Regulatory Role of PI 3-Kinase on Expression of Cdk4 and p27, Nuclear Localization of Cdk4, and Phosphorylation of p27 in Corneal Endothelial Cells

2003 ◽  
Vol 44 (4) ◽  
pp. 1521 ◽  
Author(s):  
Hyung Taek Lee ◽  
EunDuck P. Kay
Keyword(s):  
Endothelial Cells ◽  
Nuclear Localization ◽  
Regulatory Role ◽  
Corneal Endothelial Cells

Role of Glutathione Peroxidase 4 in Corneal Endothelial Cells

2016 ◽  
Vol 42 (3) ◽  
pp. 380-385 ◽  
Author(s):  
Takatoshi Uchida ◽  
Osamu Sakai ◽  
Hirotaka Imai ◽  
Takashi Ueta
Keyword(s):  
Endothelial Cells ◽  
Glutathione Peroxidase ◽  
Corneal Endothelial Cells

Role of Human Corneal Endothelial Cells in T-Cell–Mediated Alloimmune Attack In Vitro

2014 ◽  
Vol 55 (3) ◽  
pp. 1213 ◽  
Author(s):  
Imad Lahdou ◽  
Christoph Engler ◽  
Stefan Mehrle ◽  
Volker Daniel ◽  
Mahmoud Sadeghi ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
T Cell ◽  
Corneal Endothelial Cells ◽  
Human Corneal Endothelial Cells

A Distinct Regulatory Role of Th17 Cytokines IL-17A and IL-17F in Chemokine Secretion from Lung Microvascular Endothelial Cells

Inflammation ◽  
2012 ◽  
Vol 35 (3) ◽  
pp. 1119-1131 ◽  
Author(s):  
Hitomi Fujie ◽  
Kaijun Niu ◽  
Michiru Ohba ◽  
Yoshihisa Tomioka ◽  
Haruki Kitazawa ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Regulatory Role ◽  
Microvascular Endothelial Cells ◽  
Th17 Cytokines ◽  
Microvascular Endothelial

scholarly journals A Novel Mutation in the Human Androgen Receptor Suggests a Regulatory Role for the Hinge Region in Amino-Terminal and Carboxy-Terminal Interactions

2008 ◽  
Vol 93 (10) ◽  
pp. 3691-3696 ◽  
Author(s):  
A. Deeb ◽  
J. Jääskeläinen ◽  
M. Dattani ◽  
H. C. Whitaker ◽  
C. Costigan ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Nuclear Localization ◽  
Novel Mutation ◽  
Poor Response ◽  
Fold Increase ◽  
Hinge Region ◽  
Regulatory Role ◽  
Amino Terminal ◽  
Androgen Binding

Context: The androgen insensitivity syndrome (AIS) is caused by molecular defects in the androgen receptor (AR). Clinically, the partial AIS has a variable phenotype. Many mechanisms explain the phenotype in the AIS. A crucial step in AR action is the interaction of the N and C termini. Objective: The role of the hinge region of the AR is not as well understood as other parts of the receptor. We aim to study the role of this region in the N/C-termini interaction. Patient and Method: We report a patient with severe undermasculinization and poor response to exogenous androgens. Androgen binding was performed, and the AR gene was sequenced. The mutation was recreated and transfected in COS-1 cells. Transactivation was studied. N/C-termini interaction was studied using a mammalian two-hybrid assay. A nuclear localization study was performed. Results: Androgen binding was normal, and a novel mutation (Arg629Trp) in the AR hinge region was identified. Mutant AR transactivation was 40% higher compared with wild type (WT). A 3-fold increase in transcription occurred when both WT N and C-terminal domains were cotransfected; no response occurred when the mutated region of the AR was included (P < 0.001). Cells with mutant AR showed a comparable nuclear localization to the WT AR. Conclusions: A mutation in the hinge region impaired N/C-domain interaction in the presence of normal AR binding and nuclear localization. It resulted in severe undermasculinization at birth and resistance to androgens. The findings confirm a unique regulatory role for the hinge region in AR function.

Sign in / Sign up

Export Citation Format

Share Document