Detection and Imaging Application of miRNA in Cells and Living Organisms with Nano-Fluorescent Probes Made by Novel Synthesis Materials

2019 ◽  
Vol 11 (12) ◽  
pp. 1806-1815
Author(s):  
Dan Zhou ◽  
Lei Zhou ◽  
Xiaoyan Hu ◽  
Yan Hu ◽  
Ping Hu
Keyword(s):  
Rare Earth ◽  
Cancer Cells ◽  
Fluorescent Probe ◽  
Fluorescence Intensity ◽  
Fluorescent Probes ◽  
Control Group ◽  
Cobalt Oxyhydroxide ◽  
Fluorescence Reaction ◽  
Living Organisms ◽  
In Cells

As a kind of rare earth fluorescent material, the rare earth upconversion nanomaterial can be applied in various fields such as biological detection and imaging, solar cells, and safe positioning, which has attracted wide concerns. In this study, the novel material is applied to the preparation of biological nano-fluorescent probes. Due to its broad UV absorption spectrum, cobalt oxyhydroxide is selected and used as a quencher for upconversion nanomaterials. Once the cobalt oxyhydroxide is placed on upconversion nanomaterials, the surface reaction can effectively remove the fluorescence reaction of the upconversion nanomaterial. In terms of the molecular miRNA tests for cells and living organisms, the nano-fluorescent probe can reduce the fluorescence intensity of miRNA, while the control group can finish the normal fluorescence reaction. The designed fluorescent probe can adjust the contents of cobalt oxyhydroxides and cells to regulate the fluorescence intensity. In terms of the miRNA sensitivity tests, the fluorescence intensity detected by the nano-fluorescent probe is significantly lower than that in the control group, which can be observed through the fluorescence recovery tests of the chemical system. After the addition of miRNA obtained from cells or living organisms, the fluorescent probe has apparently changed the fluorescence intensity of miRNA in cells/living organisms. Also, the detection range of miRNA is effectively expanded, i.e., the different concentrations of miRNA can be detected by adjusting the ratio of the components of the fluorescent probes, which indicates the excellent sensitivity of the fluorescent probe in detecting miRNA in cells and living organisms. In terms of the miRNA tests for cells, different degrees of cancer cells are selected. The fluorescent probe can discriminate the concentration of cancer cells according to fluorescence imaging of cancer cells, thereby further explaining that the fluorescent probe has high-sensitivity in bio-detection.

A lysosome-targeted fluorescent probe for the real-time detection of hypobromous acid in living human cancer cells

The Analyst ◽  
2021 ◽  
Author(s):  
Nathaniel Finney ◽  
Yali Wang ◽  
Yuan Zhang ◽  
Lijun Yang ◽  
Huiyuan Wu
Keyword(s):  
Reactive Oxygen Species ◽  
Cancer Cells ◽  
Real Time ◽  
Fluorescent Probe ◽  
Fluorescent Probes ◽  
Human Cancer ◽  
Oxygen Species ◽  
The Real ◽  
Human Cancer Cells ◽  
Hypobromous Acid

Developing rapid, sensitive, selective fluorescent probes for HOBr detection and imaging is of great importance. It is a reactive oxygen species (ROS) with numerous biological roles: excessive or misplaced generation...

Targetable, two-photon fluorescent probes for local nitric oxide capture in the plasma membranes of live cells and brain tissues

The Analyst ◽  
2018 ◽  
Vol 143 (17) ◽  
pp. 4180-4188 ◽  
Author(s):  
Xinfu Zhang ◽  
Benlei Wang ◽  
Yi Xiao ◽  
Chao Wang ◽  
Ling He
Keyword(s):  
Nitric Oxide ◽  
Plasma Membrane ◽  
Fluorescent Probe ◽  
Fluorescent Probes ◽  
Plasma Membranes ◽  
Live Cells ◽  
Two Photon ◽  
In Cells ◽  
Brain Tissues

A plasma membrane-targetable two-photon fluorescent probe for capturing nitric oxide in cells and brain tissues.

“ON–OFF–ON” fluorescent probes based on nitrogen-doped carbon dots for hypochlorite and bisulfite detection in living cells

The Analyst ◽  
2018 ◽  
Vol 143 (23) ◽  
pp. 5834-5840 ◽  
Author(s):  
Ruoming Wang ◽  
Ruixia Wang ◽  
Dongyan Ju ◽  
Wei Lu ◽  
Chunzhu Jiang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Fluorescent Probes ◽  
Carbon Dots ◽  
Solvothermal Method ◽  
Living Cells ◽  
Nitrogen Doped ◽  
Doped Carbon Dots ◽  
Nitrogen Doped Carbon ◽  
In Cells

A fluorescent probe based on N-CDs was synthesized by a solvothermal method to detect ClO− and HSO3− in cells through an “ON–OFF–ON” mode.

