Metabolic Fingerprints of Proliferative Diabetic Retinopathy: An1H-NMR–Based Metabonomic Approach Using Vitreous Humor

2010 ◽  
Vol 51 (9) ◽  
pp. 4416 ◽  
Author(s):  
Ignasi Barba ◽  
Marta Garcia-Ramírez ◽  
Cristina Hernández ◽  
María Angeles Alonso ◽  
Lluis Masmiquel ◽  
...  
Keyword(s):  
Diabetic Retinopathy ◽  
Proliferative Diabetic Retinopathy ◽  
Vitreous Humor

IMMUNOREACTIVE ENDOTHELIN-1 IN THE VITREOUS HUMOR AND EPIRETINAL MEMBRANES OF PATIENTS WITH PROLIFERATIVE DIABETIC RETINOPATHY

Retina ◽  
2007 ◽  
Vol 27 (2) ◽  
pp. 222-235 ◽  
Author(s):  
MANUELA ROLDÁN-PALLARÉS ◽  
RAQUEL ROLLÍN ◽  
JUAN CARLOS MARTÍNEZ-MONTERO ◽  
ARTURO FERNÁNDEZ-CRUZ ◽  
CARMEN BRAVO-LLATA ◽  
...  
Keyword(s):  
Diabetic Retinopathy ◽  
Proliferative Diabetic Retinopathy ◽  
Vitreous Humor ◽  
Epiretinal Membranes ◽  
Endothelin 1

scholarly journals Cytokine Levels in Human Vitreous in Proliferative Diabetic Retinopathy

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1069
Author(s):  
Dean F. Loporchio ◽  
Emily K. Tam ◽  
Jane Cho ◽  
Jaeyoon Chung ◽  
Gyungah R. Jun ◽  
...  
Keyword(s):  
Diabetic Retinopathy ◽  
Proliferative Diabetic Retinopathy ◽  
Vitreous Humor ◽  
Reactive Protein ◽  
Amyloid A ◽  
Intracellular Adhesion Molecule ◽  
Cytokine Levels ◽  
Quantitative Immunoassay ◽  
Current Therapies ◽  
Endothelial Growth Factor

In this study, we compare the vitreous cytokine profile in patients with proliferative diabetic retinopathy (PDR) to that of patients without PDR. The identification of novel cytokines involved in the pathogenesis of PDR provides candidate therapeutic targets that may stand alone or work synergistically with current therapies in the management of diabetic retinopathy. Undiluted vitreous humor specimens were collected from 74 patients undergoing vitrectomy for various vitreoretinal disorders. Quantitative immunoassay was performed for a panel of 36 neuroinflammatory cytokines in each specimen and assessed to identify differences between PDR (n = 35) and non-PDR (n = 39) patients. Levels of interleukin-8 (IL-8), IL-15, IL-16, vascular endothelial growth factor (VEGF), VEGF-D, c-reactive protein (CRP), serum amyloid-A (SAA), and intracellular adhesion molecule-1 (ICAM1) were significantly increased in the vitreous of PDR patients compared to non-PDR patients (p < 0.05). We report novel increases in IL-15 and IL-16, in addition to the expected VEGF, in the human vitreous humor of patients with PDR. Additionally, we confirm the elevation of ICAM-1, VCAM-1, SAA, IL-8 and CRP in the vitreous of patients with PDR, which has previously been described.

scholarly journals Eicosanoid Profiles in the Vitreous Humor of Patients with Proliferative Diabetic Retinopathy

2020 ◽  
Vol 21 (20) ◽  
pp. 7451
Author(s):  
Albert L Lin ◽  
Richard J Roman ◽  
Kathleen A Regan ◽  
Charlotte A Bolch ◽  
Ching-Jygh Chen ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Diabetic Retinopathy ◽  
Proliferative Diabetic Retinopathy ◽  
Vitreous Humor ◽  
Therapeutic Agents ◽  
Complex Interaction ◽  
Uncontrolled Diabetes ◽  
Future Potential ◽  
Acid Metabolites ◽  
Insight Into

Proliferative diabetic retinopathy is a potentially blinding sequela of uncontrolled diabetes that involves a complex interaction of pro-angiogenic and inflammatory pathways. In this study, we compared the levels of pro-angiogenic arachidonic acid-derived mediators in human vitreous humor obtained from eyes with high-risk proliferative diabetic retinopathy versus controls. The results indicated that lipoxygenase and cytochrome P450-derived eicosanoids were elevated (5-HETE, 12-HETE, 20-HETE, and 20-COOH-AA), and there appeared to be no differences in levels measured in eyes with tractional retinal detachments versus those without. These results provide further insight into the pathogenesis of this disease and for the development of future potential therapeutic agents that target arachidonic acid metabolites to treat diabetic retinopathy.

