Multiplex Cytokine Analysis of Aqueous Humor in Eyes with Primary Open-Angle Glaucoma, Exfoliation Glaucoma, and Cataract

2012 ◽  
Vol 53 (1) ◽  
pp. 241 ◽  
Author(s):  
Yasuyuki Takai ◽  
Masaki Tanito ◽  
Akihiro Ohira
Keyword(s):  
Aqueous Humor ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Open Angle ◽  
Exfoliation Glaucoma ◽  
Cytokine Analysis ◽  
Multiplex Cytokine

scholarly journals Evaluation of Redox Profiles of the Serum and Aqueous Humor in Patients with Primary Open-Angle Glaucoma and Exfoliation Glaucoma

Antioxidants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1305
Author(s):  
Yuji Takayanagi ◽  
Yasuyuki Takai ◽  
Sachiko Kaidzu ◽  
Masaki Tanito
Keyword(s):  
Oxidative Stress ◽  
Aqueous Humor ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Lipid Peroxides ◽  
Redox Status ◽  
Reactive Oxygen Metabolites ◽  
Open Angle ◽  
Blood Samples ◽  
Exfoliation Glaucoma

Oxidative stress is thought to play a significant role in the development of glaucoma. However, the association between systemic and local oxidative stresses in different types of glaucoma has not been assessed fully. The current study compared the redox status in the aqueous humor (AH) and blood samples among eyes with primary open-angle glaucoma (POAG), exfoliation glaucoma (EXG), and non-glaucomatous controls to evaluate the relationship among systemic redox status, intraocular oxidative stress, and clinical backgrounds. AH and blood samples were obtained from 45 eyes of 45 Japanese subjects (15 POAG, 15 EXG, and 15 control eyes). The serum levels of lipid peroxides, ferric-reducing activity, and thiol antioxidant activity were measured by diacron reactive oxygen metabolites (dROM), biologic antioxidant potential (BAP), and sulfhydryl (SH) tests, respectively, using a free radical analyzer. The activities of cytosolic and mitochondrial forms of the superoxide dismutase (SOD) isoforms, i.e., SOD1 and SOD2, respectively, in AH and serum were measured using a multiplex bead immunoassay. In AH, SOD1 in subjects with EXG and SOD2 in those with POAG and EXG were significantly higher than in control eyes. In serum, compared to control subjects, BAP in subjects with POAG and EXG was significantly lower; SOD1 in those with EXG and SOD2 in those with POAG and EXG were significantly higher. dROM and SH did not differ significantly among the groups. The BAP values were correlated negatively with the SOD1 concentrations in AH and serum, SOD2 in the AH, intraocular pressure, and number of antiglaucoma medications. In conclusion, lower systemic antioxidant capacity accompanies up-regulation of higher local antioxidant enzymes, suggesting increased oxidative stress in eyes with OAG, especially in EXG. Determination of the systemic BAP values may help predict the redox status in AH.

scholarly journals Differentially expressed microRNAs in the aqueous humor of patients with exfoliation glaucoma or primary open-angle glaucoma

2018 ◽  
Vol 27 (7) ◽  
pp. 1263-1275 ◽  
Author(s):  
Michelle D Drewry ◽  
Pratap Challa ◽  
John G Kuchtey ◽  
Iris Navarro ◽  
Inas Helwa ◽  
...  
Keyword(s):  
Aqueous Humor ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Differentially Expressed ◽  
Open Angle ◽  
Exfoliation Glaucoma

Detection of specific proteins in the aqueous humor in primary open-angle glaucoma

1990 ◽  
Vol 4 (1) ◽  
pp. 1 ◽  
Author(s):  
In Seop Lee ◽  
Young Suk Yu ◽  
Dong Myung Kim ◽  
Dong Ho Youn ◽  
Jin Q Kim
Keyword(s):  
Aqueous Humor ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Open Angle ◽  
Specific Proteins

scholarly journals Profile of vascular and inflammatory aqueous humor cytokines in primary open angle glaucoma patients

2019 ◽  
Author(s):  
Ruyi Zhai ◽  
Huan Xu ◽  
Xinghuai Sun ◽  
Xiangmei Kong
Keyword(s):  
Aqueous Humor ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Antibody Array ◽  
Open Angle ◽  
Aqueous Samples ◽  
Age Related ◽  
Tnf Α ◽  
Cytokine Antibody Array ◽  
Angiopoietin 2

