Study on the Binding of Procaine Hydrochloride to DNA/DNA Bases and the Effect of CdS Nanoparticles on the Binding Behavior

2006 ◽  
Vol 6 (1) ◽  
pp. 91-94 ◽  
Author(s):  
Gang Ping ◽  
Gang Lv ◽  
Sebastian Gutmann ◽  
Chen Chen ◽  
Renyun Zhang ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Differential Pulse Voltammetry ◽  
Differential Pulse ◽  
Dna Bases ◽  
Procaine Hydrochloride ◽  
Cds Nanoparticles ◽  
Force Microscopy ◽  
Binding Behavior ◽  
Atomic Force ◽  
Pulse Voltammetry

The interaction between procaine hydrochloride and DNA/DNA bases in the absence and presence of cadmium sulfide (CdS) nanoparticles has been explored in this study by using differential pulse voltammetry, atomic force microscopy (AFM) and so on, which illustrates the different binding behaviors of procaine hydrochloride with different DNA bases. The results clearly indicate that the binding of purines to procaine hydrochloride is stronger than that of pyrimidines and the binding affinity is in the order of G > A > T > C. In addition, it was observed that the presence of CdS nanoparticles could remarkably enhance the probing sensitivity for the interaction between procaine hydrochloride and DNA/DNA bases. Furthermore, AFM study illustrates that procaine hydrochloride can bind to some specific sites of DNA chains, which indicates that procaine hydrochloride may interact with some special sequences of DNA.

Imaging and quantifying analysis the binding behavior of PD-L1 at molecular resolution by atomic force microscopy

2021 ◽  
pp. 339281
Author(s):  
Juan Qin ◽  
Miaomiao Zhang ◽  
Yanxue Guan ◽  
Xinyue Guo ◽  
Zongjia Li ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Force Microscopy ◽  
Binding Behavior ◽  
Atomic Force

CeO2 thin film for mediator-less glucose biosensors

2008 ◽  
Vol 1138 ◽  
Author(s):  
Shibu Saha ◽  
S. K. Arya ◽  
S. P. Singh ◽  
B. D. Malhotra ◽  
K. Sreenivas ◽  
...  
Keyword(s):  
Thin Film ◽  
Atomic Force Microscopy ◽  
Pulsed Laser ◽  
Differential Pulse ◽  
Optical Measurements ◽  
Glucose Biosensors ◽  
Force Microscopy ◽  
Atomic Force ◽  
Nanoporous Surface ◽  
Menten Constant

AbstractPulsed laser deposited cerium oxide (CeO2) nanoporous thin film on platinum (Pt) coated glass has been used for immobilization of glucose oxidase (GOx) by electrostatic interaction. Atomic force microscopy studies reveal the formation of nanoporous surface morphology of CeO2 thin film. Differential pulse voltammetric and optical measurements show that the GOx/CeO2/Pt bioelectrode is sensitive to the detection of glucose over the concentration upto 300 mg/dl. A low value of enzyme's kinetic parameter (Michaelis-Menten constant∼1.01 mM) indicates enhanced enzyme affinity of GOx to glucose.

scholarly journals Application of a Conducting Poly-Methionine/Gold Nanoparticles-Modified Sensor for the Electrochemical Detection of Paroxetine

Polymers ◽  
2021 ◽  
Vol 13 (22) ◽  
pp. 3981
Author(s):  
Saedah R. Al-Mhyawi ◽  
Riham K. Ahmed ◽  
Rasha M. El Nashar
Keyword(s):  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Electrochemical Techniques ◽  
Pharmaceutical Formulations ◽  
Differential Pulse ◽  
Force Microscopy ◽  
Atomic Force ◽  
Response Range ◽  
Pulse Voltammetry ◽  
Modified Sensor

This work demonstrates a facile electropolymerization of a dl-methionine (dl-met) conducting polymeric film on a gold nanoparticle (AuNPs)-modified glassy carbon electrode (GCE). The resulting sensor was successfully applied for the sensitive detection of paroxetine·HCl (PRX), a selective serotonin (5-HT) reuptake inhibitor (SSRIs), in its pharmaceutical formulations. The sensor was characterized morphologically using scanning electron microscopy (SEM) with energy dispersive X-ray spectroscopy (EDX) and atomic force microscopy (AFM) and electrochemical techniques such as differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The proposed sensor, poly (dl-met)/AuNPs-GCE, exhibited a linear response range from 5 × 10−11 to 5 × 10−8 M and from 5 × 10−8 to 1 × 10−4 M using DPV with lowest limit of detection (LOD = 1 × 10−11 M) based on (S/N = 3). The poly (dl-met)/AuNPs-GCE sensor was successfully applied for PRX determination in three different pharmaceutical formulations with percent recoveries between 96.29% and 103.40% ± SD (±0.02 and ±0.58, respectively).

Electrostatic Forces on the Surface of Metals as Measured by Atomic Force Microscopy

2000 ◽  
Vol 10 (1-2) ◽  
pp. 15
Author(s):  
Eugene Sprague ◽  
Julio C. Palmaz ◽  
Cristina Simon ◽  
Aaron Watson
Keyword(s):  
Atomic Force Microscopy ◽  
Electrostatic Forces ◽  
Force Microscopy ◽  
Atomic Force
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