A New Method for Decolorization Study of Trypan Blue and Methylene Blue by Bacterial Laccase: Nonlinear Optic

2019 ◽  
Vol 17 (4) ◽  
pp. 312-317
Author(s):  
Afshin Azarpour ◽  
Elaheh Asadi Shamsabadi ◽  
Ali Makhdoumi ◽  
Soheil Sharifi
Keyword(s):  
Methylene Blue ◽  
Nonlinear Optic ◽  
Trypan Blue ◽  
New Method ◽  
Bacterial Laccase

scholarly journals A mammary gland whole mount technique that preserves cell fine structure for electron miscroscopy.

1979 ◽  
Vol 27 (9) ◽  
pp. 1271-1274 ◽  
Author(s):  
J M Strum
Keyword(s):  
Electron Microscopy ◽  
Methylene Blue ◽  
Fine Structure ◽  
Mammary Gland ◽  
Trypan Blue ◽  
Negative Image ◽  
Whole Mount ◽  
Microscope Slides ◽  
Glass Microscope ◽  
Unusual Finding

A mammary gland whole mount technique has been developed that preserves cell fine structure and makes it possible to also examine the preparations by electron microscopy. The glands are placed on glass microscope slides, fixed in a paraformaldehyde-glutaraldehyde mixture, defatted in acetone, stained with 0.5% methylene blue (or trypan blue) in saline, and dehydrated in ethanol. They are evaluated and photographed in 100% ethanol. Then specific areas (i.e. containing small growths, tumors, or other lesions) are selected, excised and prepared for electroscopy. The ultrastructural preservation is good, organelles are evident and there is no observable dye precipitate. The only unusual finding is that cell membranes display a "negative" image.

A simple technique for the preservation of vital dyes in fixed and sectioned embryos

Development ◽  
1966 ◽  
Vol 15 (2) ◽  
pp. 131-132
Author(s):  
J. Pertusa
Keyword(s):  
Methylene Blue ◽  
Ethyl Alcohol ◽  
Toluidine Blue ◽  
Trypan Blue ◽  
Simple Technique ◽  
Nile Blue ◽  
Neutral Red ◽  
The Other ◽  
Serial Sectioning ◽  
Vital Dyes

In embryological work using vital dyes it is highly desirable to be able to study the distribution of the dyes in fixed material, whether examined in toto or after embedding in paraffin and serial sectioning. However, both fixation and dehydration present problems for the preservation of colour in vitally stained cells. Some fixatives preserve some dyes but, so far as I am aware, none will preserve all the vital dyes in common use. On the other hand, ethyl alcohol destroys or dissolves all vital dyes and its use in dehydration is thus undesirable. Among the fixatives that have been proposed are those of Golowin (1902), Mitamura (1923), Parat & Painlevé (1925), and Tchéou Tai Chuin (1930) for neutral red; that of Izquierdo (1955) for toluidine blue; that of Gérard (1925) for Trypan blue; that of Turchini (1919) for methylene blue; that of Lehmann (1929) for Nile blue.

The relationship of chemical structure to teratogenic activity among bisazo dyes: a re-evaluation

Development ◽  
1966 ◽  
Vol 16 (1) ◽  
pp. 29-39
Author(s):  
J. B. Lloyd ◽  
F. Beck
Keyword(s):  
Methylene Blue ◽  
Trypan Blue ◽  
Chemical Structure ◽  
Biological Effects ◽  
Negative Results ◽  
Sudan Iv ◽  
Relationship Of ◽  
The Relationship

Although the potency of trypan blue as a teratogenic agent in rodents has been known for almost twenty years the mechanism of its action remains obscure. The dye produces a variety of biological effects (see review by Beck & Lloyd, 1966) and most of these have at some time been suggested as possibly relevant to its teratogenicity. The investigation of such correlations would clearly be assisted by determining whether the biological effects of the dye are individually modified by small changes in its chemical structure, particularly if these changes also result in differences in its teratogenicity. The first teratological study of dyes related to trypan blue was made by Gillman, Gilbert, Spence & Gillman (1951), who obtained negative results in rats with six dyes (methylene blue, trypan red, Sudan IV, Bismarck brown, Niagara blue and sky blue), but gave no experimental details of their investigation.

Intravenous Methylene Blue: a New Method for Studying the Nervous System

Nature ◽  
1948 ◽  
Vol 161 (4087) ◽  
pp. 318-319 ◽  
Author(s):  
W. H. FEINDEL ◽  
D. C. SINCLAIR ◽  
G. WEDDELL
Keyword(s):  
Methylene Blue ◽  
Nervous System ◽  
New Method

Improvement on Determination of the Adsorption Value of Activated Carbon on Methylene Blue

2014 ◽  
Vol 1033-1034 ◽  
pp. 579-582
Author(s):  
Zhao Long Huang ◽  
Shao Ping Feng ◽  
Yan Jiang ◽  
Rui Min Xiao ◽  
Li Da Sun ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Activated Carbon ◽  
Difference Method ◽  
Phosphate Buffer ◽  
Total Concentration ◽  
Phosphate Buffer Solution ◽  
New Method ◽  
Improved Method ◽  
Buffer Solution

Characteristics of absorption of activated carbon on methylene blue were studied. It was demonstrated that adsorption of activated carbon on methylene blue was very stable on monomolecular adsorption and when multimolecular adsorption existed adsorption became to be weak. An improved method was suggested that many samples were tested adsorption of activated carbon on methylene blue in parallel experiment and certain sample was selected to use in next experiment, its color should approximate to the color of 0.4% CuSO4 but darker slightly and activated carbon loaded methylene blue was added some fresh phosphate buffer solution to desorb methylene blue on the activated carbon, testing absorption value total concentration of methylene blue in the solutionin (merging the first and second experiment) by spectrophotometer, calculating adsorption value on difference method which original volume of methylene blue added subtracts rest of methylene blue in the solution after adsorption. The new method was simple, rapid and easy. word times would shorten obviously.

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