In Vitro Evaluation on a Model of Blood Brain Barrier of Idebenone-Loaded Solid Lipid Nanoparticles

2012 ◽  
Vol 12 (1) ◽  
pp. 330-337 ◽  
Author(s):  
L. Montenegro ◽  
A. Trapani ◽  
A. Latrofa ◽  
G. Puglisi
Keyword(s):  
Blood Brain Barrier ◽  
Solid Lipid Nanoparticles ◽  
Lipid Nanoparticles ◽  
Brain Barrier ◽  
Solid Lipid ◽  
Blood Brain

Solid lipid nanoparticles for delivery of andrographolide across the blood-brain barrier: in vitro and in vivo evaluation

2018 ◽  
Vol 161 ◽  
pp. 302-313 ◽  
Author(s):  
Giulia Graverini ◽  
Vieri Piazzini ◽  
Elisa Landucci ◽  
Daniela Pantano ◽  
Pamela Nardiello ◽  
...  
Keyword(s):  
Blood Brain Barrier ◽  
Solid Lipid Nanoparticles ◽  
Lipid Nanoparticles ◽  
Brain Barrier ◽  
In Vivo Evaluation ◽  
Solid Lipid ◽  
Blood Brain

scholarly journals Methotrexate-Loaded Solid Lipid Nanoparticles: Protein Functionalization to Improve Brain Biodistribution

Pharmaceutics ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 65 ◽  
Author(s):  
Elisabetta Muntoni ◽  
Katia Martina ◽  
Elisabetta Marini ◽  
Marta Giorgis ◽  
Loretta Lazzarato ◽  
...  
Keyword(s):  
Blood Brain Barrier ◽  
Solid Lipid Nanoparticles ◽  
Lipid Nanoparticles ◽  
Brain Barrier ◽  
Target Tissue ◽  
Solid Lipid ◽  
Blood Brain ◽  
The Central Nervous System

Glioblastoma is the most common and invasive primary tumor of the central nervous system and normally has a negative prognosis. Biodistribution in healthy animal models is an important preliminary study aimed at investigating the efficacy of chemotherapy, as it is mainly addressed towards residual cells after surgery in a region with an intact blood–brain barrier. Nanoparticles have emerged as versatile vectors that can overcome the blood–brain barrier. In this experimental work, solid lipid nanoparticles, prepared using fatty acid coacervation, have been loaded with an active lipophilic ester of cytotoxic drug methotrexate, and functionalized with either transferrin or insulin, two proteins whose receptors are abundantly expressed on the blood–brain barrier. Functionalization has been achieved by grafting a maleimide moiety onto the nanoparticle’s surface and exploiting its reactivity towards thiolated proteins. The nanoparticles have been tested in vitro on a blood–brain barrier cellular model and in vivo for biodistribution in Wistar rats. Drug metabolites, in particular 7-hydroxymethotrexate, have also been investigated in the animal model. The data obtained indicate that the functionalization of the nanoparticles improved their ability to overcome the blood–brain barrier when a PEG spacer between the proteins and the nanoparticle’s surface was used. This is probably because this method provided improved ligand–receptor interactions and selectivity for the target tissue.

scholarly journals ApoE-Targeting Increases the Transfer of Solid Lipid Nanoparticles with Donepezil Cargo across a Culture Model of the Blood–Brain Barrier

Pharmaceutics ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 38
Author(s):  
Gizem Rüya Topal ◽  
Mária Mészáros ◽  
Gergő Porkoláb ◽  
Anikó Szecskó ◽  
Tamás Ferenc Polgár ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Blood Brain Barrier ◽  
Solid Lipid Nanoparticles ◽  
Lipid Nanoparticles ◽  
Brain Barrier ◽  
Brain Targeting ◽  
Solid Lipid ◽  
Blood Brain ◽  
Culture Model ◽  
The Brain

Pharmacological treatment of central nervous system (CNS) disorders is difficult, because the blood–brain barrier (BBB) restricts the penetration of many drugs into the brain. To solve this unmet therapeutic need, nanosized drug carriers are the focus of research efforts to develop drug delivery systems for the CNS. For the successful delivery of nanoparticles (NPs) to the brain, targeting ligands on their surface is necessary. Our research aim was to design a nanoscale drug delivery system for a more efficient transfer of donepezil, an anticholinergic drug in the therapy of Alzheimer’s disease across the BBB. Rhodamine B-labeled solid lipid nanoparticles with donepezil cargo were prepared and targeted with apolipoprotein E (ApoE), a ligand of BBB receptors. Nanoparticles were characterized by measurement of size, polydispersity index, zeta potential, thermal analysis, Fourier-transform infrared spectroscopy, in vitro release, and stability. Cytotoxicity of nanoparticles were investigated by metabolic assay and impedance-based cell analysis. ApoE-targeting increased the uptake of lipid nanoparticles in cultured brain endothelial cells and neurons. Furthermore, the permeability of ApoE-targeted nanoparticles across a co-culture model of the BBB was also elevated. Our data indicate that ApoE, which binds BBB receptors, can potentially be exploited for successful CNS targeting of solid lipid nanoparticles.

ApoE-modified solid lipid nanoparticles: A feasible strategy to cross the blood-brain barrier

2017 ◽  
Vol 249 ◽  
pp. 103-110 ◽  
Author(s):  
R. Dal Magro ◽  
F. Ornaghi ◽  
I. Cambianica ◽  
S. Beretta ◽  
F. Re ◽  
...  
Keyword(s):  
Blood Brain Barrier ◽  
Solid Lipid Nanoparticles ◽  
Lipid Nanoparticles ◽  
Brain Barrier ◽  
Solid Lipid ◽  
Blood Brain

scholarly journals Solid Lipid Nanoparticles for Brain Targeting

2019 ◽  
Vol 9 (6-s) ◽  
pp. 248-252
Author(s):  
Sachin Raosaheb Hangargekar ◽  
Pradeepkumar Mohanty ◽  
Ashish Jain
Keyword(s):  
Drug Delivery ◽  
Blood Brain Barrier ◽  
Solid Lipid Nanoparticles ◽  
Drug Loading ◽  
Specific Area ◽  
Lipid Nanoparticles ◽  
Brain Barrier ◽  
Brain Targeting ◽  
Solid Lipid ◽  
Blood Brain

Brain is considered to be highly impermeable barrier, possessing different obstacles like presence of enzymes, presence of tight junctions that limit the entry for most of the drugs. The presence of these obstacles, possess a challenge for administration of the drugs. The conventional means of drug delivery in form of emulsions, fail to overcome these obstacles, and hence there is a need for newer drug delivery approach, that will cross these barriers of the brain. So, these nanoparticles can be an alternative to other conventional systems. They offer several advantages such as improved bioavailability and solubility that are composed of macromolecular materials like lipids and polymers possess low cytotoxicity, high drug loading capability, and good scalability these are the most effective colloidal carriers that have the ability to incorporate drugs into nanocarriers and used as drug targeting to specific area. Thus, this article will emphasise on properties of Blood Brain Barrier, strategies to overcome the blood–brain barrier, literature regarding the use of SLNs in various neurological disease states, production methods of SLN and its evaluation. Hence, these solid lipid formulations can be a new form and one of the promising approach for drug delivery system in future, that have remarkable possibility to cross the BBB. Keywords: Solid lipid nanoparticles, Nanocarriers, Blood–brain barrier

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