The Functional Relevance of Ecdysone Receptor Gene to Estrogen-Related Receptor Gene in Regulating the Development of Polyrhachis vicina Roger

2016 ◽  
Vol 13 (7) ◽  
pp. 4141-4150
Author(s):  
Gengsi Xi ◽  
Qiongqiong Ying ◽  
Xiaoming Liu ◽  
Min Zhang ◽  
Zhaoming Wei
Keyword(s):  
Receptor Gene ◽  
Ecdysone Receptor ◽  
Functional Relevance ◽  
Polyrhachis Vicina ◽  
Estrogen Related Receptor

Effect of 20-Hydroxyecdysone and S-adenosylmethionine on the ecdysone receptor gene EcR transcriptional activity in housefly Musca domestica L.

Biomics ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 480-491
Author(s):  
Yu. M. Nikonorov ◽  
T.T. Akhmetkireeva ◽  
G.V. Benkovskaya
Keyword(s):  
Dna Methylation ◽  
Musca Domestica ◽  
Transcriptional Activity ◽  
Regulatory Element ◽  
Receptor Gene ◽  
Tissue Type ◽  
Ecdysone Receptor ◽  
Gender And Age ◽  
The Status ◽  
Terminal Site

Steroid hormone 20-hydroxyecdysone (20E) initiates larval molting start and metamorphosis and regulates reproduction. Its basic receptor is heterodimer including proteins EcR and USP. Ecdysone receptor gene EcR coding protein EcR is a key regulatory element of gene circuits cover considerable part of genes, implicated in growth and development as well as in reproduction of progeny and reactions of organisms to unfavorable factors of environment. The source of methyl groups S-adenosylmethionine (SAM) is in use for biosynthesis of juvenile hormone (JH), methylation of histone proteins and DNA. The main aim of our investigation was evaluation of transcriptional activity of housefly Musca domestica ecdysone receptor gene EcR under adding into ration of 20E and SAM in non-lethal concentrations. Experiments were carried out with larvae and adults of housefly from laboratory strains Shgen and Lgen differ in life span of adults. Change of gene EcR transcripts content in common pool of mRNA in the cells of muscles and gonads, as well as DNA methylation level in 5’-terminal site registered by quantitative real time PCR (RT-PCR). The results of our investigations allow us to suggest existence of mechanism for regulating expression of the EcR gene in M. domestica which is sensitive to exogenic20E and heat stress action as well as to presence of SAM in food. Variations in the mRNA quantitative ratios of EcR gene 5’-and 3’-terminal regions depending on tissue type, gender and age support the hypothesis that this gene can encode several isoforms of the protein EcR. The detected changes in the status of DNA methylation in the 5'-terminal region of the gene and fluctuations in the representation of different mRNA sites after SAM processing suggest the involvement of DNA methylation/demethylation processes in the regulation of EcR gene expression in M. domestica.

scholarly journals Effect of ecdysone receptor gene switch ligands on endogenous gene expression in 293 cells

FEBS Journal ◽  
2007 ◽  
Vol 274 (21) ◽  
pp. 5669-5689 ◽  
Author(s):  
Siva K. Panguluri ◽  
Bing Li ◽  
Robert E. Hormann ◽  
Subba R. Palli
Keyword(s):  
Gene Expression ◽  
Receptor Gene ◽  
Ecdysone Receptor ◽  
Endogenous Gene ◽  
293 Cells ◽  
Gene Switch

scholarly journals Functional characterization of ecdysone receptor gene switches in mammalian cells

FEBS Journal ◽  
2006 ◽  
Vol 273 (24) ◽  
pp. 5550-5563 ◽  
Author(s):  
Siva K. Panguluri ◽  
Prasanna Kumar ◽  
Subba R. Palli
Keyword(s):  
Mammalian Cells ◽  
Receptor Gene ◽  
Functional Characterization ◽  
Ecdysone Receptor ◽  
Gene Switches

scholarly journals Ecdysone signaling underlies the pea aphid transgenerational wing polyphenism

2017 ◽  
Vol 114 (6) ◽  
pp. 1419-1423 ◽  
Author(s):  
Neetha Nanoth Vellichirammal ◽  
Purba Gupta ◽  
Tannice A. Hall ◽  
Jennifer A. Brisson
Keyword(s):  
Phenotypic Plasticity ◽  
Molecular Mechanisms ◽  
Receptor Gene ◽  
Expression Patterns ◽  
Pea Aphid ◽  
Laboratory Model ◽  
Causative Role ◽  
Ecdysone Receptor ◽  
Aphid Density ◽  
Ecdysone Signaling

The wing polyphenism of pea aphids is a compelling laboratory model with which to study the molecular mechanisms underlying phenotypic plasticity. In this polyphenism, environmental stressors such as high aphid density cause asexual, viviparous adult female aphids to alter the developmental fate of their embryos from wingless to winged morphs. This polyphenism is transgenerational, in that the pea aphid mother experiences the environmental signals, but it is her offspring that are affected. Previous research suggested that the steroid hormone ecdysone may play a role in this polyphenism. Here, we analyzed ecdysone-related gene expression patterns and found that they were consistent with a down-regulation of the ecdysone pathway being involved in the production of winged offspring. We therefore predicted that reduced ecdysone signaling would result in more winged offspring. Experimental injections of ecdysone or its analog resulted in a decreased production of winged offspring. Conversely, interfering with ecdysone signaling using an ecdysone receptor antagonist or knocking down the ecdysone receptor gene with RNAi resulted in an increased production of winged offspring. Our results are therefore consistent with the idea that ecdysone plays a causative role in the regulation of the proportion of winged offspring produced in response to crowding in this polyphenism. Our results also show that an environmentally regulated maternal hormone can mediate phenotype production in the next generation, as well as provide significant insight into the molecular mechanisms underlying the functioning of transgenerational phenotypic plasticity.

Broad-complex, but not ecdysone receptor, is required for progression of the morphogenetic furrow in the Drosophila eye

Development ◽  
2001 ◽  
Vol 128 (1) ◽  
pp. 1-11 ◽  
Author(s):  
C.A. Brennan ◽  
T.R. Li ◽  
M. Bender ◽  
F. Hsiung ◽  
K. Moses
Keyword(s):  
Zinc Finger ◽  
Receptor Gene ◽  
Ecdysone Receptor ◽  
Morphogenetic Furrow ◽  
Response Gene ◽  
Ecdysteroid Receptor ◽  
Receptor Isoforms ◽  
Eye Disc ◽  
Drosophila Eye ◽  
Broad Complex

The progression of the morphogenetic furrow in the developing Drosophila eye is an early metamorphic, ecdysteroid-dependent event. Although Ecdysone receptor-encoded nuclear receptor isoforms are the only known ecdysteroid receptors, we show that the Ecdysone receptor gene is not required for furrow function. DHR78, which encodes another candidate ecdysteroid receptor, is also not required. In contrast, zinc finger-containing isoforms encoded by the early ecdysone response gene Broad-complex regulate furrow progression and photoreceptor specification. br-encoded Broad-complex subfunctions are required for furrow progression and proper R8 specification, and are antagonized by other subfunctions of Broad-complex. There is a switch from Broad complex Z2 to Z1 zinc-finger isoform expression at the furrow which requires Z2 expression and responds to Hedgehog signals. These results suggest that a novel hormone transduction hierarchy involving an uncharacterized receptor operates in the eye disc.

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