scholarly journals Characterization and Comparison of Intercellular Adherent Junctions Expressed by Human Corneal Endothelial Cells In Vivo and In Vitro

2008 ◽  
Vol 49 (9) ◽  
pp. 3879 ◽  
Author(s):  
Ying-Ting Zhu ◽  
Yasutaka Hayashida ◽  
Ahmad Kheirkhah ◽  
Hua He ◽  
Szu-Yu Chen ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Corneal Endothelial Cells ◽  
Human Corneal Endothelial Cells

scholarly journals Extracellular Vesicles Derived From Human Corneal Endothelial Cells Inhibit Proliferation of Human Corneal Endothelial Cells

2021 ◽  
Author(s):  
Mohit Parekh ◽  
Hefin Rhys ◽  
Tiago Ramos ◽  
Stefano Ferrari ◽  
Sajjad Ahmad
Keyword(s):  
Endothelial Cells ◽  
Extracellular Vesicles ◽  
Ex Vivo ◽  
Treatment Options ◽  
Proliferative Capacity ◽  
Corneal Endothelial Cells ◽  
Human Corneal Endothelial Cells ◽  
The Media

Abstract Corneal endothelial cells (CEnCs) are a monolayer of hexagonal cells that are responsible for maintaining the function and transparency of the cornea. Damage or dysfunction of CEnCs could lead to blindness. Human CEnCs (HCEnCs) have shown limited proliferative capacity in vivo hence, their maintenance is crucial. Extracellular vesicles (EVs), are responsible for inter- and intra-cellular communication, proliferation, cell-differentiation, migration, and many other complex biological processes. Therefore, we investigated the effect of EVs (derived from human corneal endothelial cell line – HCEC-12) on corneal endothelial cells. HCEC-12 cells were starved with serum-depleted media for 72 hours. The media was ultracentrifuged at 100,000xg to isolate the EVs. EV counting, characterization, internalization and localization were performed using NanoSight, flow cytometry, Dil labelling and confocal microscopy respectively. HCEC-12 and HCEnCs were cultured with media supplemented with EVs. Extracted EVs showed a homogeneous mixture of exosomes and microvesicles. Cells with EVs decreased the proliferation rate; increased apoptosis and cell size; showed poor wound healing response in vitro and on ex vivo human, porcine, and rabbit CECs. Thirteen miRNAs were found in the EV sample using next generation sequencing. We observed that increased cellular uptake of EVs by CECs limit the proliferative capacity of HCEnCs. These preliminary data may help in understanding the pathology of corneal endothelial dysfunction and provide further insights in the development of future therapeutic treatment options.

scholarly journals Human Corneal Endothelial Cells Expanded In Vitro Are a Powerful Resource for Tissue Engineering

2017 ◽  
Vol 14 (2) ◽  
pp. 128-135 ◽  
Author(s):  
Yongsong Liu ◽  
Hong Sun ◽  
Min Hu ◽  
Min Zhu ◽  
Sean Tighe ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Endothelial Cells ◽  
Corneal Endothelial Cells ◽  
Human Corneal Endothelial Cells

Role of Human Corneal Endothelial Cells in T-Cell–Mediated Alloimmune Attack In Vitro

2014 ◽  
Vol 55 (3) ◽  
pp. 1213 ◽  
Author(s):  
Imad Lahdou ◽  
Christoph Engler ◽  
Stefan Mehrle ◽  
Volker Daniel ◽  
Mahmoud Sadeghi ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
T Cell ◽  
Corneal Endothelial Cells ◽  
Human Corneal Endothelial Cells

Laminin-511 and -521 Enable Efficient In Vitro Expansion of Human Corneal Endothelial Cells

2015 ◽  
Vol 56 (5) ◽  
pp. 2933 ◽  
Author(s):  
Naoki Okumura ◽  
Kazuya Kakutani ◽  
Ryohei Numata ◽  
Makiko Nakahara ◽  
Ursula Schlötzer-Schrehardt ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Corneal Endothelial Cells ◽  
Human Corneal Endothelial Cells ◽  
In Vitro Expansion

scholarly journals Engineering of Human Corneal Endothelial Cells In Vitro

2019 ◽  
Vol 16 (4) ◽  
pp. 507-512
Author(s):  
Qin Zhu ◽  
Yingting Zhu ◽  
Sean Tighe ◽  
Yongsong Liu ◽  
Min Hu
Keyword(s):  
Endothelial Cells ◽  
Corneal Endothelial Cells ◽  
Human Corneal Endothelial Cells

scholarly journals Cytotoxicity of proparacaine to human corneal endothelial cells in vitro

