Effect of Amniotic Membrane Transplantation on the Healing of Bacterial Keratitis

2008 ◽  
Vol 49 (1) ◽  
pp. 163 ◽  
Author(s):  
Irina S. Barequet ◽  
Zohar Habot-Wilner ◽  
Nathan Keller ◽  
Gill Smollan ◽  
Hana Ziv ◽  
...  
Keyword(s):  
Amniotic Membrane ◽  
Amniotic Membrane Transplantation ◽  
Bacterial Keratitis

Amniotic Membrane Transplantation in Severe Bacterial Keratitis

Cornea ◽  
2007 ◽  
Vol 26 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Jean-Jacques Gicquel ◽  
Riad A Bejjani ◽  
Pierre Ellies ◽  
Martial Mercié ◽  
Paul Dighiero
Keyword(s):  
Amniotic Membrane ◽  
Amniotic Membrane Transplantation ◽  
Bacterial Keratitis

A randomized clinical trial to evaluate the usefulness of amniotic membrane transplantation in bacterial keratitis healing

2017 ◽  
Vol 15 (2) ◽  
pp. 218-226 ◽  
Author(s):  
Seyed Ali Tabatabaei ◽  
Mohammad Soleimani ◽  
Mahmoud Jabbarvand Behrouz ◽  
Ali Torkashvand ◽  
Pasha Anvari ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Randomized Clinical Trial ◽  
Amniotic Membrane ◽  
Amniotic Membrane Transplantation ◽  
Bacterial Keratitis

scholarly journals The effectiveness of the use of various techniques of amniotic membrane (AM) transplantation in the corneal degenerative processes

2020 ◽  
Vol 8 (3) ◽  
pp. 219-225
Author(s):  
O. V. Shupyk ◽  
A. Y. Mazurkevich ◽  
R. R. Bokotko ◽  
T. V. Dudus
Keyword(s):  
Amniotic Membrane ◽  
Amniotic Membrane Transplantation ◽  
Layer By Layer ◽  
Bacterial Keratitis ◽  
Inflammatory Infiltration ◽  
Experimental Animals ◽  
Long Wavelength ◽  
Transplantation Technique ◽  
Epithelial Cell Layer ◽  
Covering Technique

The amniotic membrane – the innermost of the three membranes, it develops from the fetal ectoderm, it is transparent, avascular and consists of an epithelial cell layer located on the basement membrane and connective tissue stroma. The application efficiency of the amniotic membrane transplantation technique in rabbits with experimental bacterial keratitis has been investigated. The animals were simulated with moderate bacterial keratitis (with preliminary exposure to long-wavelength mercury lamp beams) by the administration to each eye of the Staphylococcus aureus pathogenic strain. On the 14th day, the amniotic membrane was transplanted using two methods: by biological covering with episcleral fixation using simple interrupted sutures and layer-by-layer transplantation with fixation of the membrane within the damage of the cornea with simple interrupted sutures. The degree of the inflammatory process was assessed according to the author’s point scale, which included eight signs. On the 7th, 14th, and 30th days, the experimental animals were euthanized and microscopic examination of the cornea was performed. It was found that on the 7th day of application, complete epithelization of the corneal surface occurred, and on the 30th day, differentiation of its cells into layers. When using the layer-by-layer technique of amniotic membrane transplantation with using simple interrupted sutures, a more pronounced inflammatory reaction was observed in comparison with the biological covering technique. During all observation periods, most experimental animals did not show clinical and morphological signs of inflammatory infiltration. The obtained effect of both methods of amniotic membrane transplantation indicates the effectiveness of using this biological material as the main or supportive in the treatment of severe eye pathologies

scholarly journals Sutureless Amniotic Membrane Transplantation for Severe Bacterial Keratitis

Cornea ◽  
2009 ◽  
Vol 28 (10) ◽  
pp. 1118-1123 ◽  
Author(s):  
Hosam Sheha ◽  
Lingyi Liang ◽  
Jianjing Li ◽  
Scheffer C G Tseng
Keyword(s):  
Amniotic Membrane ◽  
Amniotic Membrane Transplantation ◽  
Bacterial Keratitis

Effect of viable cryopreserved human amniotic membrane sutured to cornea on course of experimental bacterial keratitis

2015 ◽  
Vol 57 (5) ◽  
pp. 35-41
Author(s):  
K. Sereda ◽  
◽  
G. Drozhzhina ◽  
T. Gaidamaka ◽  
V. Vit ◽  
...  
Keyword(s):  
Amniotic Membrane ◽  
Human Amniotic Membrane ◽  
Bacterial Keratitis

scholarly journals Two case reports of continued progression of chronic ocular graft-versus-host disease without concurrent systemic comorbidities treated by amniotic membrane transplantation

