A Novel Unmodified Gold Nanoparticles-Based Assay for Direct Detection of Unamplified Bovine Viral Diarrhea Virus-RNA

2016 ◽  
Vol 16 (12) ◽  
pp. 12344-12350 ◽  
Author(s):  
Zahra Heidari ◽  
Seyedeh Elham Rezatofighi ◽  
Saadat Rastegarzadeh
Keyword(s):  
Gold Nanoparticles ◽  
Bovine Viral Diarrhea Virus ◽  
Direct Detection ◽  
Bovine Viral Diarrhea ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Virus Rna ◽  
Viral Diarrhea Virus

scholarly journals Development and Comparison of Cross-Linking and Non-Crosslinking Probe-Gold Nanoparticle Hybridization Assays for Direct Detection of Unamplified Bovine Viral Diarrhea Virus-RNA

2020 ◽  
Author(s):  
Zahra Heidari ◽  
Seyedeh Elham Rezatofighi ◽  
Saadat Rastegarzadeh
Keyword(s):  
Bovine Viral Diarrhea Virus ◽  
Color Change ◽  
Direct Detection ◽  
Response Speed ◽  
Cross Linking ◽  
Bovine Viral Diarrhea ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Viral Diarrhea Virus ◽  
Induced Aggregation

Abstract Background: Bovine viral diarrhea virus (BVDV) is a major economic disease that has been spread in most countries. In addition to vaccination, one of the main ways to control the disease and prevent it from spreading is to detect and cull infected animals, especially those with persistent infection (PI). We developed and compared two colorimetric biosensor assays based on probe-modified gold nanoparticles (AuNPs) to detect BVDV. Specific probes were designed to detect the 5ʹ untranslated region of BVDV-RNA. The thiolated probes were immobilized on the surface of the AuNPs. Two methods of cross-linking (CL) and non-crosslinking (NCL) probe-AuNPs hybridization were developed and compared. Results: The hybridization of positive targets with the two probe-AuNPs formed a polymeric network between the AuNPs which led to the aggregation of nanoparticles and color change from red to blue. Alternatively, in the NCL mode, the hybridization of complementary targets with the probe-AuNPs resulted in the increased electrostatic repulsion in nanoparticles and the increased stabilization against salt-induced aggregation. The CL and NCL assays had detection limits of 6.83 and 44.36 ng/reaction, respectively. Conclusion: The CL assay showed a higher sensitivity and specificity; in contrast, the NCL assay did not require optimizing and controlling of hybridization temperature and showed a higher response speed. However, both the developed methods are cost-effective and easy to perform and also could be implemented on-site or in local laboratories in low-resource countries.

scholarly journals Inhibition of Bovine Viral Diarrhea Virus RNA Synthesis by Thiosemicarbazone Derived from 5,6-Dimethoxy-1-Indanone

2011 ◽  
Vol 85 (11) ◽  
pp. 5436-5445 ◽  
Author(s):  
E. F. Castro ◽  
L. E. Fabian ◽  
M. E. Caputto ◽  
D. Gagey ◽  
L. M. Finkielsztein ◽  
...  
Keyword(s):  
Bovine Viral Diarrhea Virus ◽  
Rna Synthesis ◽  
Bovine Viral Diarrhea ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Virus Rna ◽  
Viral Diarrhea Virus

scholarly journals Development and Comparison of Cross-Linking and Non-Crosslinking Probe-Gold Nanoparticle Hybridization Assays for Direct Detection of Unamplified Bovine Viral Diarrhea Virus-RNA

2021 ◽  
Author(s):  
Zahra Heidari ◽  
Seyedeh Elham Rezatofighi ◽  
Saadat Rastegarzadeh
Keyword(s):  
Bovine Viral Diarrhea Virus ◽  
Color Change ◽  
Direct Detection ◽  
Response Speed ◽  
Cross Linking ◽  
Bovine Viral Diarrhea ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Viral Diarrhea Virus ◽  
Induced Aggregation