2015 ◽  
Vol 40 (4) ◽  
pp. 427-436 ◽  
Author(s):  
Qian Wen ◽  
Tingjun Fan ◽  
Suran Bai ◽  
Yunlong Sui
Keyword(s):  
Endothelial Cells ◽  
Corneal Endothelial Cells ◽  
Human Corneal Endothelial Cells

scholarly journals Persimmon Leaves (Diospyros kaki) Extract Enhances the Viability of Human Corneal Endothelial Cells by Improving Na+-K+-ATPase Activity

2022 ◽  
Vol 15 (1) ◽  
pp. 72
Author(s):  
Ramsha Afzal ◽  
Hyung Bin Hwang
Keyword(s):  
Endothelial Cells ◽  
Atpase Activity ◽  
Basolateral Membrane ◽  
Corneal Edema ◽  
Ethanol Extract ◽  
Diospyros Kaki ◽  
Corneal Endothelial Cells ◽  
Human Corneal Endothelial Cells ◽  
Corneal Decompensation

The Na+/K+-ATPase, present in the basolateral membrane of human corneal endothelial cells (HCECs), is known to play an important role for corneal transparency. Na+/K+-ATPase dysfunction is one of the major causes of corneal decompensation. The ethanol extract of Diospyros kaki (EEDK) has been reported to increase corneal cell viability. Thus, we treated HCECs with EEDK and studied its effects on HCECs survival and Na+/K+-ATPase against cytotoxic drugs like staurosporine (ST) and ouabain (OU). Firstly, survival assays, (MTT assay and live dead-imaging) showed that decreased HCECs viability by ST and OU was significantly recovered by EEDK co-treatment. Secondly, Na+/K+-ATPase activity assays revealed that EEDK enhanced Na+/K+-ATPase enzymatic activity (* p < 0.01) with/without ST and OU. Finally, Na+/K+-ATPase expression analysis (Western Blot and confocal microscopy) demonstrated that EEDK treatment with/without ST and OU facilitates Na+/K+-ATPase expression in HCECs. Taken together, our findings led us to the conclusion that EEDK might aid HCECs survival in vitro by increasing the activity and expression of Na+/K+-ATPase enzyme. Since Na+/K+-ATPase activity is important to maintain cellular function of HCECs, we suggest that EEDK can be a potential effective agent against corneal edema and related corneal disorders.

scholarly journals Injury-Free In Vivo Delivery and Engraftment into the Cornea Endothelium Using Extracellular Matrix Shrink-Wrapped Cells

2021 ◽  
Author(s):  
Rachelle N. Palchesko ◽  
Yiqin Du ◽  
Moira L. Geary ◽  
Santiago Carrasquilla ◽  
Daniel J. Shiwarski ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Single Cell ◽  
Single Cells ◽  
Tissue Level ◽  
Cell Junctions ◽  
Small Island ◽  
Corneal Endothelial Cells ◽  
Corneal Blindness

AbstractCell injection has emerged as a widespread approach for therapeutic delivery of healthy cells into diseased and damaged tissues to achieve regeneration. However, cell retention, viability and integration at the injection site has generally been poor, driving the need for improved approaches. Additionally, it is unknown how efficiently single cells can integrate and repair tissue level function. Here we have developed a technique to address these issues by engineering islands of interconnected cells on ECM nanoscaffolds that can be non-destructively released from the surface via thermal dissolution of the underlying thermo-responsive polymer. Upon dissolution of the polymer, the ECM nanoscaffold shrink-wraps around the small island of cells, creating a small patch of cells that maintain their cell-cell junctions and cytoskeletal structure throughout collection, centrifugation and injection that we have termed μMonolayers. These μMonolayers were made with corneal endothelial cells, as a model system, as single cell injections of corneal endothelial cells have been used with some success clinically to treat corneal blindness. In vitro our μMonolayers exhibited increased integration compared to single cells into low density corneal endothelial monolayers and in vivo into the high-density healthy rabbit corneal endothelium. These results indicate that this technique could be used to increase the integration of healthy cells into existing tissues to treat not only corneal blindness, but also other conditions such as cystic fibrosis, myocardial infarction, diabetes, etc.One Sentence SummarySmall monolayers of interconnected endothelial cells are shrinkwrapped in a thin layer of ECM and exhibit enhanced adhesion and integration in vivo compared to single cell suspensions.

Effect of amniotic fluid on the in vitro culture of human corneal endothelial cells

2014 ◽  
Vol 122 ◽  
pp. 132-140 ◽  
Author(s):  
Sepehr Feizi ◽  
Zahra-Soheila Soheili ◽  
Abouzar Bagheri ◽  
Sahar Balagholi ◽  
Azam Mohammadian ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Amniotic Fluid ◽  
In Vitro Culture ◽  
Corneal Endothelial Cells ◽  
Human Corneal Endothelial Cells
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