Diagnosis of Oral Squamous Cell Carcinoma (OSCC) Using Laser Induced Fluorescence

2020 ◽  
Vol 12 (6) ◽  
pp. 853-860 ◽  
Author(s):  
M. A. N. Razvi ◽  
Ahmed Bakry ◽  
A. Saeed ◽  
S. M. Afzal ◽  
Y. F. AL-Hadeethi ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Continuous Wave ◽  
Laser Induced Fluorescence ◽  
Significant Shift ◽  
Tissue Samples ◽  
Ffpe Tissues ◽  
Lif Spectroscopy

Cancer is a dreaded disease; a large number of deaths occur every year due to this disease. Oral squamous cell carcinoma (OSCC) is the most common cancer of the head and neck, which is approximately 16% to 40% of all malignancies. In this study, Laser induced fluorescence (LIF) spectroscopy has been utilized to discriminate OSCC against healthy (normal) tissues and to investigate whether the LIF could provide information from formalin-fixed paraffin-embedded (FFPE) tissue samples similar to that reported using fresh tissues. Samples were prepared after biopsy from ten patients using standard FFPE tissues methods. LIF system consists of a continuous wave (CW) He–Cd laser at 325 nm, a seven-core optical fiber cable coupled to the laser, a spectrometer with cooled charge coupled device (CCD) detector, and a computer for acquisition of the LIF spectra. Spectra were decomposed using second derivative and curve fitting analysis to reveal the changes in molecular composition of the samples. Moreover, samples spectra were discriminated by hierarchical cluster analysis (HCA) and principal components analysis (PCA). Spectral results showed differences in peak areas and positions between normal and OSCC tissues. LIF spectroscopy revealed significant decrease in the peak area of collagen and decrease in peak area of coenzymes of OSCC tissues. In addition, significant shift in the peak position of coenzymes was recorded. HCA and PCA of LIF indicated a very clear discrimination of the normal and FFPE-OSCC tissues. The achieved discrimination between elliptic polygons of normal and OSCC tissues was 96.3% by PCA. This study confirms that the LIF spectroscopy is a good diagnostic tool for OSCC and it could be used with samples that are prepared using FFPE tissues methods.

scholarly journals Expression of RAD51 and Its Clinical Impact in Oral Squamous Cell Carcinoma

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Yuyang Li ◽  
Jia Li ◽  
Jingchun Sun ◽  
Yingkun Liu ◽  
Dingkun Liu ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Normal Skin ◽  
Clinical Stage ◽  
Prognostic Indicator ◽  
Normal Mucosa ◽  
Strong Positive Correlation ◽  
Tissue Samples

Purpose. To examine the expression of RAD51 in oral squamous cell carcinoma (OSCC) and analyze its connection with pathological grade, clinical stage, and lymphatic metastasis potential. Methods. For this study, 74 OSCC samples, 15 normal mucosa tissues, and 11 normal skin tissue samples were collected. RAD51 expression was investigated using immunohistochemistry. A follow-up visit was used to assess the prognosis of each patient. We compared RAD51 expression in oral mucosa epithelial cells (OMECs), keratinocytes, and tongue squamous cell carcinoma cells (TSCCs) by Western blot analysis. Results. RAD51 expression was higher in tumor cells than in normal mucosal tissues. In addition, RAD51 expression was associated with higher tumor differentiation (P<0.05). Also, RAD51 expression was higher (P<0.05) in patients with lymphatic metastases, and relapse rates were also higher in patients with elevated RAD51 levels (P=0.052). In addition, RAD51 expression levels were highest in the skin keratinocytes, followed by the TSCCs and OMECs. Conclusion. A strong positive correlation was found between RAD51 expression and the degree of malignancy in OSCC patients, suggesting that RAD51 could be an excellent prognostic indicator for OSCC patients.

scholarly journals Application of the eighth edition of the American joint committee on cancer staging system for oral carcinoma

2019 ◽  
Vol 72 (5-6) ◽  
pp. 165-170
Author(s):  
Ivana Mijatov ◽  
Sasa Mijatov
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Maxillofacial Surgery ◽  
Staging System ◽  
Nodal Status ◽  
Oral Carcinoma ◽  
Significant Shift ◽  
8Th Edition

