Synthesis of Novel Biomaterial for the Treatment of Sepsis in Rats

2020 ◽  
Vol 12 (8) ◽  
pp. 1175-1183
Author(s):  
Jia Zhou ◽  
Kai Liu ◽  
Zhilu Sun ◽  
Tao Wang
Keyword(s):  
Myocardial Protection ◽  
Ph Value ◽  
Acylation Reaction ◽  
Ammonia Water ◽  
Myocardial Apoptosis ◽  
Technological Improvement ◽  
Apoptosis Index ◽  
Chloride Method ◽  
Crystallization Stage ◽  
Surgical Group

In this research, glutamine (GLN) and L-Tyrosine were synthesized into AL-GLN synthetic biomaterials to explore their protective effect on the myocardium of sepsis symptoms. In the process of preparation, acyl chloride method was used to generate, which was generated by acylation reaction and ammonolysis reaction. In the ammonolysis reaction, the original ammonia water reaction was replaced by the ammonia water/dimethylformamide (DMF) system. The biomaterial was characterized, and it was confirmed that the product recovery rate could be maximized by adjusting the pH value to 9 during crystallization stage, and the purity could be increased from 94.23% to 98.45% after some technological improvement. During the experiment of myocardial protection in sepsis, 60 SPF rats were selected for the experiment and divided into three groups, namely the unmodified surgery group, the GLN group and the AL-GLN group. Rats with sepsis were obtained by cecum ligation and perforation. The myocardial apoptosis index and the gene expression of Bcl-2/p53mRNA were detected at 6 h, 12 h and 24 h after operation in different groups. The results showed that the expression of Bcl-2mRNA in the surgical group was significantly down-regulated compared with that in the surgical group (P < 0.05), the expression of Bcl-2mRNA in the GLN group was significantly increased compared with that in the surgical group (P < 0.05), and the expression of p53mRNA in the AL-GLN group was first decreased and then slowly increased compared with that in the surgical group (P < 0.05).

scholarly journals PL-011 Astaxanthin Reduces High Intensity Training Induced Myocardial Cell Apoptosis Via Activating Nrf2 in Rats

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Xian Guo ◽  
Jianmin Cao ◽  
Yi Wang ◽  
Haitao Zhou ◽  
Jing Zhang ◽  
...  
Keyword(s):  
High Intensity ◽  
Training Group ◽  
Myocardial Cell ◽  
Male Rats ◽  
Intensive Training ◽  
High Intensity Training ◽  
Myocardial Apoptosis ◽  
Apoptosis Index ◽  
Nrf2 Signaling Pathway ◽  
Intensity Training

Objective Long-term intensive training may led to ischemia oxygen reaction and increase the ROS. Astaxanthin, as the super antioxidant, was investigated to against anti-oxidative stress. By supplementing the astaxanthin, we wanted to observe if it can mediated Nrf2 reduces myocardial cell oxidative injury in rats after high intensity training of 6 weeks.  Methods 7-week SD male rats were divided into 3 groups randomly: control group ( C group，n =10)，high intensity training group ( HT group，n = 15)，astaxanthin and high intensity training group (HTA group，n = 15) . The rats in HTA group were given with astaxanthin 20 mg /kg·d and in HT group were given with oil during the training day．The serum cTnI，myocardial apoptosis index,  the expression of myocardial BAX, Bcl2, Nrf2, HO-1, myocardial MDA，SOD and T- AOC activity were measured 24 hours after the last training． Results After 6-week tranning of high intensity, compared with group C, the serum cTNI, myocardial apoptosis index, the expression of BAX and myocardial MDA were significantly higher in group HT(P<0.01).The Bcl2/Bax, the expression of HO-1, SOD and T-AOC activity were significantly declined (P<0.01). After the intervention of 6-week astaxanthin, compared with group HT, the serum cTNI, myocardial MDA, the myocardial apoptosis index, the expression of BAX were significantly lower in HTA group (cTNI(ng/ml): 1.16±0.27 VS 2.47±0.39, P<0.05; myocardial apoptosis index: 164.27±3.98 VS 196.20±9.65, P<0.01; BAX: 58.40±5.95 VS 78.03±3.80, P<0.01 ). Finally, Bcl2/Bax, SOD, T-AOC activity, the expression of Nrf2 and HO-1 were significantly higher  (Bcl2/Bax : 1.92±0.10 VS 1.19±0.18, P<0.01; SOD(U/mg): 52.38±6.15 VS 38.32±3.36, P<0.01; T-AOC(U/mg): 30.22±4.07 VS 23.76±3.20, P<0.01; Nrf2: 93.61±8.53 VS 74.26±6.69, P<0.01; HO-1: 84.99±13.78 VS 64.22±11.39, P<0.05).  Conclusions The supplement of astaxanthin can mediate Nrf2 signaling pathway, and elevate the expression of Nrf2 and HO-1. Then it can increase the activity of SOD and T-AOC and reduce the myocardial oxidative level and myocardial apoptosis in rats caused by 6-week high intensity training. Finally, the structure and function of heart tissue are back to normal.

