Survival of Retinal Pigment Epithelium after Exposure to Prolonged Oxidative Injury: A Detailed Gene Expression and Cellular Analysis

2004 ◽  
Vol 45 (10) ◽  
pp. 3767 ◽  
Author(s):  
Nataly Strunnikova ◽  
Connie Zhang ◽  
Diane Teichberg ◽  
Scott W. Cousins ◽  
Judit Baffi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Retinal Pigment Epithelium ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Oxidative Injury ◽  
Cellular Analysis

scholarly journals Comparative gene expression study and pathway analysis of the human iris- and the retinal pigment epithelium

PLoS ONE ◽  
2017 ◽  
Vol 12 (8) ◽  
pp. e0182983 ◽  
Author(s):  
Anna Bennis ◽  
Jacoline B. ten Brink ◽  
Perry D. Moerland ◽  
Vivi M. Heine ◽  
Arthur A. Bergen
Keyword(s):  
Gene Expression ◽  
Retinal Pigment Epithelium ◽  
Pathway Analysis ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Gene Expression Study ◽  
Expression Study ◽  
Human Iris

scholarly journals Human iPSC-derived retinal pigment epithelium: a model system for identifying and functionally characterizing causal variants at AMD risk loci

2018 ◽  
Author(s):  
Erin N. Smith ◽  
Agnieszka D’Antonio-Chronowska ◽  
William W. Greenwald ◽  
Victor Borja ◽  
Lana R. Aguiar ◽  
...  
Keyword(s):  
Gene Expression ◽  
Retinal Pigment Epithelium ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Induced Pluripotent Stem Cell ◽  
Age Related Macular Degeneration ◽  
Molecular Characteristics ◽  
Specific Expression ◽  
Age Related ◽  
Causal Variants

SummaryWe evaluate whether human induced pluripotent stem cell-derived retinal pigment epithelium (iPSC-RPE) cells can be used to prioritize and functionally characterize causal variants at age-related macular degeneration (AMD) risk loci. We generated iPSC-RPE from six subjects and show that they have morphological and molecular characteristics similar to native RPE. We generated RNA-seq, ATAC-seq, and H3K27ac ChIP-seq data and observe high similarity in gene expression and enriched transcription factor motif profiles between iPSC-RPE and human fetal-RPE. We performed fine-mapping of AMD risk loci by integrating molecular data from the iPSC-RPE, adult retina, and adult RPE, which identified rs943080 as the probable causal variant at VEGFA. We show that rs943080 is associated with altered chromatin accessibility of a distal ATAC-seq peak, decreased overall gene expression of VEGFA, and allele specific expression of a non-coding transcript. These results provide insight into the mechanism underlying the association of the VEGFA locus with AMD.

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