Introduction. Oral squamous cell carcinoma is the sixth most common carcinoma in the world. Annually, it accounts for 5% of all newly discovered cancers. The most important prognostic factor is the stage of the disease. The tumor, node, and metastasis staging system has been the cornerstone for clinical classification of oral squamous cell carcinoma. Material and Methods. The study included 65 patients with oral squamous cell carcinoma who underwent surgery at the Clinic of Maxillofacial Surgery of the Clinical Center of Vojvodina in Novi Sad. The tumor, node, and metastasis status was determined according to 7th and 8th edition of the tumor, node, and metastasis classification. Results. Statistical differences between the 7th and 8th edition of tumor, node, and metastasis classification were examined. There was also a change in the nodal status; in 20% of patients there was a transition from N1 to N2, as a result of a more precise definition of nodal status in patients with oral carcinoma. Conclusion. This research has pointed out the significance of tumor size as a predictive factor in oral squamous cell carcinoma, which indicates the importance of its local control (for surgical and radiological treatment). The 8th edition of the tumor, node, and metastasis classification for oral cavity cancers made a significant shift by clearly defining depth of tumor invasion into the tumor status.

scholarly journals Gene expression of one important microRNA in blood and tissue samples of oral squamous cell carcinoma

2021 ◽  
Author(s):  
Naghmeh Emami ◽  
Naghmeh Bahrami ◽  
Masoumeh Mirzaei ◽  
Abdolreza Mohamadnia
Keyword(s):  
Gene Expression ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Peripheral Blood ◽  
Expression Level ◽  
P Value ◽  
Healthy Individuals ◽  
Tissue Samples

Introduction: Oral Squamous Cell Carcinoma (OSCC) is one of the most common oral malignancies, which accounts for 80-90% of malignant neoplasms of the oral cavity. MicroRNAs (miRNAs) are small RNA molecules that regulate post-transcriptional gene expression by targeting mRNAs. Materials and Methods: In this case-control study, 40 patients with oral squamous cell carcinoma and 40 healthy individuals as control were studied. Blood samples were collected from both groups. Also, 30 cancer tissue samples and 30 healthy tissue samples were prepared and evaluated. RNA was extracted from collected peripheral blood and tissue samples and evaluated for the expression level of miR-494 via real-time PCR technique. P. value values<0.05 were considered statistically significant. Results: The expression level of miR-494 in serum (peripheral blood) of patients with oral squa- mous cell carcinoma increased by 1.12 fold (P-value<0.001) compared with healthy individuals. Also, the expression level of miR-494 in samples of oral squamous cell carcinoma infected tissue showed a 1.28-fold increase compared to healthy tissue. Conclusion: The results of this study indicate an increase in the expression level (up-regula- tion) of miR-494 in oral squamous cell carcinoma. This biomarker can be used in screening and early detection of oral squamous cell carcinoma.

scholarly journals Follow-up of collagen crosslink excretion in patients with oral squamous cell carcinoma and analysis of tissue samples

2003 ◽  
Vol 89 (9) ◽  
pp. 1722-1728 ◽  
Author(s):  
I N G Springer ◽  
H Terheyden ◽  
M A A Suhr ◽  
P Warnke ◽  
A Dunsche ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Tissue Samples

scholarly journals Promoter Region Hypermethylation and mRNA Expression ofMGMTandp16Genes in Tissue and Blood Samples of Human Premalignant Oral Lesions and Oral Squamous Cell Carcinoma

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Vikram Bhatia ◽  
Madhu Mati Goel ◽  
Annu Makker ◽  
Shikha Tewari ◽  
Alka Yadu ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Mrna Expression ◽  
Squamous Cell ◽  
Promoter Methylation ◽  
Dna Methyltransferase ◽  
Oral Lesions ◽  
Tissue Samples ◽  
Blood Samples

