Moderately Reduced ATP Levels Promote Oxidative Stress and Debilitate Autophagic and Phagocytic Capacities in Human RPE Cells

2012 ◽  
Vol 53 (9) ◽  
pp. 5354 ◽  
Author(s):  
Florian Schütt ◽  
Sebastian Aretz ◽  
Gerd U. Auffarth ◽  
Jürgen Kopitz
Keyword(s):  
Oxidative Stress ◽  
Rpe Cells ◽  
Atp Levels ◽  
Human Rpe Cells

UV-A induced oxidative stress is more prominent in naturally pigmented aged human RPE cells compared to non-pigmented human RPE cells independent of zinc treatment

2008 ◽  
Vol 90 (2) ◽  
pp. 113-120 ◽  
Author(s):  
Antje Biesemeier ◽  
Despina Kokkinou ◽  
Sylvie Julien ◽  
Peter Heiduschka ◽  
Mark Berneburg ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Zinc Treatment ◽  
Rpe Cells ◽  
Human Rpe Cells

scholarly journals Human Retinal Pigment Epithelial Cells Overexpressing the Neuroprotective Proteins PEDF and GM-CSF to Treat Degeneration of the Neural Retina

2021 ◽  
Vol 21 ◽  
Author(s):  
Thais Bascuas ◽  
Hajer Zedira ◽  
Martina Kropp ◽  
Nina Harmening ◽  
Mohamed Asrih ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Delivery ◽  
Retinal Pigment Epithelial ◽  
Retinal Pigment ◽  
Sleeping Beauty ◽  
Rpe Cells ◽  
Gm Csf ◽  
Sleeping Beauty Transposon ◽  
Stress Damage ◽  
Human Rpe Cells

Background: Non-viral transposon-mediated gene delivery can overcome viral vectors’ limitations. Transposon gene delivery offers the safe and life-long expression of genes such as pigment epithelium-derived factor (PEDF) and granulocyte-macrophage colony-stimulating factor (GM-CSF) to counteract retinal degeneration by reducing oxidative stress damage. Objective: Use Sleeping Beauty transposon to transfect human retinal pigment epithelial (RPE) cells with the neuroprotective factors PEDF and GM-CSF to investigate the effect of these factors on oxidative stress damage. Methods: Human RPE cells were transfected with PEDF and GM-CSF by electroporation, using the hyperactive Sleeping Beauty transposon gene delivery system (SB100X). Gene expression was determined by RT-qPCR and protein level by Western Blot as well as ELISA. The cellular stress level and the neuroprotective effect of the proteins were determined by measuring the concentrations of the antioxidant glutathione in human RPE cells and immunohistochemical examination of retinal integrity, inflammation, and apoptosis of rat retina-organotypic cultures (ROC) exposed to H2O2. Results: Human RPE cells were efficiently transfected, showing a significantly augmented gene expression and protein secretion. Human RPE cells overexpressing PEDF and/or GM-CSF or pre-treated with recombinant proteins presented significantly increased glutathione levels post-H2O2 incubation than non-transfected/untreated controls. rPEDF and/or rGM-CSF-treated ROC exhibited decreased inflammatory reactions and cell degeneration. Conclusion: GM-CSF and/or PEDF could be delivered successfully to RPE cells by combining the use of SB100X and electroporation. PEDF and/or GM-CSF reduced H2O2-mediated oxidative stress damage in RPE cells and ROC offering an encouraging technique to re-establish a cell-protective environment to halt age-related retinal degeneration.

Docosahexaenoic acid modulates oxidative stress-induced apoptosis via PI3K/Akt m-TOR/p70S6K pathways in human RPE cells

2015 ◽  
Vol 117 (6) ◽  
pp. 786-796 ◽  
Author(s):  
Narjes Babchia ◽  
Aline DeAraujo ◽  
Laurent Leclère ◽  
Bénédicte Buteau ◽  
Lucy Martine ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Docosahexaenoic Acid ◽  
Rpe Cells ◽  
Induced Apoptosis ◽  
Human Rpe Cells

scholarly journals The effects of quercetin in cultured human RPE cells under oxidative stress and in Ccl2/Cx3cr1 double deficient mice

