Cross-Linked Actin Networks (CLANs) in the Trabecular Meshwork of the Normal and Glaucomatous Human Eye In Situ

2009 ◽  
Vol 50 (3) ◽  
pp. 1255 ◽  
Author(s):  
Mary-Jo Hoare ◽  
Ian Grierson ◽  
Daniel Brotchie ◽  
Natalie Pollock ◽  
Kathy Cracknell ◽  
...  
Keyword(s):  
Trabecular Meshwork ◽  
Actin Networks ◽  
Human Eye

scholarly journals Functional Implications of Cross-Linked Actin Networks in Trabecular Meshwork Cells

2018 ◽  
Vol 45 (2) ◽  
pp. 783-794 ◽  
Author(s):  
Laura Duffy ◽  
Steven O’Reilly
Keyword(s):  
Trabecular Meshwork ◽  
Open Angle Glaucoma ◽  
Collagen Gel ◽  
Mechanical Stretch ◽  
Actin Networks ◽  
Trabecular Meshwork Cells ◽  
Microscopy Technique ◽  
Collagen Gel Contraction

Background/Aims: The Trabecular meshwork (TM) is the tissue responsible for outflow resistance and therefore intraocular pressure. TM cells contain a contractile apparatus that is composed of actin stress fibres which run parallel to the axis of the cell and are responsible for facilitating contraction. Cross-Linked Actin Networks (CLANs) are polygonal arrangements of actin that form a geodesic network found predominantly in TM cells both in situ and in vitro. The aim of this work is to determine the functional significance of CLANs in TM cells and to assess the effect of mechanical stretch stimulation on the induction (or not) of CLANs. Methods: We used collagen gel contraction models to demonstrate functional impairment of cells when induced to express CLANs in situ. Cyclic mechanical stretch was used to stimulate cells and measure CLANs Results: CLANs inhibited contraction and cyclic mechanical stretch induced CLANs. Furthermore, we also demonstrated that using shape alone we could predict the appearance of CLANs using a simple light microscopy technique. Conclusion: Taken together we have now shown, for the first time, a functional deficit In TM cells with CLANs Furthermore that shape alone can predict the appearance of CLAN containing cells. CLANs can now be linked to a functional effect and may underlie the appearance of CLANs with the pathology of primary open angle glaucoma (POAG).

Circumferential trabecular meshwork cell density in the human eye

2021 ◽  
Vol 205 ◽  
pp. 108494
Author(s):  
Markus H. Kuehn ◽  
Janice A. Vranka ◽  
David Wadkins ◽  
Thomas Jackson ◽  
Lin Cheng ◽  
...  
Keyword(s):  
Cell Density ◽  
Trabecular Meshwork ◽  
Human Eye ◽  
Trabecular Meshwork Cell

scholarly journals In Situ Autofluorescence Visualization of Human Trabecular Meshwork Structure

2012 ◽  
Vol 53 (4) ◽  
pp. 2080 ◽  
Author(s):  
James C. H. Tan ◽  
Jose M. Gonzalez ◽  
Sarah Hamm-Alvarez ◽  
Jonathan Song
Keyword(s):  
Trabecular Meshwork

scholarly journals Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye

10.3791/52611 ◽  
2015 ◽  
Author(s):  
Larry Kagemann ◽  
Bo Wang ◽  
Gadi Wollstein ◽  
Hiroshi Ishikawa ◽  
Brandon Mentley ◽  
...  
Keyword(s):  
Trabecular Meshwork ◽  
Human Eye ◽  
Pressure Elevation

Cross-linked actin networks (CLANs) in bovine trabecular meshwork cells

2009 ◽  
Vol 89 (5) ◽  
pp. 648-659 ◽  
Author(s):  
N.C. Wade ◽  
I. Grierson ◽  
S. O'Reilly ◽  
M.J. Hoare ◽  
K.P.B. Cracknell ◽  
...  
Keyword(s):  
Trabecular Meshwork ◽  
Actin Networks ◽  
Trabecular Meshwork Cells

scholarly journals BLUE LIGHT AND THE REGENERATION OF HUMAN RHODOPSIN IN SITU

1957 ◽  
Vol 41 (2) ◽  
pp. 419-428 ◽  
Author(s):  
W. A. H. Rushton
Keyword(s):  
Blue Light ◽  
Yellow Light ◽  
Human Eye ◽  
Equilibrium Level ◽  
Rapid Regeneration ◽  
Fundus Oculi

Hubbard has found that the photoisomerization of retinene was important for the regeneration of rhodopsin in vitro, and the object of the present investigation was to find whether this was also true for regeneration in the living human eye. In the Appendix is described a device which permits the rhodopsin density to be measured by analysing the light reflected from the fundus oculi in an ophthalmoscopic arrangement, the measurement taking about 5 seconds. Now if a blue and a yellow light viewed scotopically are adjusted in intensity so as to appear identical, they must bleach rhodopsin equally, but the blue will be more than 10 times as effective in isomerizing retinene. Therefore if retinene isomerization is important for rhodopsin regeneration, blue light should cause a more rapid regeneration after bleaching, and during bleaching the equilibrium level attained should be less profound. But, as the figures show, the course of bleaching and regeneration is identical for the matched yellow or blue bleaching lights, therefore isomerization of retinene is not important for rhodopsin regeneration in the living human eye.

scholarly journals Two-Photon Immunofluorescence Characterization of the Trabecular Meshwork In Situ

2012 ◽  
Vol 53 (7) ◽  
pp. 3395 ◽  
Author(s):  
Jose M. Gonzalez ◽  
Martin Heur ◽  
James C. H. Tan
Keyword(s):  
Trabecular Meshwork ◽  
Two Photon

Expression of the TIGR gene in the iris, ciliary body, and trabecular meshwork of the human eye

2000 ◽  
Vol 21 (3) ◽  
pp. 155-169 ◽  
Author(s):  
Wenhu Huang ◽  
Jan Jaroszewski ◽  
Javier Ortego ◽  
Julio Escribano ◽  
Miguel Coca-Prados
Keyword(s):  
Trabecular Meshwork ◽  
Ciliary Body ◽  
Human Eye
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