scholarly journals Contrast Sensitivity, Spatial and Temporal Tuning of the Larval Zebrafish Optokinetic Response

2005 ◽  
Vol 46 (1) ◽  
pp. 137 ◽  
Author(s):  
Oliver Rinner ◽  
Jens M. Rick ◽  
Stephan C. F. Neuhauss
Keyword(s):  
Contrast Sensitivity ◽  
Optokinetic Response ◽  
Larval Zebrafish

Directional Asymmetries in the Optokinetic Response of Larval Zebrafish (Danio rerio)

Zebrafish ◽  
2005 ◽  
Vol 2 (3) ◽  
pp. 189-196 ◽  
Author(s):  
Haohua Qian ◽  
Ying Zhu ◽  
David J. Ramsey ◽  
Richard L. Chappell ◽  
John E. Dowling ◽  
...  
Keyword(s):  
Danio Rerio ◽  
Optokinetic Response ◽  
Larval Zebrafish ◽  
Directional Asymmetries

scholarly journals Spherical arena reveals optokinetic response tuning to stimulus location, size, and frequency across entire visual field of larval zebrafish

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Florian Alexander Dehmelt ◽  
Rebecca Meier ◽  
Julian Hinz ◽  
Takeshi Yoshimatsu ◽  
Clara A Simacek ◽  
...  
Keyword(s):  
Visual Field ◽  
Frequency Tuning ◽  
Sample Stimulus ◽  
Visual Fields ◽  
Visual Space ◽  
Motion Vision ◽  
Optokinetic Response ◽  
Larval Zebrafish ◽  
Retinal Processing ◽  
Entire Visual Field

Many animals have large visual fields, and sensory circuits may sample those regions of visual space most relevant to behaviours such as gaze stabilisation and hunting. Despite this, relatively small displays are often used in vision neuroscience. To sample stimulus locations across most of the visual field, we built a spherical stimulus arena with 14,848 independently controllable LEDs. We measured the optokinetic response gain of immobilised zebrafish larvae to stimuli of different steradian size and visual field locations. We find that the two eyes are less yoked than previously thought and that spatial frequency tuning is similar across visual field positions. However, zebrafish react most strongly to lateral, nearly equatorial stimuli, consistent with previously reported spatial densities of red, green and blue photoreceptors. Upside-down experiments suggest further extra-retinal processing. Our results demonstrate that motion vision circuits in zebrafish are anisotropic, and preferentially monitor areas with putative behavioural relevance.

scholarly journals Affordable and effective optokinetic response methods to assess visual acuity and contrast sensitivity in larval to juvenile zebrafish

2022 ◽  
Vol 1 ◽  
pp. 92
Author(s):  
Alicia Gómez Sánchez ◽  
Yolanda Álvarez ◽  
Basilio Colligris ◽  
Breandán N. Kennedy
Keyword(s):  
Visual Acuity ◽  
Contrast Sensitivity ◽  
Cost Effective ◽  
Early Morning ◽  
Time Of Day ◽  
Data Consistency ◽  
Optokinetic Response ◽  
Early Afternoon ◽  
3D Printed ◽  
Functional Vision

The optokinetic response (OKR) is an effective behavioural assay to investigate functional vision in zebrafish. The rapid and widespread use of gene editing, drug screening and environmental modulation technologies has resulted in a broader need for visual neuroscience researchers to access affordable and more sensitive OKR, contrast sensitivity (CS) and visual acuity (VA) assays. Here, we demonstrate how 2D- and 3D-printed, striped patterns or drums coupled with a motorised base and microscope provide a simple, cost-effective but efficient means to assay OKR, CS and VA in larval-juvenile zebrafish. In wild-type, five days post-fertilisation (dpf) zebrafish, the 2D or 3D set-ups of 0.02 cycles per degree (cpd) (standard OKR stimulus) and 100% black-white contrast evoked equivalent responses of 24.2±3.9 or 21.8±3.9 saccades per minute, respectively. Furthermore, although the OKR number was significantly reduced compared to the 0.02 cpd drum (p<0.0001), 0.06 and 0.2 cpd drums elicited equivalent responses with both set-ups. Notably, standard OKRs varied with time of day; peak responses of 29.8±7 saccades per minute occurred in the early afternoon with significantly reduced responses occurring in the early morning or late afternoon (18.5±3 and 18.4±4.5 saccades per minute, respectively). A customised series of 2D printed drums enabled analysis of VA and CS in 5-21 dpf zebrafish. The saccadic frequency in VA assays was inversely proportional to age and spatial frequency and in CS assays was inversely proportional to age and directly proportional to contrast of the stimulus. OKR, VA and CS of zebrafish larvae can be efficiently measured using 2D- or 3D-printed striped drums. For data consistency the luminance of the OKR light source, the time of day when the analysis is performed, and the order of presentation of VA and CS drums must be considered. These simple methods allow effective and more sensitive analysis of functional vision in zebrafish.

scholarly journals Affordable and Effective Optokinetic Response Methods to Assess Visual Acuity and Contrast Sensitivity in Larval to Juvenile Zebrafish

2021 ◽  
Author(s):  
Alicia Gómez Sánchez ◽  
Yolanda Álvarez ◽  
Basilio Colligris ◽  
Breandán N. Kennedy
Keyword(s):  
Visual Acuity ◽  
Contrast Sensitivity ◽  
Early Morning ◽  
Time Of Day ◽  
Data Consistency ◽  
Optokinetic Response ◽  
Early Afternoon ◽  
3D Printed ◽  
Functional Vision ◽  
2D And 3D

