Study on Cell Behavior on Surfaces with Various Nanostructured Modifications

2019 ◽  
Vol 9 (8) ◽  
pp. 1021-1026 ◽  
Author(s):  
Hung-Ta Lin ◽  
Han-Yi Cheng
Keyword(s):  
Finite Element Analysis ◽  
Cell Proliferation ◽  
Finite Element ◽  
Cell Adhesion ◽  
Control Group ◽  
Element Analysis ◽  
Physical Stimulation ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Stimulation Of

Several researchers have developed finite element analysis (FEA) models on dental implants; however, few have used it to investigate the effects of nanosurface treatment on the cell-implant interface. For cell proliferation and differentiation are believed related to the physical stimulations of modification surfaces. In the present study, the cell adhesion affected by mechanical stimulation from substrate was evaluated by culturing the MG-63 osteoblast-like cells on Ti materials with different chemical composition using computer-aided programs. Three types of surfaces were made by mechanically abraded, anodized and anodized following with hydrothermal treatment on Ti substrates. The cell adhesion behavior was promoted because the physical stimulation of specific surface topography provided cells mechanical external forces. The finite element analysis (FEA) was used to investigate the deformations stresses which cells were suffered on these 3 different modifications surfaces. Results indicated that cells in the HTT group were received larger stimulation of deformations and stresses than the control group (P > 0.01). This confirmed that the superior cell proliferation and differentiation resulted from the specific roughly surfaces of HYT surface for implants.

scholarly journals Effect of core materials for core fabrication for dental implants on in-vitro cytocompatibility of MC3T3-E1 cells

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Jung-Hyun Park ◽  
Hyun Lee ◽  
Seen-Young Kang ◽  
Junesun Kim ◽  
Ji-Hwan Kim
Keyword(s):  
Cell Proliferation ◽  
Cell Adhesion ◽  
Dental Implants ◽  
Laser Scanning Microscopy ◽  
High Cell ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Cad Cam ◽  
Materials Used ◽  
Core Materials

Abstract Background Despite the wide use of dental materials for CAD/CAM system in prosthetic treatment, the effect of the materials, which are used as dental implants core fabricated, on cells involved in dental implant osseointegration is uncertain. This study aimed to investigate and compare the effect of single core materials used for dental implants fabricated by the dental prostheses fabrication process and the CAD/CAM milling method on MC3T3-E1 cells. Methods The materials used for prostheses restoration in this experiment were Porcelain Fused Gold (P.F.G), Lithium disilicate glass ceramic (LiSi2), Zirconia (ZrO2), Nickel-Chromium (Ni-Cr) and Cobalt-Chromium (Co-Cr). MC3T3-E1 cells were cultured and used, the cell adhesion and morphology were observed and analyzed using confocal laser scanning microscopy (CLSM). Methoxyphenyl tetrazolium salt (MTS) and alkaline phosphatase (ALP) assay were used to observe the cell proliferation and differentiation. Results CLSM revealed irregular cell adhesion and morphology and the filopodia did not spread in the Ni-Cr specimen group. Significantly high cell proliferation was observed in the ZrO2 specimen group. The LiSi2 specimen group presented significantly high cell differentiation. Intergroup comparison of cell proliferation and differentiation between the Ni-Cr specimen group and all other specimen groups showed significant differences (p < .05). Conclusion Cell proliferation and differentiation were observed from the cores, which were fabricated with all specimen groups on cytocompatibility except the Ni-Cr specimen group.

scholarly journals 343 Wnt/β-catenin pathway is required for porcine satellite cell proliferation and differentiation to promote skeletal muscle growth

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 97-97
Author(s):  
Zong-ming Zhang ◽  
Chun-qi Gao ◽  
Hui-chao Yan ◽  
Xiu-qi Wang
Keyword(s):  
Skeletal Muscle ◽  
Cell Proliferation ◽  
Satellite Cell ◽  
Muscle Growth ◽  
Control Group ◽  
Skeletal Muscle Growth ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Deficiency Group

