Primary Culture of Human Blood–Retinal Barrier Cells and Preliminary Study of APOBEC3 Expression: An In Vitro Study

2009 ◽  
Vol 50 (9) ◽  
pp. 4436 ◽  
Author(s):  
Haotian Lin ◽  
Zhenping Zhang ◽  
Hui Zhang ◽  
Pisong Yan ◽  
Qilin Wang ◽  
...  
Keyword(s):  
Primary Culture ◽  
Human Blood ◽  
In Vitro Study ◽  
Vitro Study ◽  
Blood Retinal Barrier ◽  
Preliminary Study ◽  
Apobec3 Expression

scholarly journals Non Invasive Detection of Dengue Viruses from Saliva: In vitro Study

2018 ◽  
Vol 22 (2) ◽  
pp. 75
Author(s):  
Budi Saksono
Keyword(s):  
Dengue Virus ◽  
Detection System ◽  
Point Of Care ◽  
Virus Detection ◽  
In Vitro Study ◽  
Vitro Study ◽  
Dengue Viruses ◽  
Non Invasive ◽  
Preliminary Study

      In the previous paper, we had succeeded in developing an early detection system of dengue viruses using Sugar liganded Gold Nano Particle (SGNP) only from 6 μL serum. It has been reported that dengue virus is also detected in the saliva and urine of the patient. The evidences lead to the possibility of developing non-invasive methods of dengue virus detection. In this in vitro study, we evaluated the utility of SGNP to capture and concentrate dengue virion in 10% saliva solution. The results showed that dengue virion was successfully detected in 10% of saliva solution. Analysis of virion stability during storage showed that virions in salivary samples were stable up to 3 days at temperature wherease the RNA has significantly degraded. Although still a preliminary study, the data obtained show the prospect of SGNP as a non-invasive dengue virus detection method, as well as the development of POC (Point of Care) method. Clinical trials using saliva from dengue viruses infected patients need to be done to prove the effectiveness of the SGNP method.

Stability of Fibrinogen Concentrate in Human Blood Samples: An In Vitro Study

2018 ◽  
Vol 183 (suppl_1) ◽  
pp. 183-188 ◽  
Author(s):  
Wenjun Z Martini ◽  
Rodolfo de Guzman ◽  
Michael A Dubick
Keyword(s):  
Human Blood ◽  
In Vitro Study ◽  
Vitro Study ◽  
Fibrinogen Concentrate ◽  
Blood Samples ◽  
Human Blood Samples
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