2010 ◽  
Vol 91 (1) ◽  
pp. 15-25 ◽  
Author(s):  
Xiaoguang Cao ◽  
Melissa Liu ◽  
Jingsheng Tuo ◽  
Defen Shen ◽  
Chi-Chao Chan
Keyword(s):  
Oxidative Stress ◽  
Rpe Cells ◽  
Deficient Mice ◽  
Human Rpe Cells

scholarly journals Indocyanine green increases light-induced oxidative stress, senescence, and matrix metalloproteinases 1 and 3 in human RPE cells

2010 ◽  
Vol 90 (6) ◽  
pp. 571-579 ◽  
Author(s):  
Marcus Kernt ◽  
Christoph Hirneiss ◽  
Armin Wolf ◽  
Raffael Liegl ◽  
Johann Rueping ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Matrix Metalloproteinases ◽  
Indocyanine Green ◽  
Rpe Cells ◽  
Human Rpe Cells

Docosahexaenoic acid protects human RPE cells against oxidative stress via PI3K/Akt m-TOR/p70-p85S6K pathways

2012 ◽  
Vol 90 ◽  
pp. 0-0 ◽  
Author(s):  
N BABCHIA ◽  
A DE ARAUJO ◽  
L LECLERE ◽  
B BUTEAU ◽  
S GREGOIRE ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Docosahexaenoic Acid ◽  
Rpe Cells ◽  
Human Rpe Cells

scholarly journals Protective Effect of Melatonin against Oxidative Stress-Induced Apoptosis and Enhanced Autophagy in Human Retinal Pigment Epithelium Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Chih-Chao Chang ◽  
Tien-Yi Huang ◽  
Hsin-Yuan Chen ◽  
Tsui-Chin Huang ◽  
Li-Chun Lin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Oxidative Damage ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Critical Role ◽  
Age Related Macular Degeneration ◽  
Cell Degeneration ◽  
Rpe Cells ◽  
Age Related ◽  
Human Rpe Cells

Age-related macular degeneration (AMD) affects the retinal macula and results in loss of vision, and AMD is the primary cause of blindness and severe visual impairment among elderly people worldwide. AMD is characterized by the accumulation of drusen in the Bruch’s membrane and dysfunction of retinal pigment epithelial (RPE) cells and photoreceptors. The pathogenesis of AMD remains unclear, and no effective treatment exists. Accumulating evidence indicates that oxidative stress plays a critical role in RPE cell degeneration and AMD. Melatonin is an antioxidant that scavenges free radicals, and it has anti-inflammatory, antitumor, and antiangiogenic effects. This study investigated the antioxidative, antiapoptotic, and autophagic effects of melatonin on oxidative damage to RPE cells. We used hydrogen peroxide (H2O2) to stimulate reactive oxygen species production to cause cell apoptosis in ARPE-19 cell lines. Our findings revealed that treatment with melatonin significantly inhibited H2O2-induced RPE cell damage, decreased the apoptotic rate, increased the mitochondrial membrane potential, and increased the autophagy effect. Furthermore, melatonin reduced the Bax/Bcl-2 ratio and the expression levels of the apoptosis-associated proteins cytochrome c and caspase 7. Additionally, melatonin upregulated the expression of the autophagy-related proteins LC3-II and Beclin-1 and downregulated the expression of p62. Thus, melatonin’s effects on autophagy and apoptosis can protect against H2O2-induced oxidative damage in human RPE cells. Melatonin may have multiple protective effects on human RPE cells against H2O2-induced oxidative damage.

scholarly journals The Effect of Quercetin in Cultured Human RPE Cells under Oxidative Stress and in Ccl2/Cx3cr1 Double Deficient Mice

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Xiaoguang Cao ◽  
Jingsheng Tuo ◽  
Defen Shen ◽  
Melissa Liu ◽  
Chi‐Chao Chan
Keyword(s):  
Oxidative Stress ◽  
Rpe Cells ◽  
Deficient Mice ◽  
Human Rpe Cells

Oxidative Stress-Mediated Induction of MMP-1 and MMP-3 in Human RPE Cells

2009 ◽  
Vol 50 (11) ◽  
pp. 5495 ◽  
Author(s):  
Claudia S. Alge-Priglinger ◽  
Thomas Kreutzer ◽  
Katja Obholzer ◽  
Armin Wolf ◽  
Martin Mempel ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Rpe Cells ◽  
Human Rpe Cells
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