Targeting grainyhead-like transcription factor 2/Snail2 with chitosan-lipid nanoparticles inhibits melanoma progression

2021 ◽  
Vol 11 (7) ◽  
pp. 1192-1199
Author(s):  
Tong Yang ◽  
Lei Jin
Keyword(s):  
Transcription Factor ◽  
Malignant Tumors ◽  
Lipid Nanoparticles ◽  
Cell Proliferation And Migration ◽  
Melanoma Cell Proliferation ◽  
Melanoma Treatment ◽  
And Migration ◽  
And Function ◽  
Melanoma Tissue ◽  
Proliferation And Migration

The grainyhead-like transcription factor 2 (GRHL2) has been reported to be involved in the progression of several malignant tumors; however, its expression and function are still unclear. This study confirmed that GRHL2 expression increased in melanoma tissue and cell lines. We used a novel chitosan-lipid nanoparticle (CS-LNP) nanomaterial to form CS-LNP/plasmids and CS-LNP/siRNAs to upregulate/downregulate GRHL2. The results indicated that GRHL2 promoted melanoma cell proliferation and migration by upregulating the EMT-related snail2 gene, and GRHL2 function in melanoma cells was partially reversed by snail2 knockdown. GRHL2/snail2 could be a potential target in melanoma treatment.

scholarly journals Zerumbone inhibits melanoma cell proliferation and migration by altering mitochondrial functions

2017 ◽  
Vol 13 (4) ◽  
pp. 2397-2402 ◽  
Author(s):  
Hua Yan ◽  
Ming-Yuan Ren ◽  
Zheng-Xiang Wang ◽  
Shi-Jun Feng ◽  
Si Li ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Melanoma Cell ◽  
Cell Proliferation And Migration ◽  
Mitochondrial Functions ◽  
Melanoma Cell Proliferation ◽  
And Migration ◽  
Proliferation And Migration

scholarly journals Neurotrophins and Their Receptors Stimulate Melanoma Cell Proliferation and Migration

2008 ◽  
Vol 128 (8) ◽  
pp. 2031-2040 ◽  
Author(s):  
Francesca Truzzi ◽  
Alessandra Marconi ◽  
Roberta Lotti ◽  
Katiuscia Dallaglio ◽  
Lars E. French ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Melanoma Cell ◽  
Cell Proliferation And Migration ◽  
Melanoma Cell Proliferation ◽  
And Migration ◽  
Proliferation And Migration

scholarly journals LINC01232 exerts oncogenic activities in pancreatic adenocarcinoma via regulation of TM9SF2

2019 ◽  
Vol 10 (10) ◽  
Author(s):  
Qian Li ◽  
Chengbin Lei ◽  
Changliang Lu ◽  
Jingye Wang ◽  
Min Gao ◽  
...  
Keyword(s):  
Pancreatic Adenocarcinoma ◽  
Transcriptional Activation ◽  
Digestive System ◽  
Malignant Tumors ◽  
Loss Of Function ◽  
Protein Coding ◽  
Cellular Processes ◽  
Cell Proliferation And Migration ◽  
And Migration ◽  
Proliferation And Migration

Abstract Pancreatic adenocarcinoma (PAAD), one of the most prevailing malignant tumors in digestive system, is identified as one of the main culprits of cancer-associated mortality. Despite long intergenic non-protein coding RNA 1232 (LINC01232) is found to be upregulated in TCGA PAAD tissues and associated with poor prognosis, the potential of LINC01232 in PAAD progression still needs more explorations. In this study, LINC01232 was chosen to be the research object in PAAD cellular processes. Functionally, loss-of function assays were carried out and the experimental results indicated that suppression of LINC01232 hindered the deterioration of PAAD by affecting cell proliferation and migration. Furthermore, relationship between LINC01232 and its nearby gene transmembrane 9 superfamily member 2 (TM9SF2) was investigated. The same expression pattern of TM9SF2 in TCGA PAAD samples was observed. It was found that upregulation of LINC01232 could be a crucial factor for the dysregulation of TM9SF2. Mechanistically, LINC01232 recruited EIF4A3 to boost TM9SF2 mRNA stability. Besides, our findings demonstrated that the transcriptional activation of LINC01232 and TM9SF2 was mediated by SP1. Therefore, we concluded that LINC01232 executed carcinogenic properties in PAAD progression via regulation of TM9SF2. In conclusion, this study was the first to unveil the role and molecular mechanism of LINC01232, suggesting LINC01232 as a promising molecular target for pancreatic cancer treatment.

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