β-Ni(OH)2 Flower-Like Spheres and Nanoflakes Synthesized Using the Hydrothermal Method and its Formation Mechanism

2011 ◽  
Vol 306-307 ◽  
pp. 1430-1434
Author(s):  
Xiao Ming Fu ◽  
Zhao Wen Liu ◽  
Liang Yi Zhou ◽  
Chen Chen Xie
Keyword(s):  
High Temperature ◽  
Reaction Time ◽  
Low Temperature ◽  
Hydrothermal Method ◽  
Formation Mechanism ◽  
Phase Structure ◽  
Ph Value ◽  
Nickel Nitrate ◽  
Ammonia Water

β-Ni(OH)2 flower-like spheres and nanoflakes were synthesized with strong ammonia water as precipitator and nickel nitrate as nickel source by the hydrothermal method. The phase structure and morphologies were analyzed using XRD and SEM. The results showed that under the same conditions of the pH value and the reaction time, the low temperature was propitious to synthesize β-Ni(OH)2 flower-like spheres. High temperature was in favor of the synthesis of β-Ni(OH)2 nanoflakes. Namely, β-Ni(OH)2 flower-like spheres were obtained at 180 °C for 48.0 h with pH=9.0. β-Ni(OH)2 nanoflakes were prepared at 240 °C for 48.0 h with pH=9.0. The formation mechanism was explored through observing influence of the conditions of the hydrothermal method on the morphologies of the samples.

The new St Thomas' Hospital polarized cardioplegia: improved efficacy of myocardial protection in pigs on CPB

2014 ◽  
Vol 62 (S 01) ◽  
Author(s):  
D. Santer ◽  
W. Dietl ◽  
K. Trescher ◽  
M. Kreibich ◽  
E. Dzilic ◽  
...  
Keyword(s):  
Myocardial Protection

Comparative Labelling and Biokinetic Studies of 99mTc-EDTA(Sn) and 99mTc-DTPA(Sn)

1977 ◽  
Vol 16 (01) ◽  
pp. 30-35 ◽  
Author(s):  
N. Agha ◽  
R. B. R. Persson
Keyword(s):  
Complex Formation ◽  
Significant Influence ◽  
Room Temperature ◽  
Ph Value ◽  
Maximum Activity ◽  
Reduction Step ◽  
Labelling Yield ◽  
Different Temperatures ◽  
Kinetics Of

SummaryGelchromatography column scanning has been used to study the fractions of 99mTc-pertechnetate, 99mTcchelate and reduced hydrolyzed 99mTc in preparations of 99mTc-EDTA(Sn) and 99mTc-DTPA(Sn). The labelling yield of 99mTc-EDTA(Sn) chelate was as high as 90—95% when 100 μmol EDTA · H4 and 0.5 (Amol SnCl2 was incubated with 10 ml 99mTceluate for 30—60 min at room temperature. The study of the influence of the pH-value on the fraction of 99mTc-EDTA shows that pH 2.8—2.9 gave the best labelling yield. In a comparative study of the labelling kinetics of 99mTc-EDTA(Sn) and 99mTc- DTPA(Sn) at different temperatures (7, 22 and 37°C), no significant influence on the reduction step was found. The rate constant for complex formation, however, increased more rapidly with increased temperature for 99mTc-DTPA(Sn). At room temperature only a few minutes was required to achieve a high labelling yield with 99mTc-DTPA(Sn) whereas about 60 min was required for 99mTc-EDTA(Sn). Comparative biokinetic studies in rabbits showed that the maximum activity in kidneys is achieved after 12 min with 99mTc-EDTA(Sn) but already after 6 min with 99mTc-DTPA(Sn). The long-term disappearance of 99mTc-DTPA(Sn) from the kidneys is about five times faster than that for 99mTc-EDTA(Sn).

Rhenium-188 Labeled Hydroxyapatite and Rhenium-188 Sulfur Colloid

1997 ◽  
Vol 36 (02) ◽  
pp. 71-75 ◽  
Author(s):  
S. Glatz ◽  
S. N. Reske ◽  
K. G. Grillenberger
Keyword(s):  
Synovial Fluid ◽  
Ph Value ◽  
Surgical Removal ◽  
Radiation Synovectomy ◽  
Sulfur Colloid ◽  
Target Organs ◽  
Aseptic Conditions ◽  
In Vitro Stability ◽  
Potential Use