Abstract Wnt/β-catenin plays a crucial role in skeletal muscle growth, but its specific mechanism still unclear. In this study, due to the distinct role of lysine in pig industry, we provided it as an entry point to investigate the role of Wnt/β-catenin in governing skeletal muscle growth. Firstly, total 18 weaned piglets were divided into three groups: control group, lysine deficiency group and lysine re-supplementation group (lysine levels added from 0.83% to 1.31% at 14 d). After 28 d experiment, all pigs were slaughtered to measure the change of Wnt/β-catenin in skeletal muscle. Secondly, satellite cell (SC) was isolated and cultured with Wnt activator, such as Wnt3a and WRN (Wnt3a, R-spondin1, Noggin) after lysine deficiency for 48 h to investigate cell proliferation and differentiation ability and the level of Wnt/β-catenin in different conditions. The results showed that compared with the control group, lysine deficiency significantly reduced longissimus dorsi muscle weight and Pax7 positive SC, and inhibited Wnt/β-catenin (P &lt; 0.05). Fortunately, these restrictions were rescued to the control levels by lysine re-supplementation (P &gt; 0.05). Meanwhile, compared with the lysine deficiency group, the MTT and western blotting assay showed cell proliferation ability was significantly increased with re-activated Wnt/β-catenin by re-supplemented lysine, Wnt3a or WRN (P &lt; 0.05), respectively. Moreover, under the condition of cell differentiation, compared with the control group, cell fusion index was significantly decreased in the lysine deficiency group (P &lt; 0.05), whereas it was significantly increased with lysine re-supplementation group, Wnt3a or WRN respective supplementation group in comparison with the lysine deficiency group (P &lt; 0.05). In addition, compared with the lysine deficiency group, the protein levels of myogenic regulatory factors and Wnt/β-catenin pathway were also re-activated by re-supplemented lysine, Wnt3a or WRN (P &lt; 0.05). Collectively, we found Wnt/β-catenin activation is required for porcine SC proliferation and differentiation to promote skeletal muscle growth.

Finite element analysis and animal test verification of nitinol alloy iliac vein stent performance

2019 ◽  
Vol 233 (17) ◽  
pp. 6197-6208 ◽  
Author(s):  
HQ Feng ◽  
SB Wang ◽  
YG Wang ◽  
XQ Li ◽  
YJ Mao ◽  
...  
Keyword(s):  
Finite Element Analysis ◽  
Finite Element ◽  
Fatigue Strength ◽  
Equivalent Stress ◽  
Iliac Vein ◽  
Control Group ◽  
Element Analysis ◽  
Animal Test ◽  
Significant Difference ◽  
Test Verification

The design and fatigue strength of a nitinol iliac vein stent (NIVS) proposed in this study are assessed using the finite element analysis method. The influence of stent diameter and different release scales on its strength, lifespan and biomechanical properties of the vein wall is analyzed for self-developed NIVS of three different diameters (12, 14 and 16mm) and length of 26mm, which were implanted into the corresponding iliac vein with different release scales (80% and 90%). The results obtained strongly indicate that with an increase in the release scale, the equivalent elastic strain, safety factors of fatigue strength, and equivalent stress of the vessel wall exhibit a downward trend, while the most stressed cross-section coincides with the arc of stent-connecting rods. The efficiency and safety of the above stents were verified by in vivo tests (with observation periods of 30, 90 and 180 days after operation) on ten lab pigs, which underwent NIVS implantation into the iliac veins, with observation of hemodynamics, stent deployment, presence/absence of thrombosis, and monitoring of stent lumen loss and its rate. During the animal test verification, the stent release was satisfactory, while the radiography revealed no obvious stent displacement at different time points. In addition, the patency rate of the stent was 100%. Except for the control group, where a small amount of old thrombus was found, other stents exhibited no thrombus; at the same time, there was no significant difference in the lumen loss rate of the stent at each time point. Therefore, the proposed nitinol NIVS samples demonstrated a good performance, accurate positioning, and release, in addition to the reduced risk of lateral iliac vein thrombosis.