Promoter methylation and relative gene expression of O6-methyguanine-DNA-methyltransferase (MGMT) andp16genes were examined in tissue and blood samples of patients with premalignant oral lesions (PMOLs) and oral squamous cell carcinoma (OSCC). Methylation-specific PCR and reverse transcriptase PCR were performed in 146 tissue and blood samples from controls and patients with PMOLs and OSCC. In PMOL group, significant promoter methylation ofMGMTandp16genes was observed in 59% (P=0.0010) and 57% (P=0.0016) of tissue samples, respectively, and 39% (P=0.0135) and 33% (P=0.0074) of blood samples, respectively. Promoter methylation of both genes was more frequent in patients with OSCC, that is, 76% (P=0.0001) and 82% (P=0.0001) in tissue and 57% (P=0.0002) and 70% (P=0.0001) in blood, respectively. Significant downregulation ofMGMTandp16mRNA expression was observed in both tissue and blood samples from patients with PMOLs and OSCC. Hypermethylation-induced transcriptional silencing ofMGMTandp16genes in both precancer and cancer suggests important role of these changes in progression of premalignant state to malignancy. Results support use of blood as potential surrogate to tissue samples for screening or diagnosing PMOLs and early OSCC.

Evaluation of MCM7 And P16 INK4 Expression in Tissue Samples From Patients With Oral Squamous Cell Carcinoma (OSCC)

2014 ◽  
Vol 117 (2) ◽  
pp. e206
Author(s):  
ZELINA LISLEY PEREIRA ◽  
ANNAMARIA RAVARA VAGO ◽  
FRANCISCO BERNARDO ARREGUY DE OLIVEIRA ◽  
MARCELO VIDIGAL CALIARI ◽  
RICARDO SANTIAGO GOMEZ ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Tissue Samples

scholarly journals Expression of MAGE-A12 in Oral Squamous Cell Carcinoma

2008 ◽  
Vol 24 (1) ◽  
pp. 27-32 ◽  
Author(s):  
Nur Mollaoglu ◽  
Eleftherios Vairaktaris ◽  
Emeka Nkenke ◽  
Friedrich W. Neukam ◽  
Jutta Ries
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cancer Cells ◽  
Squamous Cell ◽  
Malignant Lesion ◽  
Nested Polymerase Chain Reaction ◽  
Tissue Samples ◽  
Normal Tissues ◽  
Expression Rate

Melanoma associated-A antigens (MAGE-A) are silent in normal tissues except testis. However, they are activated in a variety of different tumors. Thus, their expression is highly specific to cancer cells. Reverse transcription-nested polymerase chain reaction (RT-nPCR) is a highly sensitive technique that has been used successfully for the detection ofMAGE genes in tissue samples. The aim of the study is to analyze the expression rate of MAGE-A12 in oral squamous cell carcinoma (OSCC) using a high sensitive RT-nPCR. Total of 57 tissue samples obtained from patients with OSCC and 20 normal oral mucosal (NOM) probes of otherwise healthy volunteers were included to this study. No expression of MAGE-A12 was observed in the non-neoplastic NOM tissues. MAGE-A12 was expressed in 49.1% of the investigated tumor samples. The correlation between malignant lesion and MAGE-A12 detection was significant (p < 0.001). It is concluded that results of this study may indicate MAGE-A12 as a useful additional diagnostic marker especially for the early detection of OSCC distinguishing neoplastic transformation and detection of occult and/or rare disseminated cancer cells. In addition, MAGE-A12 expression in OSCC may also determine a new immunotherapeutic target and might be warranted to develop vaccine for OSCC.

scholarly journals Long Non-Coding RNA LINC01929 Accelerates Progression of Oral Squamous Cell Carcinoma by Targeting the miR-137-3p/FOXC1 Axis

2021 ◽  
Vol 11 ◽  
Author(s):  
Hongze Che ◽  
Yanhai Che ◽  
Zhimin Zhang ◽  
Qing Lu
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cell Lines ◽  
Squamous Cell ◽  
Cell Counting ◽  
Migration And Invasion ◽  
Tissue Samples ◽  
Non Coding Rna ◽  
Luciferase Activity Assay

