Altered Gene Expression of Angiogenic Factors Induced by Calcium-Mediated Dissociation of Retinal Pigment Epithelial Cells

2005 ◽  
Vol 46 (4) ◽  
pp. 1508 ◽  
Author(s):  
Xue-Feng Wang ◽  
Jing Z. Cui ◽  
Shiv S. Prasad ◽  
Joanne A. Matsubara
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Retinal Pigment Epithelial ◽  
Retinal Pigment ◽  
Angiogenic Factors ◽  
Retinal Pigment Epithelial Cells ◽  
Altered Gene Expression ◽  
Pigment Epithelial ◽  
Altered Gene ◽  
Pigment Epithelial Cells

scholarly journals The influence of elastin degradation products, glucose and atorvastatin on metalloproteinase-1, -2, -9 and tissue inhibitor of metalloproteinases-1, -2, -3 expression in human retinal pigment epithelial cells.

2014 ◽  
Vol 61 (2) ◽  
Author(s):  
Mariola Dorecka ◽  
Tomasz Francuz ◽  
Wojciech Garczorz ◽  
Krzysztof Siemianowicz ◽  
Wanda Romaniuk
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Retinal Pigment Epithelial ◽  
Degradation Products ◽  
Retinal Pigment ◽  
Retinal Pigment Epithelial Cells ◽  
Dependent Manner ◽  
Pigment Epithelial ◽  
Elastin Degradation ◽  
Pigment Epithelial Cells

Hyperglycemia and increased concentrations of elastin degradation products (EDPs) are common findings in patients with diabetes, atherosclerosis and hypertension. The aim of this study was to assess the influence of high glucose, EDPs and atorvastatin on MMP-1, MMP-2, MMP-9 and TIMP1-3 gene expression in human retinal pigment epithelial cells (HRPE) in vitro. HRPE were cultured for 24 hours with the substances being tested (glucose, EDPs), alone or in combination. Additionally, the cells were treated with atorvastatin in two different concentrations (1 or 10 μM). After incubation, total cellular RNA was extracted and used for gene expression evaluation. Gene expression was measured using the real-time RT-PCR technique. Glucose, EDPs and atorvastatin had no impact on TIMP-1 and TIMP-3 expression. HRPE cells treated with glucose or EDPs with the addition of atorvastatin had a statistically significant decrease of TIMP-2 expression; glucose alone decreased MMP-1 expression. Atorvastatin decreased expression of all assessed genes, except TIMP-1 and TIMP-3 in a dose-dependent manner. Our results confirm the importance of MMPs and TIMPs in retinal vascular biology. Atorvastatin-induced MMPs gene expression can deeply affect extracellular matrix turnover, which may play an important role in the progression of ocular diseases.

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