Mesoporous Bioactive Glass Promoted Osteoblast Proliferation and Differentiation In Vitro

2019 ◽  
Vol 9 (4) ◽  
pp. 462-467
Author(s):  
Jianwei Chen ◽  
Xiaosheng Yu ◽  
Hao Ji ◽  
Zhen Zong ◽  
Wei Hong ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Osteoblast Proliferation ◽  
Proliferation And Differentiation ◽  
Mesoporous Bioactive Glass

Strontium-incorporated mesoporous bioactive glass scaffolds stimulating in vitro proliferation and differentiation of bone marrow stromal cells and in vivo regeneration of osteoporotic bone defects

2013 ◽  
Vol 1 (41) ◽  
pp. 5711-5722 ◽  
Author(s):  
Yufeng Zhang ◽  
Lingfei Wei ◽  
Jiang Chang ◽  
Richard J. Miron ◽  
Bin Shi ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Bone Defects ◽  
Osteoporotic Bone ◽  
In Vitro Proliferation ◽  
Proliferation And Differentiation ◽  
Mesoporous Bioactive Glass

Sr-containing mesoporous bioactive glass scaffolds significantly enhanced the regeneration of osteoporotic bone defects.

scholarly journals Synthesis and Characterization of Silver–Strontium (Ag-Sr)-Doped Mesoporous Bioactive Glass Nanoparticles

Gels ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 34
Author(s):  
Shaher Bano ◽  
Memoona Akhtar ◽  
Muhammad Yasir ◽  
Muhammad Salman Maqbool ◽  
Akbar Niaz ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Composite Coatings ◽  
Antibacterial Properties ◽  
Bone Diseases ◽  
Osteogenic Potential ◽  
Diffusion Method ◽  
Charge Composition ◽  
In Vitro Bioactivity ◽  
Mesoporous Bioactive Glass

Biomedical implants are the need of this era due to the increase in number of accidents and follow-up surgeries. Different types of bone diseases such as osteoarthritis, osteomalacia, bone cancer, etc., are increasing globally. Mesoporous bioactive glass nanoparticles (MBGNs) are used in biomedical devices due to their osteointegration and bioactive properties. In this study, silver (Ag)- and strontium (Sr)-doped mesoporous bioactive glass nanoparticles (Ag-Sr MBGNs) were prepared by a modified Stöber process. In this method, Ag+ and Sr2+ were co-substituted in pure MBGNs to harvest the antibacterial properties of Ag ions, as well as pro-osteogenic potential of Sr2 ions. The effect of the two-ion concentration on morphology, surface charge, composition, antibacterial ability, and in-vitro bioactivity was studied. Scanning electron microscopy (SEM), X-Ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR) confirmed the doping of Sr and Ag in MBGNs. SEM and EDX analysis confirmed the spherical morphology and typical composition of MBGNs, respectively. The Ag-Sr MBGNs showed a strong antibacterial effect against Staphylococcus carnosus and Escherichia coli bacteria determined via turbidity and disc diffusion method. Moreover, the synthesized Ag-Sr MBGNs develop apatite-like crystals upon immersion in simulated body fluid (SBF), which suggested that the addition of Sr improved in vitro bioactivity. The Ag-Sr MBGNs synthesized in this study can be used for the preparation of scaffolds or as a filler material in the composite coatings for bone tissue engineering.

Europium-Containing Mesoporous Bioactive Glass Scaffolds for Stimulating in Vitro and in Vivo Osteogenesis

2016 ◽  
Vol 8 (18) ◽  
pp. 11342-11354 ◽  
Author(s):  
Chengtie Wu ◽  
Lunguo Xia ◽  
Pingping Han ◽  
Lixia Mao ◽  
Jiacheng Wang ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Mesoporous Bioactive Glass

The Effects of Exercise Serum From Prepubertal Girls and Women on In Vitro Myoblast and Osteoblast Proliferation and Differentiation

2021 ◽  
Vol 33 (2) ◽  
pp. 82-89
Author(s):  
Yasmeen Mezil ◽  
Joyce Obeid ◽  
Inna Ushcatz ◽  
Sandeep Raha ◽  
Brian W. Timmons
Keyword(s):  
Moderate Intensity ◽  
Osteoblast Proliferation ◽  
Moderate Intensity Exercise ◽  
Acute Bout ◽  
Myotube Formation ◽  
Proliferation And Differentiation ◽  
Proliferation In Vitro ◽  
Prepubertal Girls ◽  
Time Point

