Different Populations of Circulating Endothelial Cells in Patients with Age-Related Macular Degeneration: A Novel Insight into Pathogenesis

2011 ◽  
Vol 52 (1) ◽  
pp. 93 ◽  
Author(s):  
Anna Machalinska ◽  
Krzysztof Safranow ◽  
Violetta Dziedziejko ◽  
Katarzyna Mozolewska-Piotrowska ◽  
Edyta Paczkowska ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Macular Degeneration ◽  
Age Related Macular Degeneration ◽  
Circulating Endothelial Cells ◽  
Age Related ◽  
Different Populations ◽  
Insight Into

Circulating endothelial and progenitor cells in age-related macular degeneration

2019 ◽  
Vol 30 (5) ◽  
pp. 956-965
Author(s):  
Dario Pasquale Mucciolo ◽  
Rossella Marcucci ◽  
Andrea Sodi ◽  
Francesca Cesari ◽  
Vittoria Murro ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Macular Degeneration ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cell ◽  
Progenitor Cell ◽  
Age Related Macular Degeneration ◽  
Circulating Endothelial Cells ◽  
Control Group ◽  
Endothelial Progenitor ◽  
Age Related

Purpose: To evaluate circulating endothelial and circulating progenitor cells as biomarkers in age-related macular degeneration patients (both exudative and atrophic forms) in order to establish the possible clinical implication of their assessment. Methods: We have enrolled 44 age-related macular degeneration patients: 22 patients with a recently diagnosed exudative (neovascular) form (Group A) and 22 patients with an atrophic (dry) form (Group B). The control group consisted of 22 age and sex-matched healthy subjects (Group C). The number of circulating endothelial progenitor cells (CD34+/KDR+, CD133+/KDR+, and CD34+/KDR+/CD133+), circulating progenitor cells (CD34+, CD133+, and CD34+/CD133+), and circulating endothelial cells were determined in the peripheral venous blood samples by flow cytometry. Neovascular age-related macular degeneration patients were evaluated at baseline and 4 weeks after a loading phase of three consequent intravitreal injections of ranibizumab. Results: Comparing age-related macular degeneration patients with the control group, endothelial progenitor cell and circulating progenitor cell levels were not significantly different, while age-related macular degeneration patients showed significantly higher levels of circulating endothelial cells ( p = 0.001). Anti–vascular endothelial growth factor treatment with intravitreal ranibizumab was associated with a significant reduction of endothelial progenitor cell levels, with no significant influence on circulating progenitor cells and circulating endothelial cells. Conclusion: We reported higher levels of circulating endothelial cells in age-related macular degeneration patients in comparison with the control group, thereby supporting the hypothesis of an involvement of endothelial dysregulation in the age-related macular degeneration and a reduction of the endothelial progenitor cell level in neovascular age-related macular degeneration patients after three intravitreal injections of ranibizumab.

scholarly journals Generating iPSC-Derived Choroidal Endothelial Cells to Study Age-Related Macular Degeneration

2015 ◽  
Vol 56 (13) ◽  
pp. 8258 ◽  
Author(s):  
Allison E. Songstad ◽  
Luke A. Wiley ◽  
Khahn Duong ◽  
Emily Kaalberg ◽  
Miles J. Flamme-Wiese ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Macular Degeneration ◽  
Age Related Macular Degeneration ◽  
Age Related

scholarly journals Single-cell transcriptomics of the human retinal pigment epithelium and choroid in health and macular degeneration

2019 ◽  
Vol 116 (48) ◽  
pp. 24100-24107 ◽  
Author(s):  
Andrew P. Voigt ◽  
Kelly Mulfaul ◽  
Nathaniel K. Mullin ◽  
Miles J. Flamme-Wiese ◽  
Joseph C. Giacalone ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Retinal Pigment Epithelium ◽  
Macular Degeneration ◽  
Single Cell ◽  
Rna Sequencing ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Age Related Macular Degeneration ◽  
Age Related ◽  
Single Cell Rna Sequencing

The human retinal pigment epithelium (RPE) and choroid are complex tissues that provide crucial support to the retina. Disease affecting either of these supportive tissues can lead to irreversible blindness in the setting of age-related macular degeneration. In this study, single-cell RNA sequencing was performed on macular and peripheral regions of RPE-choroid from 7 human donor eyes in 2 independent experiments. In the first experiment, total RPE/choroid preparations were evaluated and expression profiles specific to RPE and major choroidal cell populations were identified. As choroidal endothelial cells represent a minority of the total RPE/choroidal cell population but are strongly implicated in age-related macular degeneration (AMD) pathogenesis, a second single-cell RNA-sequencing experiment was performed using endothelial cells enriched by magnetic separation. In this second study, we identified gene expression signatures along the choroidal vascular tree, classifying the transcriptome of human choriocapillaris, arterial, and venous endothelial cells. We found that the choriocapillaris highly and specifically expresses the regulator of cell cycle gene (RGCC), a gene that responds to complement activation and induces apoptosis in endothelial cells. In addition, RGCC was the most up-regulated choriocapillaris gene in a donor diagnosed with AMD. These results provide a characterization of the human RPE and choriocapillaris transcriptome, offering potential insight into the mechanisms of choriocapillaris response to complement injury and choroidal vascular disease in age-related macular degeneration.

