scholarly journals Gene Expression Changes in Areas of Focal Loss of Retinal Ganglion Cells in the Retina of DBA/2J Mice

2010 ◽  
Vol 51 (4) ◽  
pp. 2024 ◽  
Author(s):  
Lampros Panagis ◽  
Xiujun Zhao ◽  
Yongchao Ge ◽  
Lizhen Ren ◽  
Thomas W. Mittag ◽  
...  
Keyword(s):  
Gene Expression ◽  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Retinal Ganglion

scholarly journals Endothelin-Mediated Changes in Gene Expression in Isolated Purified Rat Retinal Ganglion Cells

2015 ◽  
Vol 56 (10) ◽  
pp. 6144 ◽  
Author(s):  
Shaoqing He ◽  
Yong H. Park ◽  
Thomas Yorio ◽  
Raghu R. Krishnamoorthy
Keyword(s):  
Gene Expression ◽  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Retinal Ganglion

scholarly journals Retinal Ganglion Cells Downregulate Gene Expression and Lose Their Axons within the Optic Nerve Head in a Mouse Glaucoma Model

2008 ◽  
Vol 28 (2) ◽  
pp. 548-561 ◽  
Author(s):  
I. Soto ◽  
E. Oglesby ◽  
B. P. Buckingham ◽  
J. L. Son ◽  
E. D. O. Roberson ◽  
...  
Keyword(s):  
Gene Expression ◽  
Optic Nerve ◽  
Retinal Ganglion Cells ◽  
Optic Nerve Head ◽  
Ganglion Cells ◽  
Retinal Ganglion

A System for Inducible Gene Expression in Retinal Ganglion Cells

2005 ◽  
Vol 46 (8) ◽  
pp. 2932 ◽  
Author(s):  
John B. Kerrison ◽  
Elia J. Duh ◽  
Yilin Yu ◽  
Deborah C. Otteson ◽  
Donald J. Zack
Keyword(s):  
Gene Expression ◽  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Retinal Ganglion ◽  
Inducible Gene Expression ◽  
Inducible Gene

scholarly journals Mouse γ-Synuclein Promoter-Mediated Gene Expression and Editing in Mammalian Retinal Ganglion Cells

2020 ◽  
Vol 40 (20) ◽  
pp. 3896-3914 ◽  
Author(s):  
Qizhao Wang ◽  
Pei Zhuang ◽  
Haoliang Huang ◽  
Liang Li ◽  
Liang Liu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Retinal Ganglion

scholarly journals Single Cell Sequencing of Induced Pluripotent Stem Cell Derived Retinal Ganglion Cells (iPSC-RGC) Reveals Distinct Molecular Signatures and RGC Subtypes

Genes ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 2015
Author(s):  
Harini V. Gudiseva ◽  
Vrathasha Vrathasha ◽  
Jie He ◽  
Devesh Bungatavula ◽  
Joan M. O’Brien ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cell ◽  
Single Cell ◽  
Retinal Ganglion Cells ◽  
Pluripotent Stem Cell ◽  
Ganglion Cells ◽  
Induced Pluripotent Stem Cell ◽  
Retinal Ganglion ◽  
Single Cell Sequencing ◽  
Induced Pluripotent

We intend to identify marker genes with differential gene expression (DEG) and RGC subtypes in cultures of human-induced pluripotent stem cell (iPSC)-derived retinal ganglion cells. Single-cell sequencing was performed on mature and functional iPSC-RGCs at day 40 using Chromium Single Cell 3’ V3 protocols (10X Genomics). Sequencing libraries were run on Illumina Novaseq to generate 150 PE reads. Demultiplexed FASTQ files were mapped to the hg38 reference genome using the STAR package, and cluster analyses were performed using a cell ranger and BBrowser2 software. QC analysis was performed by removing the reads corresponding to ribosomal and mitochondrial genes, as well as cells that had less than 1X mean absolute deviation (MAD), resulting in 4705 cells that were used for further analyses. Cells were separated into clusters based on the gene expression normalization via PCA and TSNE analyses using the Seurat tool and/or Louvain clustering when using BBrowser2 software. DEG analysis identified subsets of RGCs with markers like MAP2, RBPMS, TUJ1, BRN3A, SOX4, TUBB3, SNCG, PAX6 and NRN1 in iPSC-RGCs. Differential expression analysis between separate clusters identified significant DEG transcripts associated with cell cycle, neuron regulatory networks, protein kinases, calcium signaling, growth factor hormones, and homeobox transcription factors. Further cluster refinement identified RGC diversity and subtype specification within iPSC-RGCs. DEGs can be used as biomarkers for RGC subtype classification, which will allow screening model systems that represent a spectrum of diseases with RGC pathology.

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