Increased Integration of Transplanted CD73-Positive Photoreceptor Precursors into Adult Mouse Retina

2011 ◽  
Vol 52 (9) ◽  
pp. 6462 ◽  
Author(s):  
Dominic Eberle ◽  
Sandra Schubert ◽  
Kai Postel ◽  
Denis Corbeil ◽  
Marius Ader
Keyword(s):  
Adult Mouse ◽  
Mouse Retina

scholarly journals STAT pathway activation limits the Ascl1-mediated chromatin remodeling required for neural regeneration from Müller glia in adult mouse retina

2019 ◽  
Author(s):  
Nikolas L. Jorstad ◽  
Matthew S. Wilken ◽  
Levi Todd ◽  
Paul Nakamura ◽  
Nick Radulovich ◽  
...  
Keyword(s):  
Adult Mouse ◽  
Neural Regeneration ◽  
Mouse Retina ◽  
Binding Motif ◽  
Muller Glia ◽  
Müller Glia ◽  
Rna Seq ◽  
Transgenic Expression ◽  
Pathway Activation ◽  
Stat Pathway

AbstractMüller glia can serve as a source for retinal regeneration in some non-mammalian vertebrates. Recently we found that this process can be induced in mouse Müller glia after injury, by combining transgenic expression of the proneural transcription factor Ascl1 and the HDAC inhibitor TSA. However, new neurons are only generated from a subset of Müller glia in this model, and identifying factors that limit Ascl1-mediated MG reprogramming could potentially make this process more efficient, and potentially useful clinically. One factor that limits neurogenesis in some non-mammalian vertebrates is the STAT pathway activation that occurs in Müller glia in response to injury. In this report, we tested whether injury induced STAT activation hampers the ability of Ascl1 to reprogram Müller glia into retinal neurons. Using a STAT inhibitor, in combination with our previously described reprogramming paradigm, we found a large increase in the ability of Müller glia to generate neurons, similar to those we described previously. Single-cell RNA-seq showed that the progenitor-like cells derived from Ascl1-expressing Müller glia have a higher level of STAT signaling than those that become neurons. Using Ascl1 ChIP-seq and DNase-seq, we found that developmentally inappropriate Ascl1 binding sites (that were unique to the overexpression context) had enrichment for the STAT binding motif. This study provides evidence that STAT pathway activation reduces the efficiency of Ascl1-mediated reprogramming in Müller glia, potentially by directing Ascl1 to inappropriate targets.

scholarly journals Expression of the putative microRNA processing protein, Ars2, in the developing and adult mouse retina

2009 ◽  
Vol 331 (2) ◽  
pp. 511 ◽  
Author(s):  
Robert L. Chow ◽  
Phil E. Nickerson ◽  
Perry L. Howard
Keyword(s):  
Adult Mouse ◽  
Mouse Retina ◽  
Microrna Processing

scholarly journals Otx2 Gene Deletion in Adult Mouse Retina Induces Rapid RPE Dystrophy and Slow Photoreceptor Degeneration

PLoS ONE ◽  
2010 ◽  
Vol 5 (7) ◽  
pp. e11673 ◽  
Author(s):  
Francis Béby ◽  
Michael Housset ◽  
Nicolas Fossat ◽  
Coralie Le Greneur ◽  
Frédéric Flamant ◽  
...  
Keyword(s):  
Gene Deletion ◽  
Adult Mouse ◽  
Mouse Retina ◽  
Photoreceptor Degeneration

Coexistence of neurofilaments and vimentin in a neurone of adult mouse retina

Nature ◽  
1983 ◽  
Vol 303 (5913) ◽  
pp. 169-172 ◽  
Author(s):  
Ursula C. Dräger
Keyword(s):  
Adult Mouse ◽  
Mouse Retina

The guidance of optic axons in the developing and adult mouse retina

1979 ◽  
Vol 193 (4) ◽  
pp. 763-773 ◽  
Author(s):  
Stephen Goldberg ◽  
Beryn Frank
Keyword(s):  
Adult Mouse ◽  
Mouse Retina ◽  
Optic Axons

Microglial cells in organotypic cultures of developing and adult mouse retina and their relationship with cell death

2014 ◽  
Vol 121 ◽  
pp. 42-57 ◽  
Author(s):  
Rosa M. Ferrer-Martín ◽  
David Martín-Oliva ◽  
Ana Sierra ◽  
Maria-Carmen Carrasco ◽  
María Martín-Estebané ◽  
...  
Keyword(s):  
Cell Death ◽  
Adult Mouse ◽  
Microglial Cells ◽  
Mouse Retina ◽  
Organotypic Cultures
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