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Hideto Ikarashi ◽  
Naohiko Aketa ◽  
Eisuke Shimizu ◽  
Yoji Takano ◽  
Tetsuya Kawakita ◽  
...  
Keyword(s):  
Graft Versus Host Disease ◽  
Amniotic Membrane ◽  
Case Reports ◽  
Signs And Symptoms ◽  
Amniotic Membrane Transplantation ◽  
Team Approach ◽  
Host Disease ◽  
Graft Versus Host ◽  
Target Organs ◽  
Conjunctival Inflammation

Abstract Background Chronic ocular graft-versus-host disease (oGVHD) is an ocular comorbidity of graft-versus-host disease (GVHD) that usually occurs concurrently with systemic manifestations. Failure to detect and treat oGVHD in its early stages may lead to progression of ocular signs and symptoms leading to oGVHD that is refractory to conventional treatment. Case presentation We report the clinical course of a 19-year-old male and a 59-year-old female with severe and progressive chronic oGVHD without concurrent systemic signs of chronic graft-versus-host disease (cGVHD). Although their systemic conditions had been stable, both suffered from severe oGVHD and were referred to our clinic. Both cases exhibited marked improvement in conjunctival inflammation and fibrotic changes after amniotic membrane transplantation (AMT). Both cases underwent keratoplasty eventually to stabilize ocular surface conditions and to improve visual function. Conclusions We reported the clinical outcomes of 2 cases of chronic oGVHD without concurrent systemic comorbidities that were treated with AMT. The clinician should be aware that cGVHD may persist in target organs even in the absence of concurrent systemic comorbidities following seemingly successful systemic treatment. A multidisciplinary team approach is essential in the early detection and therapeutic intervention for chronic oGVHD.

scholarly journals Comparison of amniotic membrane transplantation with and without cultured limbal epithelium for persistent corneal ulcers

2021 ◽  
Vol 35 (1) ◽  
pp. 739-745
Author(s):  
Rozaliya Hristova ◽  
Yani Zdravkov ◽  
Georgi Markov ◽  
Davide Borroni ◽  
Alexander Oscar ◽  
...  
Keyword(s):  
Amniotic Membrane ◽  
Amniotic Membrane Transplantation ◽  
Corneal Ulcers ◽  
Limbal Epithelium

scholarly journals Nano-Scale Modifications of Amniotic Membrane Induced by UV and Antibiotic Treatment: Histological, AFM and FTIR Spectroscopy Evidence

Materials ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 863
Author(s):  
Simona Cavalu ◽  
George Roiu ◽  
Ovidiu Pop ◽  
Denisa A. Petricas Heredea ◽  
Traian Octavian Costea ◽  
...  
Keyword(s):  
Ftir Spectroscopy ◽  
Antibiotic Treatment ◽  
Amniotic Membrane ◽  
Ocular Surface ◽  
Enzymatic Digestion ◽  
Uv Exposure ◽  
Amniotic Membrane Transplantation ◽  
Corneal Tissue ◽  
Nano Scale ◽  
Gentamicin Treatment

The efficiency of amniotic membrane (AM) transplantation in different types of ocular surface disorders is due to its outstanding properties such as antifibrotic, antibacterial, anti-inflammatory and antiangiogenic, working as a versatile scaffold to promote corneal tissue epithelialization. A proper preparation, preservation and clinical application are crucial for the best outcomes in the treatment of different severe ocular disorders, taking into account its fragility. In this context, by combining high-sensitivity tools such as atomic force microscopy (AFM) and Fourier transform infrared (FTIR) spectroscopy with histological and immunohistochemical examination, we aimed to investigate the ultrastructural modifications of the amniotic membrane (AM) upon UV exposure and/or antibiotic treatment, with relevance for clinical applications in ocular surface surgery. From the morphological point of view, we noticed a loss of cuboidal cells in the basal membrane, accompanied by the splitting of collagen fibers upon UV and/or gentamicin treatment, while structural alteration of proteins was evidenced by the FTIR quantitative analysis of the secondary structure. A decrease in α-helix and β-sheet content, accompanied by increased content in less ordered structures (turns, random and side chains), was noticed after all the treatments. At the nano-scale, AFM details showed modifications of collagen fibrils in terms of their thickness and network compaction upon gentamicin and/or UV treatment. The enzymatic digestion assay demonstrated that UV exposure significantly reduces the degradation rate of the AM, while gentamicin treatment promotes an accelerated enzymatic digestion upon UV exposure. In order to highlight the clinical impact of the research, a clinical case is presented showing the relevance of amniotic membrane transplantation in pterygium surgery.

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