scholarly journals A Novel Fluorescent Lipid Probe for Dry Eye: Retrieval by Tear Lipocalin in Humans

2013 ◽  
Vol 54 (2) ◽  
pp. 1398 ◽  
Author(s):  
Po-Ting Yeh ◽  
Richard Casey ◽  
Ben J. Glasgow
Keyword(s):  
Dry Eye ◽  
Tear Lipocalin ◽  
Lipid Probe ◽  
Fluorescent Lipid

Tear Lipocalin and Lysozyme in Sj??gren and Non-Sjogren Dry Eye

2008 ◽  
Vol 85 (8) ◽  
pp. 661-667 ◽  
Author(s):  
Barbary Caffery ◽  
Elizabeth Joyce ◽  
Adrienne Boone ◽  
Allan Slomovic ◽  
Trefford Simpson ◽  
...  
Keyword(s):  
Dry Eye ◽  
Tear Lipocalin

scholarly journals Measurement of LDL particle size in whole plasma and serum by high performance gel-filtration chromatography using a fluorescent lipid probe

1998 ◽  
Vol 44 (10) ◽  
pp. 2148-2151 ◽  
Author(s):  
Peter G Scheffer ◽  
Stephan J L Bakker ◽  
Robert J Heine ◽  
Tom Teerlink
Keyword(s):  
High Performance ◽  
Gel Filtration ◽  
Uv Detection ◽  
Size Measurement ◽  
Gel Filtration Chromatography ◽  
Modified Method ◽  
Ldl Size ◽  
Parinaric Acid ◽  
Lipid Probe ◽  
Fluorescent Lipid

Abstract We have recently described a technique for measuring LDL size by high performance gel-filtration chromatography (HPGC) with UV detection (Scheffer et al., Clin Chem 1997;43:1904–12). A drawback of this method is the necessity of LDL isolation before chromatography. We now describe a modification of this method based on selective detection of lipoproteins by postcolumn labeling with parinaric acid, a fluorescent lipid probe. Measuring the size of isolated LDL by HPGC in 56 subjects, we obtained diameters of 25.72 ± 0.60 nm with UV detection and of 25.74 ± 0.58 nm with fluorescence detection. The modified method is suitable for LDL size measurement in whole plasma or serum. LDL sizes measured in whole plasma correlated strongly with the respective values in isolated LDL (r = 0.976) but were on average 0.18 nm larger (P < 0.001). CVs for within- and between-series imprecision were <0.25%. The present method requires only 5 μL plasma or serum without sample preparation and is suitable for the unattended analysis of large series of samples.

Dry Eye Responds Best to Combination Therapy

2008 ◽  
Vol 39 (2) ◽  
pp. 69
Author(s):  
CAROLINE HELWICK
Keyword(s):  
Combination Therapy ◽  
Dry Eye
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