Purpose: In girls and women, the authors studied the effects of an acute bout of low-impact, moderate-intensity exercise serum on myoblast and osteoblast proliferation in vitro. Methods: A total of 12 pre/early pubertal girls (8–10 y old) and 12 women (20–30 y old) cycled at 60% VO2max for 1 hour followed by 1-hour recovery. Blood samples were collected at rest, mid-exercise, end of exercise, mid-recovery, and end of recovery. C2C12 myoblasts and MC3T3E1 osteoblasts were incubated with serum from each time point for 1 hour, then monitored for 24 hours (myoblasts) or 36 hours (osteoblasts) to examine proliferation. Cells were also monitored for 6 days (myoblasts) to examine myotube formation and 21 days (osteoblasts) to examine mineralization. Results: Exercise did not affect myoblast or osteoblast proliferation. Girls exhibited lower cell proliferation relative to women at end of exercise (osteoblasts, P = .041; myoblasts, P = .029) and mid-recovery (osteoblasts, P = .010). Mineralization was lower at end of recovery relative to rest (P = .014) in both girls and women. Myotube formation was not affected by exercise or group. Conclusion: The systemic environment following one acute bout of low-impact moderate-intensity exercise in girls and women does not elicit osteoblast or myoblast activity in vitro. Differences in myoblast and osteoblast proliferation between girls and women may be influenced by maturation.

Biomimetic Mineralization and Bioactivity of Phosphorylated Chitosan

2005 ◽  
Vol 288-289 ◽  
pp. 429-432 ◽  
Author(s):  
Zhi Qing Chen ◽  
Quan Li Li ◽  
Quan Zen ◽  
Gang Li ◽  
Hao Bin Jiang ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Crystal Nucleation ◽  
Osteoblast Proliferation ◽  
Biomimetic Mineralization ◽  
P Content ◽  
Proliferation And Differentiation ◽  
Nucleation Growth

Phosphorylated chitosans were synthesized as templates to manipulate hydroxyapatite (HA) crystal nucleation, growth and microstructure. Two kinds of insoluble phosphorylated chitosan were soaked in saturated Ca(OH)2 solution for 4 d and in 1.5× SBF (simulated body fluid) solutions for 14 d at 37 °C for biomimetic mineralization. A lower [P]-content of phosphorylated chitosan promoted greater mineralization than higher [P]-content. Phosphorylated chitosan inhibited osteoblast proliferation and differentiation in vitro, while calcium phosphate phosphorylated chitosan composites did not.

Effect of Titanium Nanoparticles on Osteoblast Proliferation

2020 ◽  
Vol 12 (4) ◽  
pp. 455-460
Author(s):  
Yuan Wu ◽  
Cuizhong Liu ◽  
Ming Gao ◽  
Qiang Liang ◽  
Yu Jiang
Keyword(s):  
Histochemical Staining ◽  
Control Group ◽  
Osteoblast Proliferation ◽  
Osteogenic Activity ◽  
Diphenyltetrazolium Bromide ◽  
Proliferation And Differentiation ◽  
Titanium Nanoparticles

This study aimed to observe the effect of titanium nanomaterials on osteoblastsin vitro. Osteoblasts were identified using histochemical staining, and they were examined using an MTT (3-(4,5Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay to determine the proliferation and differentiation of osteoblasts. In addition, we observed the effect of titanium nanomaterials on the function of osteoblasts. Compared with the control group, titanium nanomaterials promoted the growth, proliferation, and differentiation of osteoblasts. Our findings showed that titanium nanomaterials can significantly promote the proliferation of osteoblasts and enhance their osteogenic activity.

Ribavirin, but Not Interferon α-2b, is Associated with Impaired Osteoblast Proliferation and Differentiation In Vitro

2004 ◽  
Vol 75 (2) ◽  
pp. 160-168 ◽  
Author(s):  
R. O. Moreira ◽  
A. Balduíno ◽  
H. S. L. H. Martins ◽  
J. S. N. Reis ◽  
M. E. L. Duarte ◽  
...  
Keyword(s):  
Interferon Α ◽  
Osteoblast Proliferation ◽  
Proliferation And Differentiation

The Primary Investigation on Biological Effect of Mesoporous Bioactive Glass In Vivo

2013 ◽  
Vol 750-752 ◽  
pp. 1651-1655
Author(s):  
Bai Yan Sui ◽  
Cheng Tie Wu ◽  
Jiao Sun
Keyword(s):  
Bioactive Glass ◽  
Biological Effect ◽  
Degradation Products ◽  
Long Term Effect ◽  
Liver And Kidney ◽  
Mesoporous Bioactive Glass

Mesoporous bioactive glass (MBG) has superior bioactivity and degradation than non-mesoporous bioactive glass (BG) in vitro. But the biological effect of MBG in vivo is still unknown. In this study, MBG powders with 20μm were implanted into the femoral condyles in SD rats. BG powders with 20μm were used as a control. The local degradation and osteogenesis were observed at 1 week and 4 weeks after implantation, and the systemic toxicity of the degradation products were also evaluated simultaneously. The results revealed MBG powders had the faster rate of degradation and better osteogenesis effect than BG powders at 4 weeks, although the most of material still remained in situ. Histopathological analyses indicated the degradation products did not have any damage to major organs such as liver and kidney. In conclusion, this preliminary study demonstrated that MBG powders have more excellent biological effect at 4 weeks than that of BG in vivo. However the long-term effect needs to be confirmed.

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