scholarly journals Modulation of Tumor Cell Survival, Proliferation, and Differentiation by the Peptide Derived from Tenascin-C: Implication ofβ1-Integrin Activation

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Takuya Iyoda ◽  
Fumio Fukai
Keyword(s):  
Cell Proliferation ◽  
Cell Adhesion ◽  
Cell Survival ◽  
Apoptotic Cell ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
New Strategy ◽  
Cellular Context ◽  
Functional Peptides ◽  
And Migration

Cell adhesion to extracellular matrix (ECM) participates in various biological processes, such as cell survival, proliferation, differentiation, and migration. Since these processes are essential for keeping homeostasis, aberration of these processes leads to a variety of diseases including cancer. Previously, we found that a peptide derived from tenascin- (TN-) C, termed TNIIIA2, stimulates cell adhesion to ECM through activation ofβ1-integrin. It has been shown that TNIIIA2 can modulate cell proliferation and differentiation. Interestingly, TNIIIA2 could not only enhance cell proliferation but also induce apoptotic cell death, depending on cellular context. In this review, we show the function of the peptide TNIIIA2 in cell survival, proliferation, and differentiation and refer to the possibility of new strategy for tumor suppression by regulating cell adhesion status using the ECM-derived functional peptides.

Finite Element Analysis of Stress in Dental Bridge with Implant

2020 ◽  
Vol 10 (6) ◽  
pp. 743-748
Author(s):  
Wan-Ting Huang ◽  
Han-Yi Cheng
Keyword(s):  
Finite Element Analysis ◽  
Finite Element ◽  
Dental Implants ◽  
Dental Implant ◽  
Computer Tomography ◽  
Effective Means ◽  
Control Group ◽  
Finite Element Models ◽  
Element Analysis

The objective of this research was to investigate dental bridges with and without implants. Threedimensional (3D) mandible models were reconstructed by computer tomography (CT) to simulate biting behaviors. The dental implant is an important factor in dental bridge applications. Several studies have investigated finite element models for dental implants; however, few have examined a model for dental bridge with implant. The results revealed that stress was significantly increased when dental bridge was used with implant. Moreover, the dental bridge with implant group demonstrated a relatively big stress in mandible, which was 4.01% lower compared with that of the control group. Dental bridge would be an effective means of recovering dental performance. However, the present research stated that the implant of dental bridge has a potential to increase abnormal stress, and uniformly distributing stress in the dental bridges.

scholarly journals Effect of core materials for core fabrication for dental Implants on in-vitro cytocompatibility of MC3T3-E1 cells

2019 ◽  
Author(s):  
Jung-Hyun Park ◽  
Hyun Lee ◽  
Seen-Young Kang ◽  
Junesun Kim ◽  
Ji-Hwan Kim
Keyword(s):  
Cell Proliferation ◽  
Cell Adhesion ◽  
Dental Implants ◽  
Laser Scanning Microscopy ◽  
High Cell ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Cad Cam ◽  
Materials Used ◽  
Core Materials

