Fluorescent ZnO Quantum Dot Probe to Study Glucose–Glucose Oxidase Interaction via Fluorescence Resonance Energy Transfer

2020 ◽  
Vol 18 (5) ◽  
pp. 351-365
Author(s):  
Awadhesh Kumar Verma ◽  
Z. A. Ansari
Keyword(s):  
Sol Gel ◽  
Synthesis Method ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Cd Spectroscopy ◽  
Morphological Characterization ◽  
Visible Absorption ◽  
Tauc Plot ◽  
Donor And Acceptor ◽  
Donor Acceptor

In this work, emphasis is to utilize FRET method to analyze GOx and glucose interaction using fluorescent ZnO QDs as the probes. Fluorescent ZnO and ZnOext QDs were synthesized using sol– gel technique and green synthesis method as donor and acceptor nanoprobes. Structural, optical and morphological characterization of QDS were carried out using UV-visible absorption, fluorescence, FTIR, XRD and field emission scanning electron microscopy. Band gap estimated from Tauc plot is 3.49 and 3.35 eV for ZnO and ZnOext QDs and XRD reveal Wurtzite structure of grown crystals. Systematic absorption study for ZnO–GOx and ZnOext–GOx reveal association constant of –8.3361 M–1 and –2.57646 M –1 for ZnO and ZnOext using Benesi-Hildebrand plot. The binding constant obtained from Stern-Volmer equation is 1.0466 μM –1 and 1.97 μM –1 for ZnO/ZnOext–GOx conjugate and suggest static quenching in the system. CD spectroscopy reveal native state of protein in conjugate sytem. Constant Förster radius (Ro) in ZnOext–GOx system suggest the average 〈κ2〉 as 2/3 and is independent of donor–acceptor distance as normally assumed in FRET system varies from ∼4.5% in ZnO–GOx and ∼5% in ZnOext–GOx system. It indicate better energy transferrin earlier system than formal. The physiological range of glucose from 60 mg–440 mg/dl using this technique in which ZnOext–GOx as donor probe exhibit better response towards glucose than ZnO–GOx system and correlated to the presence of phyotochemicals on the QD surface.

scholarly journals Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer

2004 ◽  
Vol 381 (1) ◽  
pp. 307-312 ◽  
Author(s):  
Satoshi KARASAWA ◽  
Toshio ARAKI ◽  
Takeharu NAGAI ◽  
Hideaki MIZUNO ◽  
Atsushi MIYAWAKI
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Fluorescent Proteins ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Acceptor Pair ◽  
Donor And Acceptor ◽  
Donor Acceptor ◽  
Donor Acceptor Pair ◽  
Fluorescence Resonance

GFP (green fluorescent protein)-based FRET (fluorescence resonance energy transfer) technology has facilitated the exploration of the spatio-temporal patterns of cellular signalling. While most studies have used cyan- and yellow-emitting FPs (fluorescent proteins) as FRET donors and acceptors respectively, this pair of proteins suffers from problems of pH-sensitivity and bleeding between channels. In the present paper, we demonstrate the use of an alternative additional donor/acceptor pair. We have cloned two genes encoding FPs from stony corals. We isolated a cyan-emitting FP from Acropara sp., whose tentacles exhibit cyan coloration. Similar to GFP from Renilla reniformis, the cyan FP forms a tight dimeric complex. We also discovered an orange-emitting FP from Fungia concinna. As the orange FP exists in a complex oligomeric structure, we converted this protein into a monomeric form through the introduction of three amino acid substitutions, recently reported to be effective for converting DsRed into a monomer (Clontech). We used the cyan FP and monomeric orange FP as a donor/acceptor pair to monitor the activity of caspase 3 during apoptosis. Due to the close spectral overlap of the donor emission and acceptor absorption (a large Förster distance), substantial pH-resistance of the donor fluorescence quantum yield and the acceptor absorbance, as well as good separation of the donor and acceptor signals, the new pair can be used for more effective quantitative FRET imaging.

