scholarly journals Cyclic Mechanical Stress and Trabecular Meshwork Cell Contractility

2009 ◽  
Vol 50 (8) ◽  
pp. 3826 ◽  
Author(s):  
Renata F. Ramos ◽  
Grant M. Sumida ◽  
W. Daniel Stamer
Keyword(s):  
Mechanical Stress ◽  
Trabecular Meshwork ◽  
Cell Contractility ◽  
Trabecular Meshwork Cell

Regulation of Endothelin-1–Induced Trabecular Meshwork Cell Contractility by Latanoprostene Bunod

2015 ◽  
Vol 56 (6) ◽  
pp. 4108 ◽  
Author(s):  
Megan E. Cavet ◽  
Thomas R. Vollmer ◽  
Karen L. Harrington ◽  
Karl VanDerMeid ◽  
Mary E. Richardson
Keyword(s):  
Trabecular Meshwork ◽  
Cell Contractility ◽  
Endothelin 1 ◽  
Trabecular Meshwork Cell

Circumferential trabecular meshwork cell density in the human eye

2021 ◽  
Vol 205 ◽  
pp. 108494
Author(s):  
Markus H. Kuehn ◽  
Janice A. Vranka ◽  
David Wadkins ◽  
Thomas Jackson ◽  
Lin Cheng ◽  
...  
Keyword(s):  
Cell Density ◽  
Trabecular Meshwork ◽  
Human Eye ◽  
Trabecular Meshwork Cell

Mechanical strain increases velocity and extent of shortening in cultured airway smooth muscle cells

1999 ◽  
Vol 277 (2) ◽  
pp. L343-L348 ◽  
Author(s):  
Paul G. Smith ◽  
Chaity Roy ◽  
Jamie Dreger ◽  
Frank Brozovich
Keyword(s):  
Smooth Muscle ◽  
Mechanical Stress ◽  
Airway Smooth Muscle ◽  
Light Chain ◽  
Myosin Light Chain ◽  
Mechanical Strain ◽  
Airway Smooth Muscle Cells ◽  
Maximal Velocity ◽  
Cell Contractility ◽  
Cell Shortening

Abnormal mechanical stress on lung tissue is associated with increased mass and contractility of airway smooth muscle (ASM). We have reported that cultured ASM cells subjected to cyclic strain exhibit increased myosin light chain kinase (MLCK) and stress filaments. Increased MLCK may increase contractile velocity, whereas increased stress filaments could impede cell shortening by increasing the cell’s internal load. To study strain-induced changes in cell contractility, the time course of shortening of individual cells exposed to 90 mM KCl was recorded. Length vs. time plots revealed significantly greater maximal velocity of shortening in strain cells than control (no strain). This correlated with an increase in MLCK and myosin light chain phosphorylation measured in strain cells in separate experiments. The extent of cell shortening tended to be greater in the strain cells so that increased impedance to shortening was not detected. Mechanical stress may therefore increase the contractility of ASM by increasing the content of MLCK.

scholarly journals Syndecan-4 Promotes Epithelial Tumor Cells Spreading and Regulates the Turnover of PKCα Activity under Mechanical Stimulation on the Elastomeric Substrates

2015 ◽  
Vol 36 (4) ◽  
pp. 1291-1304 ◽  
Author(s):  
Chun-Ping Huang ◽  
Chao-Min Cheng ◽  
Hong-Lin Su ◽  
Yi-Wen Lin
Keyword(s):  
Mechanical Stress ◽  
Tumor Cells ◽  
Focal Adhesion ◽  
Mechanical Stimulation ◽  
Binding Motif ◽  
Epithelial Tumor ◽  
Cell Contractility ◽  
Myosin Light Chain 2 ◽  
Epithelial Tumor Cells ◽  
Time Courses

Background: Heparan sulfate proteoglycans (HSPGs) at the cell surface play an important role in cell adhesion, spreading, formation of focal adhesion complexes (FACs), and sensing mechanical stress. Syndecans are members of the HSPGs family and are highly expressed in various tumor cells. Syndecan-4 (SDC4) is a unique member of syndecans that activates protein kinase C alpha (PKCα). However, syndecan-4 in tumor cells development is not clear when receiving mechanical stress. Aims: Here we investigate the role of syndecan-4 in tumor cells spreading and its downstream kinases under mechanical stimulation. Methods: Epithelial tumor cells were seeded onto elastomeric polydimethylsiloxane (PDMS) membranes coated with poly-L-lysine (Pl), fibronectin (Fn), or anti-SDC4 antibody and stretched with a modified pressure-driven cell-stretching (PreCS) device. Results: When cells received mechanical stimulation, engagement of syndecan-4 promoted the phosphorylation of focal adhesion kinase (FAK) at tyrosine 397 and PKCα at serine 657. Furthermore, we analyzed the cell contractility marker—myosin light chain 2 (MLC2) in 30 min time courses. The levels of phosphorylated MLC2 at serine19 were augmented through ligations of syndecan-4 but not integrin binding motif (RGD) at 10 min mechanical stimulation and were suppressed at 30 min and this phenomenon was associated with the activity of PKCα. Conclusion: Our data demonstrate that syndecan-4 is essential for transmitting the mechanotransduction signals via activation of PKCα and is important for tumor cells spreading, assembly of actin cytoskeleton and cell contractility.

Caveolin‐1 regulates human trabecular meshwork cell adhesion, endocytosis, and autophagy

2019 ◽  
Vol 120 (8) ◽  
pp. 13382-13391 ◽  
Author(s):  
Zhenggen Wu ◽  
Chukai Huang ◽  
Ciyan Xu ◽  
Lijing Xie ◽  
Jia‐Jian Liang ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Trabecular Meshwork ◽  
Caveolin 1 ◽  
Trabecular Meshwork Cell

scholarly journals The many faces of the trabecular meshwork cell

2017 ◽  
Vol 158 ◽  
pp. 112-123 ◽  
Author(s):  
W. Daniel Stamer ◽  
Abbot F. Clark
Keyword(s):  
Trabecular Meshwork ◽  
Trabecular Meshwork Cell ◽  
The Many
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