In-Situ Detection of C-Reactive Protein Using Silicon Nanowire Field Effect Transistor

2011 ◽  
Vol 11 (2) ◽  
pp. 1511-1514 ◽  
Author(s):  
Soon Mook Kwon ◽  
Gil Bum Kang ◽  
Yong Tae Kim ◽  
Young-Hwan Kim ◽  
Byeong-Kwon Ju
Keyword(s):  
Field Effect ◽  
Field Effect Transistor ◽  
Silicon Nanowire ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Effect Transistor ◽  
In Situ Detection

scholarly journals Sensing of C-Reactive Protein Using an Extended-Gate Field-Effect Transistor with a Tungsten Disulfide-Doped Peptide-Imprinted Conductive Polymer Coating

Biosensors ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 31
Author(s):  
Kai-Hsi Liu ◽  
Hung-Yin Lin ◽  
James L. Thomas ◽  
Chen-Yuan Chen ◽  
Yen-Ting Chen ◽  
...  
Keyword(s):  
Field Effect ◽  
Field Effect Transistor ◽  
Inflammatory Responses ◽  
Polymeric Materials ◽  
Tungsten Disulfide ◽  
Electrochemical Sensing ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Chip Technology ◽  
Effect Transistor

C-reactive protein (CRP) is a non-specific biomarker of inflammation and may be associated with cardiovascular disease. In recent studies, systemic inflammatory responses have also been observed in cases of coronavirus disease 2019 (COVID-19). Molecularly imprinted polymers (MIPs) have been developed to replace natural antibodies with polymeric materials that have low cost and high stability and could thus be suitable for use in a home-care system. In this work, a MIP-based electrochemical sensing system for measuring CRP was developed. Such a system can be integrated with microfluidics and electronics for lab-on-a-chip technology. MIP composition was optimized using various imprinting template (CRP peptide) concentrations. Tungsten disulfide (WS2) was doped into the MIPs. Doping not only enhances the electrochemical response accompanying the recognition of the template molecules but also raises the top of the sensing range from 1.0 pg/mL to 1.0 ng/mL of the imprinted peptide. The calibration curve of the WS2-doped peptide-imprinted polymer-coated electrodes in the extended-gate field-effect transistor platform was obtained and used for the measurement of CRP concentration in real human serum.

scholarly journals Silicon Nanowire Field-Effect Transistor as Next Generation Biochip for Post-Translational Modification

2020 ◽  
Author(s):  
Ping-Chia Su ◽  
Bo-Han Chen ◽  
Yi-Chan Lee ◽  
Yuh-Shyong Yang
Keyword(s):  
Protein Interactions ◽  
Field Effect ◽  
Field Effect Transistor ◽  
Silicon Nanowire ◽  
Drain Current ◽  
Next Generation ◽  
Post Translational Modification ◽  
Tyrosine Sulfation ◽  
Effect Transistor

Protein tyrosine sulfation (PTS), a vital post-translational modification, facilitates protein–protein interactions and regulates many physiological and pathological responses. Monitoring PTS has been difficult owing to the instability of sulfated proteins and the lack of a suitable method for detecting the protein sulfate ester. In this study, we combined an in situ PTS system with an ultra-high-sensitivity polysilicon nanowire field-effect transistor (pSNWFET)-based sensor to directly monitor PTS formation. A peptide containing the tyrosine sulfation site of P-selectin glycoprotein ligand (PSGL)-1 was immobilized onto the surface of the pSNWFET by using 3-aminopropyltriethoxysilane and glutaraldehyde as linker molecules. A coupled enzyme sulfation system consisting of tyrosylprotein sulfotransferase and phenol sulfotransferase was used to catalyze PTS of the immobilized PSGL-1 peptide. Enzyme-catalyzed sulfation of the immobilized peptide was readily observed through the shift of the drain current–gate voltage curves of the pSNWFET before and after PTS. To the best of our knowledge, this is the first study to describe in situ PTS and its direct observation by using semiconductor devices. We expect that this approach can be developed as a next generation biochip for biomedical research and industries.

In Situ Detection of Chromogranin A Released from Living Neurons with a Single-Walled Carbon-Nanotube Field-Effect Transistor

Small ◽  
2007 ◽  
Vol 3 (8) ◽  
pp. 1350-1355 ◽  
Author(s):  
Chen-Wei Wang ◽  
Chien-Yuan Pan ◽  
Hsing-Chen Wu ◽  
Po-Yuan Shih ◽  
Chia-Chang Tsai ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Chromogranin A ◽  
Field Effect ◽  
Field Effect Transistor ◽  
Single Walled Carbon Nanotube ◽  
Single Walled Carbon ◽  
Effect Transistor ◽  
Living Neurons ◽  
In Situ Detection

Label-free C-reactive protein electronic detection with an electrolyte-gated organic field-effect transistor-based immunosensor

2016 ◽  
Vol 408 (15) ◽  
pp. 3943-3952 ◽  
Author(s):  
Maria Magliulo ◽  
Donato De Tullio ◽  
Inger Vikholm-Lundin ◽  
Willem M. Albers ◽  
Tony Munter ◽  
...  
Keyword(s):  
Field Effect ◽  
Field Effect Transistor ◽  
Label Free ◽  
C Reactive Protein ◽  
Organic Field Effect Transistor ◽  
Reactive Protein ◽  
Electronic Detection ◽  
Effect Transistor

Monitoring of C-Reactive Protein Using Ion Sensitive Field Effect Transistor Biosensor

2010 ◽  
Vol 8 (2) ◽  
pp. 233-237 ◽  
Author(s):  
Hye-Jung Park ◽  
Sang Kyu Kim ◽  
Kyoungsook Park ◽  
So Yeon Yi ◽  
Jin Woong Chung ◽  
...  
Keyword(s):  
Field Effect ◽  
Field Effect Transistor ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Effect Transistor
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