Electrochemical Biosensor of Glucose Based on the Immobilization of Glucose Oxidase on a Carbon Microsphere Modified Glassy Carbon Electrode

2012 ◽  
Vol 10 (3) ◽  
pp. 1024-1030 ◽  
Author(s):  
Yong Ping Dong ◽  
Li Huang ◽  
Zhi Ping Lei ◽  
Xiang Feng Chu ◽  
Qian Feng Zhang
Keyword(s):  
Glucose Oxidase ◽  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Electrochemical Biosensor ◽  
Carbon Electrode ◽  
Modified Glassy Carbon Electrode ◽  
Carbon Microsphere

scholarly journals Glucose Oxidase Immobilized on a Functional Polymer Modified Glassy Carbon Electrode and Its Molecule Recognition of Glucose

Polymers ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 115 ◽  
Author(s):  
Yan-Na Ning ◽  
Bao-Lin Xiao ◽  
Nan-Nan Niu ◽  
Ali Moosavi-Movahedi ◽  
Jun Hong
Keyword(s):  
Carbon Nanotubes ◽  
Electron Transfer ◽  
Glucose Oxidase ◽  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Carbon Electrode ◽  
Modified Glassy Carbon Electrode ◽  
Functional Polymer ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

In the present study, a glucose oxidase (GluOx) direct electron transfer was realized on an aminated polyethylene glycol (mPEG), carboxylic acid functionalized multi-walled carbon nanotubes (fMWCNTs), and ionic liquid (IL) composite functional polymer modified glassy carbon electrode (GCE). The amino groups in PEG, carboxyl groups in multi-walled carbon nanotubes, and IL may have a better synergistic effect, thus more effectively adjust the hydrophobicity, stability, conductivity, and biocompatibility of the composite functional polymer film. The composite polymer membranes were characterized by cyclic voltammetry (CV), ultraviolet-visible (UV-Vis) spectrophotometer, fluorescence spectroscopy, electrochemical impedance spectroscopy (EIS), and transmission electron microscopy (TEM), respectively. In 50 mM, pH 7.0 phosphate buffer solution, the formal potential and heterogeneous electron transfer constant (ks) of GluOx on the composite functional polymer modified GCE were −0.27 V and 6.5 s−1, respectively. The modified electrode could recognize and detect glucose linearly in the range of 20 to 950 μM with a detection limit of 0.2 μM. The apparent Michaelis-Menten constant (Kmapp) of the modified electrode was 143 μM. The IL/mPEG-fMWCNTs functional polymer could preserve the conformational structure and catalytic activity of GluOx and lead to high sensitivity, stability, and selectivity of the biosensors for glucose recognition and detection.

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