Abstract Background: Bovine viral diarrhea virus (BVDV) is a major economic disease that has been spread in most countries. In addition to vaccination, one of the main ways to control the disease and prevent it from spreading is to detect and cull infected animals, especially those with persistent infection (PI). We developed and compared two colorimetric biosensor assays based on probe-modified gold nanoparticles (AuNPs) to detect BVDV. Specific probes were designed to detect the 5ʹ untranslated region of BVDV-RNA. The thiolated probes were immobilized on the surface of the AuNPs. Two methods of cross-linking (CL) and non-crosslinking (NCL) probe-AuNPs hybridization were developed and compared. Results: The hybridization of positive targets with the two probe-AuNPs formed a polymeric network between the AuNPs which led to the aggregation of nanoparticles and color change from red to blue. Alternatively, in the NCL mode, the hybridization of complementary targets with the probe-AuNPs resulted in the increased electrostatic repulsion in nanoparticles and the increased stabilization against salt-induced aggregation. The CL and NCL assays had detection limits of 6.83 and 44.36 ng/reaction, respectively. Conclusion: The CL assay showed a higher sensitivity and specificity; in contrast, the NCL assay did not require optimizing and controlling of hybridization temperature and showed a higher response speed. However, both the developed methods are cost-effective and easy to perform and also could be implemented on-site or in local laboratories in low-resource countries.

scholarly journals Development and comparison of cross-linking and non-crosslinking probe-gold nanoparticle hybridization assays for direct detection of unamplified bovine viral diarrhea virus-RNA

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Zahra Heidari ◽  
Seyedeh Elham Rezatofighi ◽  
Saadat Rastegarzadeh
Keyword(s):  
Bovine Viral Diarrhea Virus ◽  
Color Change ◽  
Direct Detection ◽  
Response Speed ◽  
Cross Linking ◽  
Bovine Viral Diarrhea ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Viral Diarrhea Virus ◽  
Induced Aggregation

Abstract Background Bovine viral diarrhea virus (BVDV) is a major economic disease that has been spread in most countries. In addition to vaccination, one of the main ways to control the disease and prevent it from spreading is to detect and cull infected animals, especially those with persistent infection (PI). We developed and compared two colorimetric biosensor assays based on probe-modified gold nanoparticles (AuNPs) to detect BVDV. Specific probes were designed to detect the 5′ untranslated region of BVDV-RNA. The thiolated probes were immobilized on the surface of the AuNPs. Two methods of cross-linking (CL) and non-crosslinking (NCL) probe-AuNPs hybridization were developed and compared. Results The hybridization of positive targets with the two probe-AuNPs formed a polymeric network between the AuNPs which led to the aggregation of nanoparticles and color change from red to blue. Alternatively, in the NCL mode, the hybridization of complementary targets with the probe-AuNPs resulted in the increased electrostatic repulsion in nanoparticles and the increased stabilization against salt-induced aggregation. The CL and NCL assays had detection limits of 6.83 and 44.36 ng/reaction, respectively. Conclusion The CL assay showed a higher sensitivity and specificity; in contrast, the NCL assay did not require optimizing and controlling of hybridization temperature and showed a higher response speed. However, both the developed methods are cost-effective and easy to perform and also could be implemented on-site or in local laboratories in low-resource countries.

Different Molecular Mechanisms of Inhibition of Bovine Viral Diarrhea Virus and Hepatitis C Virus RNA-Dependent RNA Polymerases by a Novel Benzimidazole

Biochemistry ◽  
2013 ◽  
Vol 52 (21) ◽  
pp. 3752-3764 ◽  
Author(s):  
Shailendra Asthana ◽  
Saumya Shukla ◽  
Attilio V. Vargiu ◽  
Matteo Ceccarelli ◽  
Paolo Ruggerone ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Bovine Viral Diarrhea Virus ◽  
Molecular Mechanisms ◽  
Bovine Viral Diarrhea ◽  
Hepatitis C Virus Rna ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Virus Rna ◽  
Viral Diarrhea Virus
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