Synthesis and Characterization of ROSA Dye - A Rhodamine B-type Fluorophore, Suitable for Bioconjugation and Fluorescence Studies in Live Cells

2019 ◽  
Vol 26 (10) ◽  
pp. 758-767
Author(s):  
Vicente Rubio ◽  
Vijaya Iragavarapu ◽  
Maciej J. Stawikowski
Keyword(s):  
Quantum Yield ◽  
Cancer Cells ◽  
Fluorescent Probe ◽  
Extinction Coefficient ◽  
Rhodamine B ◽  
Solid Phase ◽  
Total Yield ◽  
Molar Extinction Coefficient ◽  
Αvβ3 Integrin ◽  
Αvβ3 Integrins

Background: Herein we report the multigram-scale synthesis, characterization and application of a rhodamine B-based fluorophore (ROSA) suitable for fluorescent studies in biological applications. This fluorophore is devoid of rhodamine spirolactone formation and furthermore characterized by a high molar extinction coefficient (ϵ=87250 ± 1630 M-1cm-1) and quantum yield (φ) of 0.589 ± 0.070 in water. Reported here is also the application of ROSA towards synthesis of a ROSA-PEG-GRGDS-NH2 fluorescent probe suitable for live cell imaging of αvβ3 integrins for in vitro assays. Objective: The main objective of this study is to efficiently prepare rhodamine B derivative, devoid of spirolactone formation that would be suitable for bioconjugation and subsequent bioimaging. Methods: Rhodamine B was transformed into rhodamine B succinimide ester (RhoB-OSu) using N-hydroxysuccinimide. RhoB-OSu was further coupled to sarcosine to obtain rhodamine Bsarcosine dye (ROSA) in good yield. The ROSA dye was then coupled to a αvβ3 integrin binding sequence using standard solid-phase conditions. Resulting ROSA-PEG-GRGDS-NH2 probe was used to image integrins on cancer cells. Results: The rhodamine B-sarcosine dye (ROSA) was obtained in multigram scale in good total yield of 47%. Unlike rhodamine B, the ROSA dye does not undergo pH-dependent spirolactone/spirolactam formation as compared with rhodamine B-glycine. It is also characterized by excellent quantum yield (φ) of 0.589 ± 0.070 in water and high molar extinction coefficient of 87250 ± 1630 M-1cm-1. ROSA coupling to the RGD-like peptide was proved to be efficient and straightforward. Imaging using standard filters on multimode plate reader and confocal microscope was performed. The αvβ3 integrins present on the surface of live WM-266-4 (melanoma) and MCF- 7 (breast cancer) cells were successfully imaged. Conclusion: We successfully derivatized rhodamine B to create an inexpensive, stable and convenient to use fluorescent probe. The obtained derivative has excellent photochemical properties and it is suitable for bioconjugation and many imaging applications.

Nitrogen and Sulfur Co-doped Fluorescent Carbon Dots for the Detection of Morin and Cell Imaging

2018 ◽  
Vol 15 (1) ◽  
pp. 47-55
Author(s):  
Xuebing Li ◽  
Haifen Yang ◽  
Ning Wang ◽  
Tijian Sun ◽  
Wei Bian ◽  
...  
Keyword(s):  
Microwave Heating ◽  
Fluorescent Probe ◽  
Fluorescence Intensity ◽  
Carbon Dots ◽  
Cell Imaging ◽  
Water Soluble ◽  
Heating Process ◽  
X Ray ◽  
Doped Carbon Dots ◽  
Co Doped

Background: Morin has many pharmacological functions including antioxidant, anticancer, anti-inflammatory, and antibacterial effects. It is commonly used in the treatment of antiviral infection, gastropathy, coronary heart disease and hepatitis B in clinic. However, researches have shown that morin is likely to show prooxidative effects on the cells when the amount of treatment is at high dose, leading to the decrease of intracellular ATP levels and the increase of necrosis process. Therefore, it is necessary to determine the concentration of morin in biologic samples. Method: Novel water-soluble and green nitrogen and sulfur co-doped carbon dots (NSCDs) were prepared by a microwave heating process with citric acid and L-cysteine. The fluorescence spectra were collected at an excitation wavelength of 350 nm when solutions of NSCDs were mixed with various concentrations of morin. Results: The as-prepared NSCDs were characterized by transmission electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. The fluorescence intensity of NSCDs decreased significantly with the increase of morin concentration. The fluorescence intensity of NSCDs displayed a linear response to morin in the concentration 0.10-30 μM with a low detection limit of 56 nM. The proposed fluorescent probe was applied to analysis of morin in human body fluids with recoveries of 98.0-102%. Conclusion: NSCDs were prepared by a microwave heating process. The present analytical method is sensitive to morin. The quenching process between NSCDs and morin is attributed to the static quenching. In addition, the cellular toxicity on HeLa cells indicated that the as-prepared NSCDs fluorescent probe does not show obvious cytotoxicity in cell imaging. Our proposed method possibly opens up a rapid and nontoxic way for preparing heteroatom doped carbon dots with a broad application prospect.