Abstract Background: Despite the wide use of dental materials for CAD/CAM system in prosthetic treatment, the effect of the materials, which are used as dental implants core fabricated, on cells involved in dental implant osseointegration is uncertain. This study aimed to investigate and compare the effect of single core materials used for dental implants fabricated by the dental prostheses fabrication process and the CAD/CAM milling method on MC3T3-E1 cells. Methods: The materials used for prostheses restoration in this experiment were Porcelain Fused Gold (P.F.G), Lithium disilicate glass ceramic (LiSi 2 ), Zirconia (ZrO 2 ), Nickel-Chromium (Ni-Cr) and Cobalt-Chromium (Co-Cr). MC3T3-E1 cells were cultured and used, the cell adhesion and morphology were observed and analyzed using confocal laser scanning microscopy (CLSM). Methoxyphenyl tetrazolium salt (MTS) and alkaline phosphatase (ALP) assay were used to observe the cell proliferation and differentiation. Results: CLSM revealed irregular cell adhesion and morphology and the filopodia did not spread in the Ni-Cr specimen group. Significantly high cell proliferation was observed in the ZrO 2 specimen group. The LiSi 2 specimen group presented significantly high cell differentiation. Intergroup comparison of cell proliferation and differentiation between the Ni-Cr specimen group and all other specimen groups showed significant differences (p < .05). Conclusion: Cell proliferation and differentiation were observed from the cores, which were fabricated with all specimen groups on cytocompatibility except the Ni-Cr specimen group.

Application of a Personalized Finite Element Analysis and 3D-Printed Navigation Template in The Treatment of Femoral Neck Fracture With Cannulated Screw

2021 ◽  
Author(s):  
Sizhe Wang ◽  
Bin Wang ◽  
Xiaoquan Lan ◽  
Zhenzhen Xu ◽  
Haoran Huang ◽  
...  
Keyword(s):  
Finite Element Analysis ◽  
Finite Element ◽  
Femoral Head ◽  
Cannulated Screw ◽  
Control Group ◽  
Study Group ◽  
Element Analysis ◽  
Neck Fracture ◽  
3D Printed ◽  
Navigation Template

Abstract Purpose: To investigate the feasibility and accuracy of combining a personalized finite element analysis with 3D-printed navigation template on the treatment of femoral neck fracture (FNF) with cannulated screw. Methods: A total of 60 patients as unstable FNT with cannulated screw were evolved in this study from October 2016 to December 2019, who were randomly divided into two groups (n=30/group): The subjects in the study group were examined using the a finite element analysis according to the three-dimensional CT of hip joint before operation and then underwent 3D-printed navigation template of the femur to complete the implantation of the cannulated screw whereas the other 30 patients in the control group were underwent the implantation of the cannulated screw using the conventional FNF treatment in the inverted isosceles triangle. The success rate of one-time implantation of the cannulated screw, the postoperative shortest distance of talus cortex, and the healing of fracture, necrosis of femoral head and Harris function scores of hip joint in 12 months after operation were recorded and compared between the study and control groups.Results: According to the finite element analysis, the biomechanics of three screws were the most stable when they were close to the bone cortex (<3mm). Further more, it was demonstrated that the patients in the study group have more effectively success rate of one-time nail placement (93.33%) and significant reduction in the distance of talus cortex of cannulated screws (3.04 ± 0.39mm) than those in the control group (66.67% and 5.38±0.71mm). At 12 months post-surgery, higher healing rate of fracture (93.33%) and Harris functional score of hip (93.67 ± 4.01), as well as lower necrosis rate of femoral head (6.67%) were underwent in the study group when compared with the control group (83.33%, 91.57±4.18 and 16.67%). Conclusion: The results of this study suggest that combined application of a personalized finite element analysis and 3D-printed navigation template in the treatment of femoral neck fracture with cannulated screw can not only improve the effective nail placement, but also make the screw more in line with the requirements of biomechanical stability to promote the fracture healing and reduce the risk of femoral head necrosis. So it is a digital orthopedic technology for clinical popularization.

scholarly journals Biomechanical evaluation and finite element analysis of axial-loading simulated experiment of wrist fracture

2020 ◽  
Author(s):  
Yuan-Wei Zhang ◽  
Liang-Yu Xiong ◽  
Zu-Tai Huang ◽  
Xin Xiao ◽  
Su-Li Zhang ◽  
...  
Keyword(s):  
Finite Element Analysis ◽  
Finite Element ◽  
Element Model ◽  
Wrist Fracture ◽  
Biomechanical Analysis ◽  
Control Group ◽  
Element Analysis ◽  
Obvious Effect ◽  
Physiological Load ◽  
Experimental Group

