Three-Dimensional Non-Volatile Magnetic Universal Logic Gates

2011 ◽  
Vol 6 (2) ◽  
pp. 132-137
Author(s):  
N. Amos ◽  
E. Stefanescu ◽  
J. Butler ◽  
B. Lee ◽  
Y. Tian ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Logic Gates ◽  
Universal Logic

scholarly journals Universal logic gates via liquid-electronic hybrid divider

Lab on a Chip ◽  
2012 ◽  
Vol 12 (24) ◽  
pp. 5211 ◽  
Author(s):  
Bingpu Zhou ◽  
Limu Wang ◽  
Shunbo Li ◽  
Xiang Wang ◽  
Yu Sanna Hui ◽  
...  
Keyword(s):  
Logic Gates ◽  
Universal Logic

scholarly journals Miswired enhancer logic drives translocation positive rhabdomyosarcoma

2019 ◽  
Author(s):  
Berkley E. Gryder ◽  
Marco Wachtel ◽  
Kenneth Chang ◽  
Osama El Demerdash ◽  
Nicholas G. Aboreden ◽  
...  
Keyword(s):  
Muscle Development ◽  
Dimensional Space ◽  
Three Dimensional ◽  
Logic Gates ◽  
Physical Interaction ◽  
Rna Seq ◽  
Super Enhancer ◽  
Infinite Loop ◽  
Genomic Regions ◽  
Three Dimensional Space

AbstractCore regularity transcription factors (CR TFs) define cell identity and lineage through an exquisitely precise and logical order during embryogenesis and development. These CR TFs regulate one another in three-dimensional space via distal enhancers that serve as logic gates embedded in their TF recognition sequences. Aberrant chromatin organization resulting in miswired circuitry of enhancer logic is a newly recognized feature in many cancers. Here, we report that PAX3-FOXO1 expression is driven by a translocated FOXO1 distal super enhancer (SE). Using 4C-seq, a technique detecting all genomic regions that interact with the translocated FOXO1 SE, we demonstrate its physical interaction with the PAX3 promotor only in the presence of the oncogenic translocation. Furthermore, RNA-seq and ChIP-seq in tumors bearing rare PAX translocations implicate enhancer miswiring is a pervasive feature across all FP-RMS tumors. HiChIP of enhancer mark H3K27ac showed extended connectivity between the distal FOXO1 SE and additional intra-domain enhancers and the PAX3 promoter. We show by CRISPR-paired-ChIP-Rx that PAX3-FOXO1 transcription is diminished when this network of enhancers is selectively ablated. Therefore, our data reveal a mechanism of a translocated hijacked enhancer which disrupts the normal CR TF logic during skeletal muscle development (PAX3 to MYOD to MYOG), replacing it with an infinite loop logic that locks rhabdomyosarcoma cells in an undifferentiated proliferating stage.

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