AbstractBackgroundThe optokinetic response (OKR) is an effective behavioural assay to investigate functional vision in zebrafish. The rapid and widespread use of gene editing, drug screening and environmental modulation technologies have resulted in a broader need for visual neuroscience researchers to access affordable and more sensitive OKR, contrast sensitivity (CS) and visual acuity (VA) assays. Here, we demonstrate how 2D- and 3D-printed, striped patterns or drums coupled with a motorised base and microscope provide a simple, cost-effective but efficient means to assay OKR, CS and VA in larval-juvenile zebrafish.ResultsIn wild-type, 5 days post-fertilisation (dpf) zebrafish, the 2D or 3D drums printed with the standard OKR stimulus of 0.02 cycles per degree (cpd), 100% black-white contrast evoked equivalent responses of 24.2 or 21.8 saccades per minute, respectively. Furthermore, although the OKR number was significantly reduced compared to the 0.02 cpd drum (p<0.0001), the 2D and 3D drums evoked respectively equivalent responses with the 0.06 and 0.2 cpd drums. Notably, standard OKR responses varied with time of day; peak responses of 29.8 saccades per minute occurred in the early afternoon with significantly reduced responses occurring in the early morning or late afternoon, (18.5 and 18.4 saccades per minute, respectively). A customised series of 2D printed drums enabled analysis of visual acuity and contrast sensitivity in 5-21 dpf zebrafish. The saccadic frequency in visual acuity and contrast sensitivity assays, was inversely proportional to age, spatial frequency and contrast of the stimulus.ConclusionsOKR, VA and CS of zebrafish larvae can be efficiently measured using 2D- or 3D-printed striped drums. For data consistency the luminance of the OKR light source, the time of day when the analysis performed, and the order of presentation of VA and CS drums must be considered. These simple methods allow effective and more sensitive analysis of functional vision in zebrafish.

scholarly journals Circuit Organization Underlying Optic Flow Processing in Zebrafish

2021 ◽  
Vol 15 ◽  
Author(s):  
Koji Matsuda ◽  
Fumi Kubo
Keyword(s):  
Optic Flow ◽  
Visual Features ◽  
Optomotor Response ◽  
Imaging Studies ◽  
Optokinetic Response ◽  
Larval Zebrafish ◽  
Specific Manner ◽  
Flow Information ◽  
Optic Flow Information ◽  
Self Motion

Animals’ self-motion generates a drifting movement of the visual scene in the entire field of view called optic flow. Animals use the sensation of optic flow to estimate their own movements and accordingly adjust their body posture and position and stabilize the direction of gaze. In zebrafish and other vertebrates, optic flow typically drives the optokinetic response (OKR) and optomotor response (OMR). Recent functional imaging studies in larval zebrafish have identified the pretectum as a primary center for optic flow processing. In contrast to the view that the pretectum acts as a relay station of direction-selective retinal inputs, pretectal neurons respond to much more complex visual features relevant to behavior, such as spatially and temporally integrated optic flow information. Furthermore, optic flow signals, as well as motor signals, are represented in the cerebellum in a region-specific manner. Here we review recent findings on the circuit organization that underlies the optic flow processing driving OKR and OMR.

scholarly journals Affordable and effective optokinetic response methods to assess visual acuity and contrast sensitivity in larval to juvenile zebrafish

2021 ◽  
Vol 1 ◽  
pp. 92
Author(s):  
Alicia Gómez Sánchez ◽  
Yolanda Álvarez ◽  
Basilio Colligris ◽  
Breandán N. Kennedy
Keyword(s):  
Visual Acuity ◽  
Contrast Sensitivity ◽  
Early Morning ◽  
Time Of Day ◽  
Data Consistency ◽  
Optokinetic Response ◽  
Early Afternoon ◽  
3D Printed ◽  
Functional Vision ◽  
2D And 3D

The optokinetic response (OKR) is an effective behavioural assay to investigate functional vision in zebrafish. The rapid and widespread use of gene editing, drug screening and environmental modulation technologies has resulted in a broader need for visual neuroscience researchers to access affordable and more sensitive OKR, contrast sensitivity (CS) and visual acuity (VA) assays. Here, we demonstrate how 2D- and 3D-printed, striped patterns or drums coupled with a motorised base and microscope provide a simple, cost-effective but efficient means to assay OKR, CS and VA in larval-juvenile zebrafish. In wild-type, five days post-fertilisation (dpf) zebrafish, the 2D or 3D drums printed with the standard OKR stimulus of 0.02 cycles per degree (cpd), 100% black-white contrast evoked equivalent responses of 24.2 or 21.8 saccades per minute, respectively. Furthermore, although the OKR number was significantly reduced compared to the 0.02 cpd drum (p<0.0001), the 2D and 3D drums evoked equivalent responses with the 0.06 and 0.2 cpd drums. Notably, standard OKRs varied with time of day; peak responses of 29.8 saccades per minute occurred in the early afternoon with significantly reduced responses occurring in the early morning or late afternoon (18.5 and 18.4 saccades per minute, respectively). A customised series of 2D printed drums enabled analysis of VA and CS in 5-21 dpf zebrafish. The saccadic frequency in VA and CS assays was inversely proportional to age, spatial frequency and contrast of the stimulus. OKR, VA and CS of zebrafish larvae can be efficiently measured using 2D- or 3D-printed striped drums. For data consistency the luminance of the OKR light source, the time of day when the analysis is performed, and the order of presentation of VA and CS drums must be considered. These simple methods allow effective and more sensitive analysis of functional vision in zebrafish.

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