Recently, additional long noncoding RNAs (lncRNAs) have been identified and their possible roles were investigated in a variety of human tumors. One of these lncRNAs, LINC01929, promoted the progression of some cancers, whereas its expression and biological function in human oral squamous cell carcinoma (OSCC) remains still mostly uncertain. The LINC01929 expression in OSCC tissues or cell lines was identified via quantitative real-time polymerase chain reaction. The cell counting kit-8, transwell migration, wound-healing, and flow cytometry assays were utilized to characterize the functions of LINC01929 in OSCC cells. The interactive relationships between LINC01929 and miR-137-3p, miR-137-3p and Forkhead box C1 (FOXC1) were investigated by the dual-luciferase activity assay. Our findings demonstrated that LINC01929 was highly expressed in OSCC tissue samples and cell lines, whereas miR-137-3p expression was downregulated. LINC01929 acted as a carcinogenic lncRNA with accelerated OSCC cell proliferation, migration and invasion, and suppression of apoptosis. We further indicated that LINC01929 facilitated tumor growth in xenograft mouse models. Mechanistically, LINC01929 acted as a sponge for miR-137-3p to elevate FOXC1 expression, which is the target of miR-137-3p. In addition, downregulated miR-137-3p expression rescued the suppressive behaviors of LINC01929 knockdown on the biological behaviors of OSCC cells. Taken together, LINC01929 functioned as a tumor-promoting lncRNA via the miR-137-3p/FOXC1 axis in OSCC, suggesting novel targets for OSCC therapy.

scholarly journals Desmosomal Component Expression in Normal, Dysplastic, and Oral Squamous Cell Carcinoma

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Nagamani Narayana ◽  
Julie Gist ◽  
Tyler Smith ◽  
Daniel Tylka ◽  
Gavin Trogdon ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Cancer ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Oral Epithelium ◽  
Tissue Samples ◽  
Altered Expression ◽  
Cellular Change ◽  
Premalignant Disease

Squamous cell carcinoma (oral SCC) is the most common oral cancer in the U.S., affecting nearly 30,000 Americans each year. Despite recent advances in detection and treatment, there has been little improvement in the five-year survival rate for this devastating disease. Oral cancer may be preceded by premalignant disease that appears histologically as dysplasia. Identification of molecular markers for cellular change would assist in determining the risk of dysplasia progressing to oral squamous cell carcinoma. The goal of this study was to determine if any correlation exists between histological diagnosed dysplasia and OSCC lesions and altered expression of desmosomal cell-cell adhesion molecules in the oral epithelium. Our data showed that oral SCC tissue samples showed decreased immunoreactivity of both desmoplakin and plakophilin-1 proteins compared to normal oral epithelium. Furthermore, significant decrease in desmoplakin immunoreactivity was observed in dysplastic tissue compared to normal oral epithelium. In contrast, the level of desmoglein-1 staining was unchanged between samples however desmoglein-1 was found localized to cell borders in oral SCC samples. These data suggest that changes in expression of desmoplakin and plakophilin-1 may prove to be a useful marker for changes in tissue morphology and provide a tool for identifying pre-neoplastic lesions of the oral cavity.

scholarly journals Clinical Significance and Prognostic Value of the Expression of LAMP3 in Oral Squamous Cell Carcinoma

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Jun Lu ◽  
Hengcheng Ma ◽  
Shuijin Lian ◽  
Dan Huang ◽  
Min Lian ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Prognostic Marker ◽  
Squamous Cell ◽  
Biological Activities ◽  
Tissue Samples ◽  
Protein Levels ◽  
Normal Tissues ◽  
Chi Square Analysis

Recent studies demonstrated high expression of lysosome-associated membrane protein 3 (LAMP3) in a variety of malignancies including esophageal squamous cell carcinoma, gastrointestinal cancer, breast cancer, and cervical cancer and its involvement in several biological activities of tumor cells. However, the expression of LAMP3 and its value in oral squamous cell carcinoma (OSCC) remain unclear. In this study, we examined the expression of LAMP3 in OSCC tissue samples and investigated the relationship between LAMP3 and clinical characteristics of patients with OSCC. We examined mRNA and protein levels of LAMP3 in OSCC tissues and neighboring normal tissues using quantitative real-time polymerase chain reaction and immunohistochemistry analyses, respectively. Both the mRNA and protein levels of LAMP3 were significantly higher in OSCC tissues than in adjacent normal tissues. Chi-square analysis showed that the high LAMP3 expression was notably linked to the degree of tumor differentiation and advanced TNM stage. Univariate and multivariate analyses showed that the high LAMP3 expression was an independent prognostic marker in OSCC. Our results suggest that LAMP3 might act as a potential anticancer target and a prognostic marker in patients with OSCC.

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