Preparation of Functionalized Graphene Oxide Nanocomposites for Covalent Immobilization of NADH Oxidase

2016 ◽  
Vol 8 (2) ◽  
pp. 164-167 ◽  
Author(s):  
Cai-Hong Liu ◽  
Xiao-Qian Li ◽  
Xiao-Ping Jiang ◽  
Meng-Yao Zhuang ◽  
Jiu-Xun Zhang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Nadh Oxidase ◽  
Covalent Immobilization ◽  
Functionalized Graphene ◽  
Functionalized Graphene Oxide

The Amino-Functionalized Graphene Oxide Nanosheet Preparation for Enzyme Covalent Immobilization

2020 ◽  
Vol 20 (12) ◽  
pp. 7398-7405
Author(s):  
Lai Wenqing ◽  
Lin Bencheng ◽  
Zhang Wei ◽  
Yan Jun ◽  
Liu Huanliang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Catalyst Activity ◽  
Effective Means ◽  
Storage Conditions ◽  
Covalent Immobilization ◽  
Functionalized Graphene ◽  
Optimal Temperature ◽  
Functionalized Graphene Oxide ◽  
Ft Ir ◽  
Higher Temperature

Enzyme immobilization has demonstrated effective means for extending protein stability and shelf life. However, current methods negatively affect the enzyme activity, particularly for application purposes. Herein, the amino functionalized graphene oxide (GO–NH2) was synthesized and used to covalently immobilize lactase. FT-IR, UV-Vis, SEM, TEM and XPS were employed to confirm and characterize the immobilized lactase. At the resulting optimal temperature, the immobilization rate achieved 61% and the immobilized lactase maintained approximately 95% of catalyst activity. Compared with the free lactase, used as a control, the immobilized lactase was significantly more stable within acidic or basic environments, higher temperature conditions and had multiple recycle use characteristics. Furthermore, the immobilized lactase had a preserved 87% activity after storage at 4 °C for 30 days, while the free lactase was almost deactivated after 20 days under the same storage conditions. This work conforms that the amino-functionalized graphene oxide is a potential support material for lactase immobilization and can be extended to use with a variety of enzymes for a number of applications.

Polymer Functionalized Graphene Oxide: A Versatile Nanoplatform for Drug/Gene Delivery

2015 ◽  
Vol 19 (18) ◽  
pp. 1828-1837 ◽  
Author(s):  
George V. Theodosopoulos ◽  
Panayiotis Bilalis ◽  
Georgios Sakellariou
Keyword(s):  
Graphene Oxide ◽  
Gene Delivery ◽  
Functionalized Graphene ◽  
Functionalized Graphene Oxide

A covalently cross-linked reduced functionalized graphene oxide/polyurethane composite based on Diels–Alder chemistry and its potential application in healable flexible electronics

2017 ◽  
Vol 5 (1) ◽  
pp. 220-228 ◽  
Author(s):  
Jinhui Li ◽  
Guoping Zhang ◽  
Rong Sun ◽  
Ching-Ping Wong
Keyword(s):  
Graphene Oxide ◽  
Flexible Electronics ◽  
Potential Application ◽  
High Efficiency ◽  
Diels Alder ◽  
Functionalized Graphene ◽  
Functionalized Graphene Oxide ◽  
Polyurethane Composite

A novel composite of reduced functionalized graphene oxide/polyurethane based on Diels–Alder chemistry was developed which could be healed microwaves with high efficiency and applied in healable flexible electronics.

Layer by layer assembled functionalized graphene oxide-based polymer brushes for superlubricity on steel-steel tribocontact

Soft Matter ◽  
2021 ◽  
Author(s):  
Suprakash Samanta ◽  
Rashmi Ranjan Sahoo
Keyword(s):  
Graphene Oxide ◽  
Nucleophilic Addition ◽  
Polymer Brushes ◽  
Addition Reaction ◽  
Layer By Layer ◽  
Functionalized Graphene ◽  
Covalent Functionalization ◽  
Functionalized Graphene Oxide ◽  
Nucleophilic Addition Reaction ◽  
Branched Polyethylenimine

Present study demonstrates a simple and multistep approach for the preparation of covalent functionalization of chemically prepared graphene oxide (GO) by branched polyethylenimine (PEI) through nucleophilic addition reaction to prepare...

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