Identification of Surface-Enhanced Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry as Predictors of Prognosis in Triple Negative Breast Cancer

2016 ◽  
Vol 16 (12) ◽  
pp. 12483-12488
Author(s):  
Ming Fan ◽  
Qingbang Yu ◽  
Xiaojia Wang ◽  
Zhiguo Zheng ◽  
Shenhua Xu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Mass Spectrometry ◽  
Triple Negative Breast Cancer ◽  
Triple Negative ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Surface Enhanced

Detection of Breast Cancer by Surface-enhanced Laser Desorption/Ionization Time-of-flight Mass Spectrometry Tissue and Serum Protein Profiling

2009 ◽  
Vol 24 (3) ◽  
pp. 130-141 ◽  
Author(s):  
Marie-Christine W. Gast ◽  
Eric J. van Dulken ◽  
Thea K.G. van Loenen ◽  
Florine Kingma-Vegter ◽  
Johan Westerga ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Mass Spectrometry ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Tissue Samples ◽  
Ionization Time ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Surface Enhanced ◽  
Tof Ms

Aim Novel diagnostic breast cancer markers have been extensively searched for in the proteome, using, among others, surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS). Thus far, the majority of SELDI-TOF MS studies have investigated samples originating from biorepositories, which hampers biomarker discovery as they likely suffer from variable adherence to collection protocols. Material and methods We investigated breast cancer (n=75) and control (n=26) serum and tissue samples, collected prospectively by rigorous adherence to a strictly defined protocol. Sera were collected preoperatively and postoperatively, and serum and tissue samples were analyzed by SELDI-TOF MS using the IMAC30 Ni and Q10 pH 8 array. Results Three serum peaks were significantly associated with breast cancer, while in tissue, 27 discriminative peaks were detected. Several peak clusters gradually increased or decreased in intensity from healthy to benign to cancer, or with increasing cancer stage. The constructed classification trees had a tenfold cross-validated performance of 67% to 87%. Two tissue peaks were identified as N-terminal albumin fragments. These are likely to have been generated by (breast) cancer-specific proteolytic activity in the tumor microenvironment. Conclusions These albumin fragments can potentially provide insights into the pathophysiological mechanisms associated with, or underlying, breast cancer, and aid in improving breast cancer diagnosis.

Investigation of Tissue Inhibitor of Metalloproteinases 1 in Plasma from Colorectal Cancer Patients and Blood Donors by Surface-Enhanced Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

2007 ◽  
Vol 22 (2) ◽  
pp. 89-94 ◽  
Author(s):  
M.B. Caspersen ◽  
N.M. Sørensen ◽  
A.S. Schrohl ◽  
P. Iversen ◽  
H.J. Nielsen ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Mass Spectrometry ◽  
Laser Desorption ◽  
Tissue Inhibitor Of Metalloproteinases ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Surface Enhanced ◽  
Tof Ms

Early detection of colorectal cancer (CRC) improves patient survival. Plasma tissue inhibitor of metalloproteinases 1 (TIMP-1) measurements by enzyme-linked immunosorbent assay (ELISA) have been suggested as a new method for the early detection of CRC. To further investigate the nature of TIMP-1 in plasma, surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI TOF MS) was used. TIMP-1 measurements of plasma from 16 healthy donors and 14 CRC patients were performed using TIMP-1 monoclonal antibody in SELDI TOF MS and ELISA. SELDI TOF MS applying an antibody to TIMP-1 revealed that human plasma TIMP-1 has a mass of 25.1 kDa and exhibits several isoforms. Both methods showed increased plasma TIMP-1 values for cancer patients as compared to healthy individuals. The p values for the separation of the groups were 0.0019 for ELISA and <0.0001 for SELDI TOF MS. CRC did not fundamentally affect the appearance of TIMP-1 as evaluated by SELDI TOF MS.

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