Abstract Purpose: This study was aimed to analyze the profile of vascular and inflammatory cytokines in aqueous humor of primary open angle glaucoma (POAG) patients. Methods: Aqueous humor samples were acquired from 6 POAG patients and 7 age-related cataract patients that constituted the control group. A human cytokine antibody array was used to detect 37 proteins related to inflammation and vascular regulation. Characteristics including age, gender, and intraocular pressure (IOP) of POAG and age-related cataract patients were compared. Correlation analyses between aqueous humor cytokines and characteristics were performed. Results: The cytokine antibody array results showed that the signal intensities of soluble vascular endothelial growth factor receptor 1 (sVEGFR-1), von Willebrand factor (vWF), tumor necrosis factor (TNF)-α, and angiopoietin-2 in POAG patients were significantly higher in aqueous samples compared to controls (p < 0.05, Student’s test or Mann-Whitney U-test). Among cytokines of all aqueous samples, preoperative IOP was positively associated with expression quantity of sVEGFR-1 (r = 0.647, p = 0.02) and VEGF-A (r = 0.602, p = 0.04) and negatively associated with plasminogen activator inhibitor 1 expression (r = -0.593, p = 0.04). Values for sVEGFR-1 were positively correlated with vWF (r = 0.646, p = 0.02) and TNF-α (r = 0.824, p < 0.001) with statistical significance. Conclusion: Pathogenesis of primary open angle glaucoma may be associated with both vascular dysfunction and inflammatory responses. The vascular factors may include endothelial dysfunction and damaged vascular permeability as indicated by abnormal expression of vWF, sVEGFR-1 and angiopoietin-2. Keywords: POAG, aqueous humor, cytokines, sVEGFR-1, vWF, angiopoietin-2

scholarly journals Comparison of cytokine/chemokine levels in aqueous humor of primary open-angle glaucoma patients with positive or negative outcome following trabeculectomy

2019 ◽  
Vol 39 (5) ◽  
Author(s):  
Beata Gajda-Deryło ◽  
Thomas Stahnke ◽  
Stephan Struckmann ◽  
Gregor Warsow ◽  
Kerstin Birke ◽  
...  
Keyword(s):  
Growth Factor ◽  
Aqueous Humor ◽  
Transforming Growth Factor ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Transforming Growth Factor Β ◽  
Positive Outcome ◽  
Open Angle ◽  
Antifibrotic Therapy ◽  
Cytokines And Chemokines

Abstract We aimed to identify differences in cytokine/chemokine levels in the aqueous humor (AH) of primary open-angle glaucoma (POAG) patients who suffered from scarring, compared with POAG patients with no scarring after trabeculectomy surgery. Identification of differently expressed cytokines and chemokines may help to understand scarring and fibrotic processes following trabeculectomy, and to make predictions for the outcome of fistulating surgery in the future. Furthermore, the identification of cell signaling pathways involved in fibrosis offers the opportunity for a more specific antifibrotic therapy with reduced side effects, and an improvement in long-term surgical outcome. Eight samples of AH were collected during trabeculectomy surgery and commercially available cytokine/chemokine arrays were used. Specific, differently expressed proteins (cytokines/chemokines) in AH samples from patients with positive and negative surgery outcomes were detected. These proteins were classified based on their known profibrotic, inflammatory, adhesive, and apoptotic properties. Transforming growth factor β (TGF-β) and vascular endothelial growth factor (VEGF) were among the most important profibrotic cytokines that we detected. Differences in the fold change of protein expression were highly significant between patients after successful and failed trabeculectomy surgery, and these were processed and visualized using ExprEssence software. This pilot study revealed differences in concentrations of cytokines/chemokines in AH between the two examined groups of patients. Our findings suggest that a positive outcome from trabeculectomy is strongly related to an inhibition of the fibrosis process.

Aqueous humor metabolite profile of pseudoexfoliation glaucoma is distinctive

2020 ◽  
Vol 16 (5) ◽  
pp. 425-435 ◽  
Author(s):  
Ciara Myer ◽  
Leila Abdelrahman ◽  
Santanu Banerjee ◽  
Ram B. Khattri ◽  
Matthew E. Merritt ◽  
...  
Keyword(s):  
Machine Learning ◽  
Aqueous Humor ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Metabolite Profile ◽  
Open Angle ◽  
Pseudoexfoliation Glaucoma

We identified 298 metabolites in pseudoexfoliation (PEX) glaucoma, primary open angle glaucoma (POAG) and non-glaucomatous controls. Machine learning can classify aqueous humor into the three distinct categories and presents the opportunity for future predictions.

Hypophosphorylation of Aqueous Humor sCD44 and Primary Open-Angle Glaucoma

2005 ◽  
Vol 46 (8) ◽  
pp. 2829 ◽  
Author(s):  
Paul A. Knepper ◽  
Adam M. Miller ◽  
John Choi ◽  
Robert D. Wertz ◽  
Michael J. Nolan ◽  
...  
Keyword(s):  
Aqueous Humor ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Open Angle

scholarly journals Differential metabolic markers associated with primary open-angle glaucoma and cataract in human aqueous humor

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Chen-Wei Pan ◽  
Chaofu Ke ◽  
Qin Chen ◽  
Yi-Jin Tao ◽  
Xu Zha ◽  
...  
Keyword(s):  
Aqueous Humor ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Open Angle ◽  
Metabolic Markers
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