Decreased Visual Function after Patchy Loss of Retinal Pigment Epithelium Induced by Low-Dose Sodium Iodate

2009 ◽  
Vol 50 (8) ◽  
pp. 4004 ◽  
Author(s):  
Luisa M. Franco ◽  
Rahel Zulliger ◽  
Ute E. K. Wolf-Schnurrbusch ◽  
Yoshiaki Katagiri ◽  
Henry J. Kaplan ◽  
...  
Keyword(s):  
Retinal Pigment Epithelium ◽  
Visual Function ◽  
Low Dose ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Sodium Iodate

Endogenous regeneration of damaged retinal pigment epithelium following low dose sodium iodate administration: An insight into the role of glial cells in retinal repair

2013 ◽  
Vol 112 ◽  
pp. 68-78 ◽  
Author(s):  
Anna Machalińska ◽  
Miłosz Piotr Kawa ◽  
Ewa Pius-Sadowska ◽  
Dorota Rogińska ◽  
Patrycja Kłos ◽  
...  
Keyword(s):  
Retinal Pigment Epithelium ◽  
Glial Cells ◽  
Low Dose ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Sodium Iodate ◽  
Insight Into

scholarly journals Fine Tuning of an Oxidative Stress Model with Sodium Iodate Revealed Protective Effect of NF-κB Inhibition and Sex-Specific Difference in Susceptibility of the Retinal Pigment Epithelium

Antioxidants ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 103
Author(s):  
Xue Yang ◽  
Usha Rai ◽  
Jin-Yong Chung ◽  
Noriko Esumi
Keyword(s):  
Oxidative Stress ◽  
Retinal Pigment Epithelium ◽  
Low Dose ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Fine Tuning ◽  
Dose Effect ◽  
High Dose ◽  
Sodium Iodate ◽  
Quantification Method

Oxidative stress of the retinal pigment epithelium (RPE) is a major risk factor for age-related macular degeneration (AMD). As a dry AMD model via oxidative stress, sodium iodate (NaIO3), which is primarily toxic to the RPE, has often been used at a high dose to cause RPE death for studying photoreceptor degeneration. Thus, characterization of RPE damage by a low dose of NaIO3 is still limited. To quantify RPE damage caused by NaIO3 in mice, we recently developed a morphometric method using RPE flat-mounts. Here, we report that NaIO3 has a narrow range of dose–effect correlation at 11–18 mg/kg body weight in male C57BL/6J mice. We evaluated the usefulness of our quantification method in two experimental settings. First, we tested the effect of NF-κB inhibition on NaIO3-induced RPE damage in male C57BL/6J mice. IKKβ inhibitor BAY 651942 suppressed upregulation of NF-κB targets and protected the RPE from oxidative stress. Second, we tested sex-specific differences in NaIO3-induced RPE damage in C57BL/6J mice using a low dose near the threshold. NaIO3 caused more severe RPE damage in female mice than in male mice. These results demonstrate the usefulness of the quantification method and the importance of fine-tuning of the NaIO3 dose. The results also show the therapeutic potential of IKKβ inhibition for oxidative stress-related RPE diseases, and reveal previously-unrecognized sex-specific differences in RPE susceptibility to oxidative stress.

High-voltage electron microscopy of subretinal scar formation

1992 ◽  
Vol 50 (1) ◽  
pp. 486-487
Author(s):  
G.E. Korte ◽  
M. Marko ◽  
G. Hageman
Keyword(s):  
Electron Microscopy ◽  
Monoclonal Antibody ◽  
Retinal Pigment Epithelium ◽  
Glial Cells ◽  
High Voltage ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Sodium Iodate ◽  
High Voltage Electron Microscopy ◽  
Voltage Electron

Sodium iodate iv. damages the retinal pigment epithelium (RPE) in rabbits. Where RPE does not regenerate (e.g., 1,2) Muller glial cells (MC) forma subretinal scar that replaces RPE. The MC response was studied by HVEM in 3D computer reconstructions of serial thick sections, made using the STEREC0N program (3), and the HVEM at the NYS Dept. of Health in Albany, NY. Tissue was processed for HVEM or immunofluorescence localization of a monoclonal antibody recognizing MG microvilli (4).

scholarly journals Electrophysiologic and Morphologic Strain Differences in a Low-Dose NaIO3-Induced Retinal Pigment Epithelium Damage Model

2021 ◽  
Vol 10 (8) ◽  
pp. 10
Author(s):  
Nan Zhang ◽  
Xian Zhang ◽  
Preston E. Girardot ◽  
Micah A. Chrenek ◽  
Jana T. Sellers ◽  
...  
Keyword(s):  
Retinal Pigment Epithelium ◽  
Low Dose ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Damage Model ◽  
Strain Differences

scholarly journals Transplantation of retinal pigment epithelium and photoreceptors generated concomitantly via small molecule-mediated differentiation rescues visual function in rodent models of retinal degeneration

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Harshini Surendran ◽  
Swapna Nandakumar ◽  
Vijay Bhaskar Reddy K ◽  
Jonathan Stoddard ◽  
Varsha Mohan K ◽  
...  
Keyword(s):  
Retinal Pigment Epithelium ◽  
Visual Function ◽  
De Novo ◽  
Pigment Epithelium ◽  
Outer Nuclear Layer ◽  
Retinal Pigment ◽  
Safety Data ◽  
Age Related Macular Degeneration ◽  
Age Related ◽  
Promising Therapeutic Approach

Abstract Background Age-related macular degeneration (AMD) is a result of degeneration/damage of the retinal pigment epithelium (RPE) while retinitis pigmentosa (RP), an inherited early-onset disease, results from premature loss of photoreceptors. A promising therapeutic approach for both is the replacement of lost/damaged cells with human induced pluripotent stem cell (hiPSC)-derived retinal cells. Methods The aim of this study was to investigate the in vivo functionality of RPE and photoreceptor progenitor (PRP) cells derived from a clinical-grade hiPSC line through a unified protocol. De novo-generated RPE and PRP were characterized extensively to validate their identity, purity, and potency. Results RPE expressed tight junction proteins, showed pigmentation and ciliation, and secreted polarization-related factors vascular endothelial growth factor (VEGF) and pigment epithelium-derived factor (PEDF). PRP expressed neural retina proteins and cone and rod markers, and responded to KCl-induced polarization. Transcriptomic analysis demonstrated an increase in the expression of mature retinal tissue-specific genes coupled with concomitant downregulation of genes from undesired lineages. RPE transplantation rescued visual function in RCS rats shown via optokinetic tracking and photoreceptor rescue. PRP transplantation improved light perception in NOD.SCID-rd1 mice, and positive electroretinography signals indicated functional photoreceptor activity in the host’s outer nuclear layer. Graft survival and integration were confirmed using immunohistochemistry, and no animals showed teratoma formation or any kind of ectopic growth in the eye. Conclusions To our knowledge, this is the first demonstration of a unified, scalable, and GMP-adaptable protocol indicating strong animal efficacy and safety data with hiPSC-derived RPE and PRP cells. These findings provide robust proof-of-principle results for IND-enabling studies to test these potential regenerative cell therapies in patients.

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