Hypocrellin B Encapsulated in Triphenyl Phosphonium-Modified Cationic Liposomes for Photodynamic Treatment of Exudative Age-Related Macular Degeneration

2019 ◽  
Vol 15 (12) ◽  
pp. 2305-2320
Author(s):  
Hongxia Chen ◽  
Hong Deng ◽  
Xianbiao Zou ◽  
Jingquan Zhao
Keyword(s):  
Endothelial Cells ◽  
Macular Degeneration ◽  
Vascular Endothelial Cells ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Age Related Macular Degeneration ◽  
Vascular Endothelial ◽  
Age Related ◽  
Triphenyl Phosphonium ◽  
Hypocrellin B

Verteporfin photodynamic therapy (PDT) has been approved for the treatment of exudative age-related macular degeneration (AMD) for over a decade. However, its extensive application has been impeded by inevitably collateral tissue damage and immediate induction of angiogenesis, in addition to the need of multiple treatments. In order to develop prospective photosensitizers for clinical use, a triphenyl phosphonium-modified cationic liposomal hypocrellin B (TPP cationic LHB) for angiogenic targeting and endothelial internalization was constructed. With optimal PDT parameters, TPP cationic LHB can lead to death of choroid-retinal vascular endothelial cells while cause negligible damage to collateral retinal pigment epithelium cells. This is likely due to the mitochondria targeting and effective intracellular singlet oxygen generation of TPP cationic LHB in vascular endothelial cells. Additionally, in vivo chick chorioallantoic membrane assay indicated the elevated neovessel-targeting ability and photo-induced anti-angiogenic activity of TPP cationic LHB. Furthermore, TPP cationic LHB PDT is able to maintain neovessel occlusion for an extended period of time compared with verteporfin PDT, without inducing significant increased expression of some angiogenic factors, such as vascular endothelial growth factor and integrin αvβ3. This study describes a facile strategy that may be useful for developing new-generation photosensitizers to circumvent the limitations of PDT treatment of exudative AMD.

scholarly journals Autophagy: A new insight into pathogenesis and treatment possibilities in age-related macular degeneration

2020 ◽  
Vol 74 ◽  
pp. 213-223
Author(s):  
Agnieszka Kubicka-Trząska ◽  
Izabella Karska-Basta ◽  
Katarzyna Żuber-Łaskawiec
Keyword(s):  
Macular Degeneration ◽  
Vision Loss ◽  
Developed Countries ◽  
Age Related Macular Degeneration ◽  
Therapeutic Modalities ◽  
Central Vision Loss ◽  
Age Related ◽  
Genetic And Environmental Factors ◽  
The Impact ◽  
Insight Into

Age-related macular degeneration (AMD) is a significant problem in healthcare, because it is a leading cause of central vision loss in individuals over 50 years old in well-developed countries. Pathogenesis of AMD is multifactorial and still not completely understood. Proven risk factors include the following: natural senescence of retina, oxidative stress, complement activation, chronic subretinal inflammatory reaction, genetic and environmental factors. Data on links between autophagy and AMD development are being raised. Autophagy is a cellular process involving the degradation of long-lived proteins and damaged fragments and components of cells; it is responsible for the maintenance of dynamic intracellular homeostasis and it enables cell survival under stress conditions. Disturbances of autophagy mechanisms, i.e. its activation or inhibition, may lead to the development of many various pathologies. Thus, autophagy plays a dual role, as a mechanism responsible for protecting or killing cells. The paper describes autophagy mechanisms and their role in the natural process of retinal cells senescence and presents the autophagy impairment as a crucial cause of AMD development. We also describe the impact of intravitreal anti-VEGF therapy on retinal autophagy mechanisms and potential new therapeutic modalities for AMD based on autophagy modulation.

scholarly journals Functional microRNA targetome undergoes degeneration-induced shift in the retina

2020 ◽  
Author(s):  
Joshua A. Chu-Tan ◽  
Zhi-Ping Feng ◽  
Yvette Wooff ◽  
Adrian V. Cioanca ◽  
Ulrike Schumann ◽  
...  
Keyword(s):  
Mouse Model ◽  
Neurodegenerative Diseases ◽  
Macular Degeneration ◽  
Retinal Degeneration ◽  
Significant Role ◽  
Age Related Macular Degeneration ◽  
Retinal Damage ◽  
Regulatory Activity ◽  
Age Related ◽  
Insight Into

SummaryMicroRNA (miRNA) play a significant role in the pathogenesis of complex neurodegenerative diseases, including age-related macular degeneration, acting as post-transcriptional gene suppressors through their association with argonaute (AGO) protein family members. However, to understand their role in disease, investigation into the regulatory nature of miRNA with their targets is required. To identify the active-miRnome-targetome interactions in the degenerating retina, AGO2 HITS-CLIP was performed using a mouse model of retinal degeneration. Analysis revealed a similar miRnome between healthy and damaged retinas, however, a shift in the active targetome was observed. This shift was also demonstrated by a change in the seed binding regions of miR-124-3p, the most abundant retinal miRNA loaded in AGO2. Following damage, AGO2 was localised to the inner retinal layers indicating a locational miRNA response to retinal damage. This study provides important insight into the alteration of miRNA regulatory activity that occurs as a response to retinal degeneration.

scholarly journals Comparative effects of Bevacizumab and Ranibizumab on endothelial cells. Implications in age‐related macular degeneration

2009 ◽  
Vol 23 (S1) ◽  
Author(s):  
Raquel Costa ◽  
Ana Pirraco ◽  
Manuel Falcão ◽  
Ângela Carneiro ◽  
Ana Rocha ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Macular Degeneration ◽  
Age Related Macular Degeneration ◽  
Age Related
Sign in / Sign up

Export Citation Format

Share Document