Abstract Objectives This study combined mechanical experiments and finite element analysis (FEA), and verified each other, to assess the biomechanical analysis and effect of wrist fracture, providing theoretical basis for the simulation experiments of wrist fracture and optimal design of wrist protector. Methods and Materials Six cadaveric wrists were included to create experimental specimens. After grouping, the wrist models were axially loaded under physiological load of 600 N, the stress magnitude and distribution of experimental group and control group were obtained. Moreover, a three-dimensional (3D) wrist finite element model (FEM) of a healthy volunteer was developed to verify the rationality and effectiveness of wrist models. Results Within the range of physiological load, the stress of radioulnar palmar unit was high and in shape of pressure, while the stress of radioulnar dorsal unit was relatively lower and in shape of tension. The stresses of radial distal palmar, ulnar distal palmar, radial distal dorsal, ulnar distal dorsal, radial proximal palmar and ulnar proximal palmar units in experimental group were less than those in control group. However, the stresses of radial proximal dorsal and ulnar proximal dorsal units were higher than those in control group. Conclusions Under physiological load, wearing wrist protector can apparently reduce the stress on radioulnar distal palmar, radioulnar proximal palmar and radioulnar distal dorsal units, while has no obvious effect on radioulnar proximal dorsal units. During the process of designing and improving the wrist protector, it is reasonable to place the stress center on radioulnar distal palmar and dorsal units.

scholarly journals Biomechanical Evaluation of Reconstruction of Calcar Femorale Structure for Femoral Neck Fractures: A Comparative Finite Element Analysis

2021 ◽  
Author(s):  
Zhi-Hao Gao ◽  
Jian-Xiong Ma ◽  
Ying Wang ◽  
Bin Lu ◽  
Hao-Hao Bai ◽  
...  
Keyword(s):  
Finite Element Analysis ◽  
Finite Element ◽  
Femoral Neck ◽  
Internal Fixation ◽  
Femoral Neck Fracture ◽  
Biomechanical Properties ◽  
Control Group ◽  
Element Analysis ◽  
Neck Fracture ◽  
Reconstruction Nail

Abstract BackgroundThe optimum internal fixation approach for femoral neck fracture therapy is still debated, and there are few studies on calcar femorale repair following femoral neck fracture. The biomechanical properties of various internal fixation systems can be adequately studied using the finite element method. The goal of this work was to use finite element analysis to examine the biomechanical parameters of Pauwels type III femoral neck fractures under various calcar femorale repair nailing procedures.MethodThe finite element approach was used to numerically investigate the calcar femorale reconstruction method for femoral neck fracture repair. A control group with standard inverted triangle nail placement and internal fixation models with calcar femorale reconstruction nails of 150, 155, and 160 degrees, respectively, were constructed. The four groups of models' von Mises stress and displacement distributions are evaluated and compared.ResultsIn the control group and three calcar femorale reconstruction nail angle models, the maximum stress of femur was (MPa): 106.605, 87.317, 147.691, and 102.072 respectively; The peak stress of four kinds of internal fixation were (MPa): 315.121, 228.819, 198.173 and 208.798. In the control group and three calcar femorale reconstruction nail angle models, the maximum displacement of the femur was (mm): 13.19, 13.183, 12.443, and 12.896 respectively; The displacement peaks of the four internal fixations were (mm): 12.646, 12.625, 11.932 and 12.347.ConclusionsAccording to the results of finite element analysis, the biomechanical performance of calcar femorale reconstruction nail with 150 ° is excellent, which is helpful to the reconstruction of calcar femorale structure. This nail placement method can achieve better biomechanical properties. The results of this study can provide a theoretical reference for clinical practice.

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