Separation of Tissue Proteins of Human Lung Carcinomas by Partial-Filling Capillary Electrophoresis

2005 ◽  
Vol 5 (8) ◽  
pp. 1193-1198 ◽  
Author(s):  
Bin Deng ◽  
Nengsheng Ye ◽  
Guoan Luo ◽  
Yiming Wang
Keyword(s):  
Capillary Electrophoresis ◽  
Human Lung ◽  
Lung Carcinomas ◽  
Partial Filling

Bioactive extracts of Ziziphus mauritiana induces apoptosis in A549 human lung epithelial carcinoma cells through the generation of reactive oxygen species

2021 ◽  
Vol 17 ◽  
Author(s):  
Om Prakash ◽  
Shazia Usmani ◽  
Amresh Gupta ◽  
Asif Jafri ◽  
Mohammad Fahad Ullah ◽  
...  
Keyword(s):  
Mtt Assay ◽  
Human Lung ◽  
A549 Cells ◽  
Carcinoma Cells ◽  
Ziziphus Mauritiana ◽  
Meoh Extract ◽  
Lung Carcinomas ◽  
Pharmacological Significance ◽  
Lung Epithelial ◽  
A549 Lung

Background: In recent years, novel metabolites isolated from botanical sources have attracted much attention for traditional and therapeutic significance. The ethnopharmacological studies suggest that Ziziphus mauritiana is a common remedy against several kinds of ailments. Objective: The current study has evaluated the MeOH extract of Ziziphus mauritiana leaves (ZME) through physicochemical, phytochemical, and chromatographic fingerprinting analysis, which displayed an array of biometabolites of pharmacological significance including flavonoids. Methods: The extract was further examined for anticancer activities which revealed promising anticancer properties against human lung epithelial carcinoma cells (A549) and induction of apoptosis impart by ROS. The oxidative stress was evaluated in terms of production and accumulation of cytosolic extent of ROS whereas anticancer perspective was determined by MTT assay, cell morphology analysis, followed by nuclear condensation for the examination of apoptosis induction. Results: Finding suggests that the MeOH extract of ZME markedly exhibited promising anticancer activity against the A549 lung epithelial carcinoma cell. The ZME was found to be most active in the MTT assay against A549 cells while it was less toxic to normal cells. The intracellular ROS generation was remarkably induced by ZME, which correlated with the ability of the flavonoid-rich fractions in the MeOH extract to inhibit cell growth and might induce apoptosis. Conclusion: The present study provides useful insight concerning the promising anticancer potential of ZME against A549 lung carcinomas. However, the clinical correlation will be required for its authorization and in the discovery of significant and least noxious novel agents against lung carcinomas.

scholarly journals Intracellular pH, esterase activity, and DNA measurements of human lung carcinomas by flow cytometry

Cytometry ◽  
1990 ◽  
Vol 11 (3) ◽  
pp. 341-348 ◽  
Author(s):  
F. Liewald ◽  
N. Demmel ◽  
R. Wirsching ◽  
H. Kahle ◽  
G. Valet
Keyword(s):  
Flow Cytometry ◽  
Intracellular Ph ◽  
Esterase Activity ◽  
Human Lung ◽  
Lung Carcinomas ◽  
Dna Measurements

Lectin affinity capillary electrophoresis in glycoform analysis applying the partial filling technique

2004 ◽  
Vol 809 (2) ◽  
pp. 323-329 ◽  
Author(s):  
Maria Bergström ◽  
Mikael Nilsson ◽  
Roland Isaksson ◽  
Ingvar Rydén ◽  
Peter Påhlsson ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Affinity Capillary Electrophoresis ◽  
Lectin Affinity ◽  
Partial Filling ◽  
Partial Filling Technique
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