ◽  
Chi D. Luu
Keyword(s):  
Mouse Model ◽  
Structure And Function ◽  
High Fat ◽  
Bruch's Membrane ◽  
Bruch’S Membrane ◽  
Retinal Structure ◽  
And Function

scholarly journals Effects of high-fat diet and Apoe deficiency on retinal structure and function in mice

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Xiupeng Cao ◽  
Yatu Guo ◽  
Yuchuan Wang ◽  
Hao Wang ◽  
Dong Liu ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Ganglion Cells ◽  
Structure And Function ◽  
Retinal Function ◽  
Retinal Vasculature ◽  
Mrna Levels ◽  
High Fat ◽  
Tnf Α ◽  
Retinal Structure ◽  
And Function

Abstract To investigate the effects of a high-fat diet (HFD) and apolipoprotein E (Apoe) deficiency on retinal structure and function in mice. Apoe KO mice and wild-type C57BL/6J mice were given a low-fat diet (LFD) or a HFD for 32 weeks. Blood glucose, serum lipids, body weight and visceral fat weight were evaluated. Retinal sterol quantification was carried out by isotope dilution gas chromatography-mass spectrometry. The cholesterol metabolism related genes SCAP-SREBP expressions were detected by qRT-PCR. Retinal function was recorded using an electroretinogram. The thickness of each layer of the retina was measured by optical coherence tomography. Fundus fluorescein angiography was performed to detect retinal vasculature changes. Immunohistochemical staining was used to determine the expression of NF-κB, TNF-α and VEGFR2 in the retina among HFD, HFD Apoe−/−, LFD Apoe−/− and WT mice retinas. HFD feeding caused the mice to gain weight and develop hypercholesterinemia, while Apoe−/− abnormalities also affected blood lipid metabolism. Both HFD and Apoe deficiency elevated retinal cholesterol, especially in the HFD Apoe−/− mice. No up-regulated expression of SCAP-SREBP was observed as a negative regulator. Impaired retinal functions, thinning retinas and abnormal retinal vasculature were observed in the peripheral retinas of the HFD and Apoe−/− mice compared with those in the normal chow group, particularly in the HFD Apoe−/− mice. Moreover, the expression of NF-κB in the retinas of the HFD and Apoe−/− mice was increased, together with upregulated TNF-α mRNA levels and TNF-α expression in the layer of retinal ganglion cells of the peripheral retina. At the same time, the expression level of VEGFR2 was elevated in the intervention groups, most notably in HFD Apoe−/− mice. HFD or Apoe gene deletion had certain adverse effects on retinal function and structure, which were far below the combined factors and induced harm to the retina. Furthermore, HFD caused retinal ischemia and hypoxia. Additionally, Apoe abnormality increased susceptibility to ischemia. These changes upregulated NF-κB expression in ganglion cells and activated downstream TNF-α. Simultaneously, they activated VEGFR2, accelerating angiogenesis and vascular permeability. All of the aforementioned outcomes initiated inflammatory responses to trigger ganglion cell apoptosis and aggravate retinal neovascularization.

scholarly journals Gene Therapy Preserves Retinal Structure and Function in a Mouse Model of NMNAT1-Associated Retinal Degeneration

2020 ◽  
Vol 18 ◽  
pp. 582-594
Author(s):  
Scott H. Greenwald ◽  
Emily E. Brown ◽  
Michael J. Scandura ◽  
Erin Hennessey ◽  
Raymond Farmer ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Mouse Model ◽  
Retinal Degeneration ◽  
Structure And Function ◽  
Retinal Structure ◽  
And Function

Metagenomics approach to the intestinal microbiome structure and function in high fat diet-induced obesity in mice fed with conjugated linoleic acid (CLA)

2020 ◽  
Vol 11 (11) ◽  
pp. 9729-9739
Author(s):  
Lina Liu ◽  
Yingying He ◽  
Kai Wang ◽  
Jinlai Miao ◽  
Zhou Zheng
Keyword(s):  
Linoleic Acid ◽  
Mouse Model ◽  
Conjugated Linoleic Acid ◽  
High Fat Diet ◽  
Structure And Function ◽  
Intestinal Microbiome ◽  
High Fat ◽  
Diet Induced Obesity ◽  
Obesity In Mice ◽  
And Function

In this study, a high fat diet induced obesity mouse model (DIO) was used to investigate the modulatory effect of high purity conjugated linoleic acid (CLA) on the intestinal microbiota.

XIAP gene therapy effects on retinal ganglion cell structure and function in a mouse model of glaucoma

Gene Therapy ◽  
2021 ◽  
Author(s):  
Shagana Visuvanathan ◽  
Adam N. Baker ◽  
Pamela S. Lagali ◽  
Stuart G. Coupland ◽  
Garfield Miller ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Mouse Model ◽  
Ganglion Cell ◽  
Retinal Ganglion Cell ◽  
Cell Structure ◽  
Structure And Function ◽  
Retinal Ganglion ◽  
And Function

Retinal Structure and Function in Perinatally HIV-Infected and cART-Treated Children: A Matched Case–Control Study

2015 ◽  
Vol 56 (6) ◽  
pp. 3945 ◽  
Author(s):  
Nazli Demirkaya ◽  
Sophie Cohen ◽  
Ferdinand W. N. M. Wit ◽  
Michael D. Abramoff ◽  
Reinier O. Schlingemann ◽  
...  
Keyword(s):  
Case Control Study ◽  
Case Control ◽  
Structure And Function ◽  
Matched Case ◽  
Retinal Structure ◽  
And Function ◽  
Matched Case Control Study ◽  
Control Study
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