Experimental Induction and Three-Dimensional Two-Photon Imaging of Conjunctiva-Associated Lymphoid Tissue

2008 ◽  
Vol 49 (4) ◽  
pp. 1512 ◽  
Author(s):  
Philipp Steven ◽  
Jan Rupp ◽  
Gereon Hu¨ttmann ◽  
Norbert Koop ◽  
Carmen Lensing ◽  
...  
Keyword(s):  
Lymphoid Tissue ◽  
Three Dimensional ◽  
Two Photon ◽  
Experimental Induction ◽  
Photon Imaging ◽  
Two Photon Imaging

scholarly journals Three dimensional two-photon imaging of neuronal activity in freely moving mice using a miniature fiber coupled microscope with active axial-scanning

2018 ◽  
Author(s):  
Baris N. Ozbay ◽  
Gregory L. Futia ◽  
Ming Ma ◽  
Victor M. Bright ◽  
Juliet T. Gopinath ◽  
...  
Keyword(s):  
Dynamic Control ◽  
Three Dimensional ◽  
Neuron Activity ◽  
Two Photon ◽  
Neuronal Imaging ◽  
Freely Moving ◽  
Photon Imaging ◽  
Two Photon Imaging ◽  
Working Distance ◽  
Axial Scanning

AbstractWe present a miniature head mounted two-photon fiber-coupled microscope (2P-FCM) for neuronal imaging with active axial focusing enabled using a miniature electrowetting lens. Full three-dimensional two-photon imaging of GCaMP6s showing individual neuron activity in multiple focal planes was achieved in a freely-moving mouse. Two-color simultaneous imaging of GFP and tdTomato fluorescence is also demonstrated. Additionally, dynamic control of the axial scanning of the electrowetting lens allows tilting of the focal plane enabling cells in multiple focal planes to be imaged simultaneously. Two-photon imaging allows increased penetration depth in tissue yielding a working distance of 450 μm with an additional 180 μm of active axial focusing. The objective NA is 0.45 with a lateral resolution of 1.8 μm, an axial resolution of 10 μm, and a field-of-view of 240 μm diameter. The 2P-FCM has a weight of only ∼2.5 g and is capable of repeatable and stable head-attachment. The 2P-FCM with dynamic axial scanning provides a new capability to record from functionally distinct neuronal layers, opening new opportunities in neuroscience research.

Three-dimensional two-photon imaging in polymeric materials

2002 ◽  
Author(s):  
Kevin D. Belfield ◽  
Katherine J. Schafer ◽  
Stephen Andrasik ◽  
Ozlem Yavuz ◽  
Eric W. Van Stryland ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Polymeric Materials ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging

Biocompatible well-defined chromophore–polymer conjugates for photodynamic therapy and two-photon imaging

2013 ◽  
Vol 4 (1) ◽  
pp. 61-67 ◽  
Author(s):  
Cristina Cepraga ◽  
Thibault Gallavardin ◽  
Sophie Marotte ◽  
Pierre-Henri Lanoë ◽  
Jean-Christophe Mulatier ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Two Photon ◽  
Polymer Conjugates ◽  
Photon Imaging ◽  
Two Photon Imaging
Sign in / Sign up

Export Citation Format

Share Document