scholarly journals Structure modulation on fluorescent probes for biothiols and the reversible imaging of glutathione in living cells

RSC Advances ◽  
2021 ◽  
Vol 11 (34) ◽  
pp. 21116-21126
Author(s):  
Yu Li ◽  
Li Chen ◽  
Yan Zhu ◽  
Liming Chen ◽  
Xianglin Yu ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Fluorescent Probes ◽  
Living Cells ◽  
Structure Modulation

A reversible fluorescent probe for GSH was obtained through structure modulation, by which the intracellular GSH fluctuation was imaged.

scholarly journals Amoebic Foraging Model of Metastatic Cancer Cells

Symmetry ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1140
Author(s):  
Daiki Andoh ◽  
Yukio-Pegio Gunji
Keyword(s):  
Bayesian Inference ◽  
Cancer Cells ◽  
Metastatic Cancer ◽  
Gait Patterns ◽  
Lévy Walk ◽  
Levy Walk ◽  
Unicellular Organisms ◽  
Living Organisms ◽  
Walk Algorithm ◽  
Metastatic Cancer Cells

The Lévy walk is a pattern that is often seen in the movement of living organisms; it has both ballistic and random features and is a behavior that has been recognized in various animals and unicellular organisms, such as amoebae, in recent years. We proposed an amoeba locomotion model that implements Bayesian and inverse Bayesian inference as a Lévy walk algorithm that balances exploration and exploitation, and through a comparison with general random walks, we confirmed its effectiveness. While Bayesian inference is expressed only by P(h) = P(h|d), we introduce inverse Bayesian inference expressed as P(d|h) = P(d) in a symmetry fashion. That symmetry contributes to balancing contracting and expanding the probability space. Additionally, the conditions of various environments were set, and experimental results were obtained that corresponded to changes in gait patterns with respect to changes in the conditions of actual metastatic cancer cells.

scholarly journals Sevoflurane represses the migration and invasion of gastric cancer cells by regulating forkhead box protein 3

2021 ◽  
Vol 49 (4) ◽  
pp. 030006052110059
Author(s):  
Fangfang Yong ◽  
Hemei Wang ◽  
Chao Li ◽  
Huiqun Jia
Keyword(s):  
Gastric Cancer ◽  
Cell Migration ◽  
Cancer Cells ◽  
Cell Lines ◽  
Migration And Invasion ◽  
Control Group ◽  
Gastric Cancer Cells ◽  
Cell Migration And Invasion ◽  
Forkhead Box ◽  
Induced Apoptosis

Objective Previous studies suggested that sevoflurane exerts anti-proliferative, anti-migratory, and anti-invasive effects on cancer cells. To determine the role of sevoflurane on gastric cancer (GC) progression, we evaluated its effects on the proliferation, migration, and invasion of SGC7901, AGS, and MGC803 GC cells. Methods GC cells were exposed to different concentrations of sevoflurane (1.7, 3.4, or 5.1% v/v). Cell viability, migration, and invasion were evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and Transwell assays. Immunohistochemical staining and immunoblotting were performed to analyze forkhead box protein 3 (FOXP3) protein expression in tissue specimens and cell lines, respectively. Results FOXP3 was downregulated in human GC specimens and cell lines. Functionally, FOXP3 overexpression significantly inhibited the proliferation, migration, and invasion of GC cells and accelerated their apoptosis. Moreover, sevoflurane significantly blocked GC cell migration and invasion compared with the findings in the control group. However, FOXP3 silencing neutralized sevoflurane-induced apoptosis and the inhibition of GC cell migration and invasion. Sevoflurane-induced apoptosis and the suppression of migration and invasion might be associated with FOXP3 overactivation in GC cells. Conclusions Sevoflurane activated FOXP3 and prevented GC progression via inhibiting cell migration and invasion in vitro.

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