Role of Heat Shock Protein 47, a Collagen-Binding Chaperone, in Lacrimal Gland Pathology in Patients with cGVHD

2007 ◽  
Vol 48 (3) ◽  
pp. 1079 ◽  
Author(s):  
Yoko Ogawa ◽  
Mohammed S. Razzaque ◽  
Kaori Kameyama ◽  
Go Hasegawa ◽  
Shigeto Shimmura ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Lacrimal Gland ◽  
Collagen Binding ◽  
Heat Shock Protein 47

Fibrogenic role of Collagen-Binding Heat Shock Protein 47 in Lacrimal Glands of Patients with Chronic Graft versus Host Disease

2005 ◽  
Vol 3 ◽  
pp. S98
Author(s):  
Yoko Ogawa ◽  
Mohammed S. Razzaque ◽  
Masataka Kawai ◽  
Kaori Kameyama ◽  
Yukihiko Mashima ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Graft Versus Host Disease ◽  
Lacrimal Glands ◽  
Collagen Binding ◽  
Host Disease ◽  
Heat Shock Protein 47 ◽  
Graft Versus Host

Role of heat shock protein 47 on tubulointerstitium in experimental radiation nephropathy

2002 ◽  
Vol 52 (5-6) ◽  
pp. 340-347 ◽  
Author(s):  
Diange Liu ◽  
Mohammed S. Razzaque ◽  
Arifa Nazneen ◽  
Takashi Naito ◽  
Takashi Taguchi
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 47 ◽  
Radiation Nephropathy

Abstract 141: Influence of the Collagen Processing Heat Shock Protein 47 on Cardiomyocyte Homeostasis and Maturation

2014 ◽  
Vol 115 (suppl_1) ◽  
Author(s):  
Sumon Sur ◽  
Peter Christalla ◽  
Angelica Roa ◽  
Wolfram H Zimmermann
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Cardiac Tissue ◽  
Isometric Force ◽  
Embryonic Stem ◽  
3D Culture ◽  
Heat Shock Protein 47 ◽  
Culture Models ◽  
2D And 3D

PURPOSE: Heat shock protein 47 (Hsp47) is a collagen-specific molecular chaperone required for maturation of collagen type 1. Little is known about the role of Hsp47 in the heart. This study aims to investigate whether Hsp47 is important for cardiomyocyte maturation and assembly into functional syncytia. METHODS: We made use of recently developed 2D and 3D culture platforms to scrutinize the role of Hsp47 for cardiomyocyte maturation and function, i.e., (1) 2D model: culture of embryonic stem cell-derived cardiomyocytes (ESC-CM) on ECMs derived from both mouse embryonic wild type (WT)- and Hsp47 (-/-) fibroblasts and (2) 3D model: construction of engineered heart muscle (EHM) consisting of purified ESC-CMs with WT- or Hsp47 (-/-) fibroblasts. Cultures were investigated using confocal microscopy and isometric force measurements. RESULTS: ESC-CMs showed mainly immature polygonal shaped morphology and disorganized sarcomeric structures when cultured on ECM secreted by Hsp47 (-/-) fibroblasts; this morphology was in clear contrast to the mostly anisotropic cardiomyocyte appearance in control conditions. EHM with Hsp47 (-/-) fibroblasts did not develop measurable contractile forces. In contrast, WT fibroblast-supplemented EHMs contracted regularly (WT: 0.14±0.1 mN at 2.4 mM Ca2+; n=8/group). Histological analysis of Hsp47 (-/-) EHMs showed mainly rounded and underdeveloped cardiomyocytes comparable to the cardiomyocyte phenotype observed in EHMs containing only ESC-CMs, without fibroblasts. CONCLUSIONS: Hsp47 influences the deposition of ECM-collagen and affects cardiomyocyte morphology and functionality in our 2D and 3D culture models. Our data collectively suggest that Hsp47 may be an attractive target for the regulation of cardiac tissue homeostasis.

205 Role of Heat Shock Protein 47 in the Pathogenesis of Patients With Crohn's Disease

2010 ◽  
Vol 138 (5) ◽  
pp. S-38
Author(s):  
Yusuke Honzawa ◽  
Hiroshi Nakase ◽  
Kayoko Matsumura ◽  
Shuji Yamamoto ◽  
Takuya Yoshino ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Heat Shock ◽  
Crohn's Disease ◽  
Heat Shock Protein ◽  
Heat Shock Protein 47

scholarly journals Involvement of collagen-binding heat shock protein 47 in scleroderma-associated fibrosis

2015 ◽  
Vol 6 (8) ◽  
pp. 589-598 ◽  
Author(s):  
Haiyan Chu ◽  
Ting Wu ◽  
Wenyu Wu ◽  
Wenzhen Tu ◽  
Shuai Jiang ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Collagen Binding ◽  
Heat Shock Protein 47

scholarly journals Role of Heat Shock Protein 47 in Transdifferentiation of Human Tenon's Fibroblasts to Myofibroblasts

2012 ◽  
Vol 12 (1) ◽  
Author(s):  
Samin Hong ◽  
Kyoungsoo Park ◽  
Jin Hyoung Kim ◽  
Sueng-Han Han ◽  
Jong Bok Lee ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 47
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