scholarly journals Anharmonic Molecular Motion Drives Resonance Energy Transfer in peri-Arylene Dyads

2020 ◽  
Vol 8 ◽  
Author(s):  
Vladislav Sláma ◽  
Václav Perlík ◽  
Heinz Langhals ◽  
Andreas Walter ◽  
Tomáš Mančal ◽  
...  
Keyword(s):  
Quantum Chemical ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Unified Framework ◽  
Time Resolved ◽  
Transport Dynamics ◽  
Donor And Acceptor ◽  
Donor Acceptor ◽  
Non Gaussian ◽  
Good Agreement

Spectral and dynamical properties of molecular donor-acceptor systems strongly depend on the steric arrangement of the constituents with exciton coupling J as a key control parameter. In the present work we study two peri-arylene based dyads with orthogonal and parallel transition dipoles for donor and acceptor moieties, respectively. We show that the anharmonic multi-well character of the orthogonal dyad's intramolecular potential explains findings from both stationary and time-resolved absorption experiments. While for a parallel dyad, standard quantum chemical estimates of J at 0 K are in good agreement with experimental observations, J becomes vanishingly small for the orthogonal dyad, in contrast to its ultrafast experimental transfer times. This discrepancy is not resolved even by accounting for harmonic fluctuations along normal coordinates. We resolve this problem by supplementing quantum chemical approaches with dynamical sampling of fluctuating geometries. In contrast to the moderate Gaussian fluctuations of J for the parallel dyad, fluctuations for the orthogonal dyad are found to follow non-Gaussian statistics leading to significantly higher effective J in good agreement with experimental observations. In effort to apply a unified framework for treating the dynamics of optical coherence and excitonic populations of both dyads, we employ a vibronic approach treating electronic and selected vibrational degrees on an equal footing. This vibronic model is used to model absorption and fluorescence spectra as well as donor-acceptor transport dynamics and covers the more traditional categories of Förster and Redfield transport as limiting cases.

Quantum Dot Bioconjugates as Energy Donors in Fluorescence Resonance Energy Transfer Assays

2003 ◽  
Vol 773 ◽  
Author(s):  
Aaron R. Clapp ◽  
Igor L. Medintz ◽  
J. Matthew Mauro ◽  
Hedi Mattoussi
Keyword(s):  
Energy Transfer ◽  
Quantum Dot ◽  
Organic Dyes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Genetically Engineered ◽  
Molar Ratio ◽  
Binding Assays ◽  
Specific Sequence ◽  
Donor Acceptor

AbstractLuminescent CdSe-ZnS core-shell quantum dot (QD) bioconjugates were used as energy donors in fluorescent resonance energy transfer (FRET) binding assays. The QDs were coated with saturating amounts of genetically engineered maltose binding protein (MBP) using a noncovalent immobilization process, and Cy3 organic dyes covalently attached at a specific sequence to MBP were used as energy acceptor molecules. Energy transfer efficiency was measured as a function of the MBP-Cy3/QD molar ratio for two different donor fluorescence emissions (different QD core sizes). Apparent donor-acceptor distances were determined from these FRET studies, and the measured distances are consistent with QD-protein conjugate dimensions previously determined from structural studies.

scholarly journals Distribution of a Glycosylphosphatidylinositol-anchored Protein at the Apical Surface of MDCK Cells Examined at a Resolution of <100 Å Using Imaging Fluorescence Resonance Energy Transfer

1998 ◽  
Vol 142 (1) ◽  
pp. 69-84 ◽  
Author(s):  
A.K. Kenworthy ◽  
M. Edidin
Keyword(s):  
Energy Transfer ◽  
Lipid Rafts ◽  
Fluorescence Resonance Energy Transfer ◽  
Mdck Cells ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Membrane Microdomains ◽  
Apical Surface ◽  
Donor And Acceptor ◽  
Fluorescence Resonance

