Fully Automated Montaging of Laser Scanning In Vivo Confocal Microscopy Images of the Human Corneal Subbasal Nerve Plexus

2012 ◽  
Vol 53 (4) ◽  
pp. 2235 ◽  
Author(s):  
Jason T. Turuwhenua ◽  
Dipika V. Patel ◽  
Charles N. J. McGhee
Keyword(s):  
Confocal Microscopy ◽  
Laser Scanning ◽  
Nerve Plexus ◽  
In Vivo Confocal Microscopy ◽  
Microscopy Images ◽  
Subbasal Nerve Plexus

Image Reconstruction of the Subbasal Nerve Plexus with In Vivo Confocal Microscopy

2011 ◽  
Vol 52 (9) ◽  
pp. 5022 ◽  
Author(s):  
Stephan Allgeier ◽  
Andrey Zhivov ◽  
Franz Eberle ◽  
Bernd Koehler ◽  
Susanne Maier ◽  
...  
Keyword(s):  
Image Reconstruction ◽  
Confocal Microscopy ◽  
Nerve Plexus ◽  
In Vivo Confocal Microscopy ◽  
Subbasal Nerve Plexus

scholarly journals An in vivo confocal microscopy study of corneal changes in patients with systemic sclerosis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Eszter Szalai ◽  
Gabriella Szucs ◽  
Szilvia Szamosi ◽  
Zsuzsa Aszalos ◽  
Ildiko Afra ◽  
...  
Keyword(s):  
Systemic Sclerosis ◽  
Confocal Microscopy ◽  
Ocular Surface ◽  
Healthy Subjects ◽  
Ocular Surface Disease ◽  
Nerve Plexus ◽  
In Vivo Confocal Microscopy ◽  
Software Analysis ◽  
Subbasal Nerve Plexus

AbstractTo investigate corneal microstructure of systemic sclerosis (SSc) patients using in vivo confocal microscopy (IVCM). 33 patients with SSc and 30 age-matched healthy subjects were recruited. All participants underwent comprehensive ophthalmic examination including IVCM (Heidelberg Retina Tomograph III, Heidelberg Engineering GmbH, Heidelberg, Germany) and ocular surface evaluation. Subbasal nerve plexus morphology was investigated using automated software analysis (ACCMetrics V3; University of Manchester, Manchester, UK). Keratocyte cell densities in the anterior stroma were significantly lower in patients with SSc compared to controls (P < 0.0001). In 7 SSc patients no keratocyte nuclei were identified in the anterior stroma and in most patients scattered hyperreflective punctate material were observed in the anterior stroma. Significantly lower subbasal nerve fiber parameters were found in patients with SSc compared to healthy subjects (P < 0.05). There were no significant correlations between the duration of SSc and any of the corneal cell density values. Tear break-up time values (4.82 ± 3.15 s) and Ocular Surface Disease Index scores (33.27 ± 30.11) were abnormal, Schirmer values (6.78 ± 5.82 mm) were borderline in SSc patients. In SSc, corneal morphological changes and accumulation of punctate material in the stroma was detected with confocal microscopy. Severe ocular surface disease was observed in SSc patients with significant impairment in subbasal nerve plexus morphology resembling peripheral neuropathy.

scholarly journals Laser scanning in vivo confocal microscopy (IVCM) for evaluating human corneal sub-basal nerve plexus parameters: protocol for a systematic review

BMJ Open ◽  
2017 ◽  
Vol 7 (11) ◽  
pp. e018646 ◽  
Author(s):  
Manikkuwadura Eranda Harshan De Silva ◽  
Alexis Ceecee Zhang ◽  
Amalia Karahalios ◽  
Holly Rose Chinnery ◽  
Laura Elizabeth Downie
Keyword(s):  
Systematic Review ◽  
Confocal Microscopy ◽  
Laser Scanning ◽  
Meta Analysis ◽  
Nerve Plexus ◽  
Cochrane Library ◽  
Published Data ◽  
In Vivo Confocal Microscopy ◽  
Corneal Nerve

IntroductionLaser scanning in vivo confocal microscopy (IVCM) enables non-invasive, high-resolution imaging of the cornea. In recent years, there has been a vast increase in researchers using laser scanning IVCM to image and quantify corneal nerve parameters. However, a range of methodological approaches have been adopted. The primary aim of this systematic review is to critically appraise the reported method(s) of primary research studies that have used laser scanning IVCM to quantify corneal sub-basal nerve plexus (SBNP) parameters in humans, and to examine corneal nerve parameters in healthy individuals.Methods and analysisA systematic review of primary studies that have used laser scanning IVCM to quantify SBNP parameters in humans will be conducted. Comprehensive electronic searches will be performed in Ovid MedLine, Embase and the Cochrane Library. Two reviewers will independently assess titles and abstracts, and exclude studies not meeting the inclusion criteria. For studies judged eligible or potentially eligible, full texts will be independently assessed by two reviewers to determine eligibility. A third reviewer will resolve any discrepancies in judgement. Risk of bias will be assessed using a custom tool, covering five methodological domains: participant selection, method of image capture, method of image analysis, data reporting and other sources of bias. A systematic narrative synthesis of findings will be provided. A multilevel random-effects meta-analysis will be performed for corneal nerve parameters derived from healthy participants. This review will be reported according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses statement.Ethics and disseminationAs this review considers published data, ethical approval is not required. We foresee that this synthesis will serve as a reference for future studies, and can be used to inform best practice standards for using IVCM in clinical research. A manuscript reporting the results of the review will be published and may also be presented at scientific conferences.

scholarly journals Effects of Fluorescein Staining on Laser In Vivo Confocal Microscopy Images of the Cornea

2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
Christine W. Sindt ◽  
D. Brice Critser ◽  
Trudy K. Grout ◽  
Jami R. Kern
Keyword(s):  
Confocal Microscopy ◽  
Contact Lens ◽  
Immune Cells ◽  
In Vivo Confocal Microscopy ◽  
Eye Care ◽  
Laser Confocal Microscopy ◽  
Fluorescein Staining ◽  
Microscopy Images ◽  
Subbasal Nerve Plexus

This study was designed to identify whether topical fluorescein, a common ophthalmic tool, affects laser in vivo confocal microscopy of the cornea, a tool with growing applications. Twenty-five eye care specialists were asked to identify presence or absence of fluorescein in 99 confocal micrographs of healthy corneas. Responses were statistically similar to guessing for the epithelium (48% ± 14% of respondents correct per image) and the subbasal nerve plexus (49% ± 11% correct), but results were less clear for the stroma. Dendritic immune cells were quantified in bilateral images from subjects who had been unilaterally stained with fluorescein. Density of dendritic immune cells was statistically similar between the unstained and contralateral stained eyes of 24 contact lens wearers (P=.72) and of 10 nonwearers (P=.53). Overall, the results indicated that fluorescein staining did not interfere with laser confocal microscopy of corneal epithelium, subbasal nerves, or dendritic immune cells.

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