scholarly journals Proteomics of Vitreous Humor Reveals PPARA, RXR, and LXR Are Possible Upstream Regulators of Proliferative Diabetic Retinopathy

2021 ◽  
Vol 8 ◽  
Author(s):  
Siyan Li ◽  
Enzhong Jin ◽  
Xuan Shi ◽  
Yi Cai ◽  
Hui Zhang ◽  
...  
Keyword(s):  
Diabetic Retinopathy ◽  
Proliferative Diabetic Retinopathy ◽  
Enrichment Analysis ◽  
Vitreous Humor ◽  
Gene Set Enrichment Analysis ◽  
Coagulation System ◽  
Proteomics Data ◽  
Gene Set Enrichment ◽  
Gene Set ◽  
Upstream Regulators

Purpose: To investigate the key regulators of the disease by comparing the abundance of vitreous proteins between the patients with proliferative diabetic retinopathy (PDR) and the controls with idiopathic epiretinal membrane (iERM).Methods: Vitreous humor (VH) samples were derived from patients with PDR or iERM through the pars plana vitrectomy. The VH proteins were identified by liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis. MaxQuant software and Metascape were applied to explore the enrichment of differentially expressed proteins in biological processes, cellular components, and molecular functions. Enrichr online tool and Gene Set Enrichment Analysis (GSEA) were performed to detect upstream transcriptional regulators of the highly expressed proteins.Results: The present study collected 8 vitreous humor samples from 5 PDR eyes and 3 iERM eyes and identified 88 highly expressed proteins in PDR patients. We validated our highly expressed proteome was able to distinguish the PDR patients from the non-PDR patients by using the VH proteomics data from a previous study. The majority of highly expressed proteins were involved in complement and coagulation cascades, regulating exocytosis, and hemostasis. Using the Gene Set Enrichment Analysis (GSEA), we identified that transcription factors (TFs) PPAR-α, RXR, LXR regulate these proteins.Conclusions: In this study, we identified a highly expressed proteome in VH of PDR patients. The role of the complement and coagulation system, regulating exocytosis, and hemostasis has been of great significance to PDR. Nuclear receptors PPARA, RXR, LXR were possible upstream regulators of disease progression and required further study.

MicroRNA-200b Expression in the Vitreous Humor of Patients with Proliferative Diabetic Retinopathy

2017 ◽  
Vol 58 (3) ◽  
pp. 168-175 ◽  
Author(s):  
Amir Ramadan Gomaa ◽  
Eman Tayae Elsayed ◽  
Reham Fadl Moftah
Keyword(s):  
Diabetic Retinopathy ◽  
Proliferative Diabetic Retinopathy ◽  
Vitreous Humor

scholarly journals Characterization of Vitreous and Aqueous Proteome in Humans With Proliferative Diabetic Retinopathy and Its Clinical Correlation

2017 ◽  
Vol 8 ◽  
pp. 117864181668607 ◽  
Author(s):  
Sankarathi Balaiya ◽  
Zimei Zhou ◽  
Kakarla V Chalam
Keyword(s):  
Mass Spectrometry ◽  
Diabetic Retinopathy ◽  
Proliferative Diabetic Retinopathy ◽  
Inflammatory Responses ◽  
Microvascular Complications ◽  
Vitreous Humor ◽  
Human Retina ◽  
Liquid Chromatography Mass Spectrometry ◽  
Mass Spectrometry Mass Spectrometry

Aims: Proliferative diabetic retinopathy (PDR) is associated with microvascular complications that cause biochemical changes in the human retina and alter the proteome of vitreous humor and aqueous humor (AH). Methods: Human vitreous humor and AH of PDR subjects were collected. Subjects who had surgery for epiretinal membrane or macular hole served as controls. Protein profiles were obtained and analyzed after running the samples on a liquid chromatography-mass spectrometry/mass spectrometry. Results: In vitreous humor, 16 unique proteins were noted in PDR patients, but not in controls. Those were associated mainly with coagulation, complement, and kallikrein-kinin systems. Under coagulation, fibrinogen and prothrombin proteins were more evident and may emphasize the importance of angiogenesis in the development of PDR. Vitreous proteins showed replicative presence in AH too. As for AH samples, we detected 10 proteins found in PDR patients, which were related to transport, coagulation, and inflammatory responses. Conclusions: We found 57 proteins in human vitreous and 39 proteins in AH. Identification of these proteins that are involved in various pathways will be helpful to understand diabetic retinopathy pathogenesis and to develop proteome as a biomarker for PDR.

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