Membrane microdomains (“lipid rafts”) enriched in glycosylphosphatidylinositol (GPI)-anchored proteins, glycosphingolipids, and cholesterol have been implicated in events ranging from membrane trafficking to signal transduction. Although there is biochemical evidence for such membrane microdomains, they have not been visualized by light or electron microscopy. To probe for microdomains enriched in GPI- anchored proteins in intact cell membranes, we used a novel form of digital microscopy, imaging fluorescence resonance energy transfer (FRET), which extends the resolution of fluorescence microscopy to the molecular level (&lt;100 Å). We detected significant energy transfer between donor- and acceptor-labeled antibodies against the GPI-anchored protein 5′ nucleotidase (5′ NT) at the apical membrane of MDCK cells. The efficiency of energy transfer correlated strongly with the surface density of the acceptor-labeled antibody. The FRET data conformed to theoretical predictions for two-dimensional FRET between randomly distributed molecules and were inconsistent with a model in which 5′ NT is constitutively clustered. Though we cannot completely exclude the possibility that some 5′ NT is in clusters, the data imply that most 5′ NT molecules are randomly distributed across the apical surface of MDCK cells. These findings constrain current models for lipid rafts and the membrane organization of GPI-anchored proteins.

Controlling fluorescence resonance energy transfer of donor–acceptor dyes by Diels–Alder dynamic covalent bonds

2021 ◽  
Vol 57 (26) ◽  
pp. 3275-3278
Author(s):  
Yanhui Cui ◽  
Fen Li ◽  
Xin Zhang
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Aromatic Amine ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Diels Alder ◽  
Covalent Bonds ◽  
Donor Acceptor ◽  
Fluorescence Resonance ◽  
Mutual Conversion

Two new dyes, consisting of an aromatic amine donor and dansyl acceptor connected by Diels–Alder bonds, display a switchable energy transfer. Dynamic covalent properties enable the mutual conversion of the two dyes by maleimide exchanges.

scholarly journals Ureasil organic–inorganic hybrids as photoactive waveguides for conjugated polyelectrolyte luminescent solar concentrators

2017 ◽  
Vol 1 (11) ◽  
pp. 2271-2282 ◽  
Author(s):  
Ilaria Meazzini ◽  
Camille Blayo ◽  
Jochen Arlt ◽  
Ana-Teresa Marques ◽  
Ullrich Scherf ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Solar Concentrators ◽  
Conjugated Polyelectrolyte ◽  
Luminescent Solar Concentrators ◽  
Donor Acceptor

We test the potential of resonance energy transfer to enhance the performance of conjugated copolyelectrolyte donor–acceptor luminescent solar concentrators immobilised within a photoactive organic–inorganic ureasil waveguide.

scholarly journals Development of FRET-based indicators for visualizing homophilic trans interaction of a clustered protocadherin

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Takashi Kanadome ◽  
Natsumi Hoshino ◽  
Takeharu Nagai ◽  
Tomoki Matsuda ◽  
Takeshi Yagi
Keyword(s):  
Fluorescent Proteins ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Direct Visualization ◽  
Binding Properties ◽  
New Approach ◽  
Self Recognition ◽  
Highly Sensitive ◽  
Donor And Acceptor ◽  
Clustered Protocadherins

AbstractClustered protocadherins (Pcdhs), which are cell adhesion molecules, play a fundamental role in self-recognition and non-self-discrimination by conferring diversity on the cell surface. Although systematic cell-based aggregation assays provide information regarding the binding properties of Pcdhs, direct visualization of Pcdh trans interactions across cells remains challenging. Here, we present Förster resonance energy transfer (FRET)-based indicators for directly visualizing Pcdh trans interactions. We developed the indicators by individually inserting FRET donor and acceptor fluorescent proteins (FPs) into the ectodomain of Pcdh molecules. They enabled successful visualization of specific trans interactions of Pcdh and revealed that the Pcdh trans interaction is highly sensitive to changes in extracellular Ca2+ levels. We expect that FRET-based indicators for visualizing Pcdh trans interactions will provide a new approach for investigating the roles of Pcdh in self-recognition and non-self-discrimination processes.

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