Summary Aim: One therapeutic approach to rheumatoid arthritis and other inflammatory arthropathies besides surgical removal of inflamed synovium is radiation synovectomy using beta-emitting radionuclides to destroy the affected synovial tissue. Up to now the major problem associated with the use of labeled particles or colloids has been considerable leakage of radionuclides from the injected joint coupled with high radiation doses to liver and other non target organs. In this study we compared 188Re labeled hydroxyapatite particles and 188Re rhenium sulfur colloid for their potential use in radiation synovectomy. Methods: To this end we varied the labeling conditions (concentrations, pH-value, heating procedure) and analyzed the labeling yield, radiochemical purity, and in vitro stability of the resulting radiopharmaceutical. Results: After optimizing labeling conditions we achieved a labeling yield of more than 80% for 188Re hydroxyapatite and more than 90% for the rhenium sulfur colloid. Both of the radiopharmaceuticals can be prepared under aseptic conditions using an autoclav for heating without loss of activity. In vitro stability studies using various challenge solutions (water, normal saline, diluted synovial fluid) showed that 188Re labeled hydroxyapatite particles lost about 80% of their activity within 5 d in synovial fluid. Rhenium sulfur colloid on the other hand proved to be very stable with a remaining activity of more than 93% after 5 d in diluted synovial fluid. Conclusion: These in vitro results suggest that 188Re labeled rhenium sulfur colloid expects to be more suitable for therapeutic use in radiation synovectomy than the labeled hydroxyapatite particles.

Influence of saliva pH-value on the ratio of amphetamine saliva to serum concentration

2019 ◽  
Author(s):  
C Wohkittel ◽  
M Romanos ◽  
A Diehl ◽  
P Högger ◽  
M Gerlach
Keyword(s):  
Serum Concentration ◽  
Ph Value

DIP quality influenced by process water quality

TAPPI Journal ◽  
2014 ◽  
Vol 13 (9) ◽  
pp. 51-60
Author(s):  
DENNIS VOSS ◽  
HANS-JOACHIM PUTZ ◽  
SAMUEL SCHABEL
Keyword(s):  
Water Quality ◽  
Surface Tension ◽  
Ph Value ◽  
Water Hardness ◽  
Process Water ◽  
Test Results ◽  
Negative Effects ◽  
Water Parameters ◽  
Effects Of Ph ◽  
Contamination Levels

The need for deinking mills to reduce their fresh water consumption has resulted in higher loads of various contaminants in the process water. Lower recovered paper quality also leads to higher contamination levels in the mills. This higher load has an influence on achievable target brightness. The objective of the work was to determine and explain the main reasons for relatively poor deinked pulp quality or poor deinking potential based on the influence of recovered paper composition and process water quality. The process water parameters significantly affect the deinking potential of recovered paper. The test results showed the negative effects of increased water hardness. For standard recovered paper mixtures, flotation selectivity is higher with increasing flotation pH-value. Good results were realized for standard recovered paper with low hardness, low surface tension, and high pH-value. The results for recovered paper containing flexo newsprint could be slightly improved with low hardness, low surface tension, and low pH-value. The results of the test program using design of experiments showed interacting effects of pH-value and surface tension on luminosity and flotation selectivity.

Kualitas Fisik Daging Ayam Broiler yang Diberi Minyak Buah Merah dalam Pakan Komersial

2019 ◽  
Vol 6 (1) ◽  
pp. 20
Author(s):  
Abdul Rahman Ollong ◽  
Rizki Arizona ◽  
Rusli Badaruddin
Keyword(s):  
Moisture Content ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Ph Value ◽  
Cooking Loss ◽  
Water Holding Capacity ◽  
Broiler Meat ◽  
Fruit Oil ◽  
Water Holding ◽  
Range Test

ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh penambahan minyak buah merah (MBM) pada pakan terhadap kualitas fisik daging ayam broiler. Seratus ekor ayam broiler umur sehari (DOC) ditempatkan pada lima kelompok perlakuan pakan yang berbeda, yaitu: P1 (pakan kontrol/tanpa penambahan minyak), P2 (2% MBM), P3 (4% MBM), P4 (6% MBM) dan P5 (6% Minyak kelapa sawit). Setiap kelompok perlakuan terdiri dari empat ulangan masing-masing dengan lima ekor. Ayam broiler dipelihara selama 35 hari. Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan analisis variansi pola searah dan diuji lanjut dengan Duncan’s New Multiple Range Test (DMRT). Hasil penelitian menunjukkan bahwa semua variable yang diamati menunjukkan adanya pengaruh nyata (P<0,05) terhadap perlakuan yang diberikan. Dari hasil penelitian ini dapat disimpulkan bahwa penggunaan minyak buah merah (MBM) dalam pakan mampu memberikan pengaruh terhadap pH Daging, Daya Ikat Air (DIA), susut masak dan keempukan daging ayam broiler.Kata kunci : daging ayam broiler, daya ikat air, keempukan daging, pH daging, susut masakABSTRACT The experiment was conducted to study the effect of red fruit oil (RFO) onphysical quality  of broiler chicken. One hundred day old chicken (DOC) were placed in four groups of different treatments, of from levels of RFO (P1 (diet without addition of RFO), P2 (2% RFO), P3 (4% RFO) and P4 (6% RFO) and P5 (6% Palm oil)). The treatment group consisted of fivereplications with five birds each. Broiler chickens were reared for 35 days. Statistical analysis used Completely Randomized Design (CRD) and followed by Duncan’s New Multiple Range Test (DMRT). The results showed that the pH value, moisture content, water holding capacity, and cooking loss was significant differences. It could be concluded that the addition of red fruit oil in the diet give effect  of broiler chicken meat.Keywords: broiler meat, cooking loss, moisture content, pH value, water holding capacity

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