Cell Uptake and Intracellular Visualization Using Quantum Dots or Nuclear Localization Signal-Modified Quantum Dots with Gold Nanoparticles as Quenchers

2010 ◽  
Vol 10 (7) ◽  
pp. 4173-4177 ◽  
Author(s):  
Kai-Wei Kuo ◽  
Te-Hsuen Chen ◽  
Wei-Ti Kuo ◽  
Hong-Yi Huang ◽  
Hsien-Yi Lo ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Gold Nanoparticles ◽  
Nuclear Localization ◽  
Nuclear Localization Signal ◽  
Cell Uptake ◽  
Localization Signal

Cell Uptake and Radiotoxicity Studies of an Nuclear Localization Signal Peptide−Intercalator Conjugate Labeled with [99mTc(CO)3]+

2005 ◽  
Vol 16 (3) ◽  
pp. 582-587 ◽  
Author(s):  
Pascal Haefliger ◽  
Nikos Agorastos ◽  
Annabelle Renard ◽  
Guya Giambonini-Brugnoli ◽  
Cornelia Marty ◽  
...  
Keyword(s):  
Nuclear Localization ◽  
Signal Peptide ◽  
Nuclear Localization Signal ◽  
Cell Uptake ◽  
Localization Signal

scholarly journals Nuclear-targeted siRNA delivery for long-term gene silencing

2017 ◽  
Vol 8 (4) ◽  
pp. 2816-2822 ◽  
Author(s):  
Na Li ◽  
Huijun Yang ◽  
Zhengze Yu ◽  
Yanli Li ◽  
Wei Pan ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Gene Silencing ◽  
Cancer Cells ◽  
Nuclear Localization ◽  
Nuclear Localization Signal ◽  
Sirna Delivery ◽  
Signal Peptides ◽  
Localization Signal ◽  
Sirna Delivery System

A nuclear-targeted siRNA delivery system was developed for long-term gene silencing in cancer cells. The nanocarrier consists of gold nanoparticles, synthetic siRNAs and nuclear localization signal peptides.

scholarly journals Coordinate Nuclear Targeting of the FANCD2 and FANCI Proteins via a FANCD2 Nuclear Localization Signal

PLoS ONE ◽  
2013 ◽  
Vol 8 (11) ◽  
pp. e81387 ◽  
Author(s):  
Rebecca A. Boisvert ◽  
Meghan A. Rego ◽  
Paul A. Azzinaro ◽  
Maurizio Mauro ◽  
Niall G. Howlett
Keyword(s):  
Nuclear Localization ◽  
Nuclear Localization Signal ◽  
Nuclear Targeting ◽  
Localization Signal

scholarly journals SUMO-1 Modification and Its Role in Targeting the Ran GTPase-activating Protein, RanGAP1, to the Nuclear Pore Complex

1998 ◽  
Vol 140 (3) ◽  
pp. 499-509 ◽  
Author(s):  
Michael J. Matunis ◽  
Jian Wu ◽  
Günter Blobel
Keyword(s):  
Nuclear Localization ◽  
Nuclear Localization Signal ◽  
Nuclear Export ◽  
Nuclear Export Signal ◽  
Nucleocytoplasmic Transport ◽  
Nuclear Pore ◽  
Nuclear Pore Complexes ◽  
Gtpase Activating Protein ◽  
Terminal Domain ◽  
Localization Signal

RanGAP1 is the GTPase-activating protein for Ran, a small ras-like GTPase involved in regulating nucleocytoplasmic transport. In vertebrates, RanGAP1 is present in two forms: one that is cytoplasmic, and another that is concentrated at the cytoplasmic fibers of nuclear pore complexes (NPCs). The NPC-associated form of RanGAP1 is covalently modified by the small ubiquitin-like protein, SUMO-1, and we have recently proposed that SUMO-1 modification functions to target RanGAP1 to the NPC. Here, we identify the domain of RanGAP1 that specifies SUMO-1 modification and demonstrate that mutations in this domain that inhibit modification also inhibit targeting to the NPC. Targeting of a heterologous protein to the NPC depended on determinants specifying SUMO-1 modification and also on additional determinants in the COOH-terminal domain of RanGAP1. SUMO-1 modification and these additional determinants were found to specify interaction between the COOH-terminal domain of RanGAP1 and a region of the nucleoporin, Nup358, between Ran-binding domains three and four. Together, these findings indicate that SUMO-1 modification targets RanGAP1 to the NPC by exposing, or creating, a Nup358 binding site in the COOH-terminal domain of RanGAP1. Surprisingly, the COOH-terminal domain of RanGAP1 was also found to harbor a nuclear localization signal. This nuclear localization signal, and the presence of nine leucine-rich nuclear export signal motifs, suggests that RanGAP1 may shuttle between the nucleus and the cytoplasm.

scholarly journals Definition of a major p53 binding site on Ad2E1B58K protein and a possible nuclear localization signal on the Ad12E1B54K protein

Oncogene ◽  
1999 ◽  
Vol 18 (4) ◽  
pp. 955-965 ◽  
Author(s):  
Roger JA Grand ◽  
Julian Parkhill ◽  
Tadge Szestak ◽  
Susan M Rookes ◽  
Sally Roberts ◽  
...  
Keyword(s):  
Binding Site ◽  
Nuclear Localization ◽  
Nuclear Localization Signal ◽  
Localization Signal ◽  
Definition Of
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