In Vitro Calcium Pretreatment Enhances Bone Formation of Human Adipose Derived Stromal Cells In Vivo

2016 ◽  
Vol 6 (1) ◽  
pp. 20-26
Author(s):  
Shaowen Cheng ◽  
Jingkai Zhao ◽  
Xu Zhang ◽  
Hua Zhang ◽  
Lei Peng ◽  
...  
Keyword(s):  
Bone Formation ◽  
Stromal Cells ◽  
Adipose Derived Stromal Cells

scholarly journals Rat adipose-derived stromal cells expressing BMP4 induce ectopic bone formation in vitro and in vivo

2006 ◽  
Vol 27 (12) ◽  
pp. 1608-1615 ◽  
Author(s):  
Lin LIN ◽  
Xin FU ◽  
Xin ZHANG ◽  
Lian-xu CHEN ◽  
Ji-ying ZHANG ◽  
...  
Keyword(s):  
Bone Formation ◽  
Stromal Cells ◽  
Ectopic Bone Formation ◽  
Adipose Derived Stromal Cells ◽  
Ectopic Bone

In Vivo Bone Formation Following Transplantation of Human Adipose–Derived Stromal Cells That Are Not Differentiated Osteogenically

2008 ◽  
Vol 14 (8) ◽  
pp. 1285-1294 ◽  
Author(s):  
Oju Jeon ◽  
Jong Won Rhie ◽  
Il-Kuen Kwon ◽  
Jae-Hwan Kim ◽  
Byung-Soo Kim ◽  
...  
Keyword(s):  
Bone Formation ◽  
Stromal Cells ◽  
Adipose Derived Stromal Cells ◽  
In Vivo Bone Formation

scholarly journals Bioreactor Processed Stromal Cell Seeding and Cultivation on Decellularized Pericardium Patches for Cardiovascular Use

2020 ◽  
Vol 10 (16) ◽  
pp. 5473
Author(s):  
Roman Matějka ◽  
Miroslav Koňařík ◽  
Jana Štěpanovská ◽  
Jan Lipenský ◽  
Jaroslav Chlupáč ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Stromal Cells ◽  
Subcutaneous Fat ◽  
Adipose Derived Stromal Cells ◽  
Pulsatile Pressure ◽  
Dynamic Cultivation ◽  
Decellularized Tissue ◽  
Static Conditions

(1) Background: Decellularized xenogeneic tissues are promising matrices for developing tissue-engineered cardiovascular grafts. In vitro recellularization of these tissues with stromal cells can provide a better in vivo remodelling and a lower thrombogenicity of the graft. The process of recellularization can be accelerated using a cultivation bioreactor simulating physiological conditions and stimuli. (2) Methods: Porcine pericardium was decellularized using a custom-built decellularization system with an optimized protocol. Autologous porcine adipose-derived stromal cells (PrASCs), isolated from the subcutaneous fat tissue, were used for recellularizing the decellularized pericardium. A custom cultivation bioreactor allowing the fixing of the decellularized tissue into a special cultivation chamber was created. The bioreactor maintained micro-perfusion and pulsatile pressure stimulation in order to promote the ingrowth of PrASCs inside the tissue and their differentiation. (3) Results: The dynamic cultivation promoted the ingrowth of cells into the decellularized tissue. Under static conditions, the cells penetrated only to the depth of 50 µm, whereas under dynamic conditions, the tissue was colonized up to 250 µm. The dynamic cultivation also supported the cell differentiation towards smooth muscle cells (SMCs). In order to ensure homogeneous cell colonization of the decellularized matrices, the bioreactor was designed to allow seeding of the cells from both sides of the tissue prior to the stimulation. In this case, the decellularized tissue was recolonized with cells within 5 days of dynamic cultivation. (4) Conclusions: Our newly designed dynamic bioreactor markedly accelerated the colonization of decellularized pericardium with ASCs and cell differentiation towards the SMC phenotype.

Adipose-Derived Stromal Cells (ADSCs) Promote Bone Formation In Vivo Not by Transforming to Osteoblasts

2018 ◽  
Vol 8 (5) ◽  
pp. 624-631
Author(s):  
Xiaoyu Li ◽  
Jinfeng Yao ◽  
Xinya Du ◽  
Wei Jing ◽  
Weidong Tian
Keyword(s):  
Bone Formation ◽  
Stromal Cells ◽  
Adipose Derived Stromal Cells

Effects of in vitro chondrogenic priming time of bone-marrow-derived mesenchymal stromal cells on in vivo endochondral bone formation

2015 ◽  
Vol 13 ◽  
pp. 254-265 ◽  
Author(s):  
Wanxun Yang ◽  
Sanne K. Both ◽  
Gerjo J.V.M. van Osch ◽  
Yining Wang ◽  
John A. Jansen ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Formation ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Endochondral Bone Formation ◽  
Endochondral Bone

Implications of adipose-derived stromal cells in a 3D culture system for osteogenic differentiation: an in vitro and in vivo investigation

2013 ◽  
Vol 13 (1) ◽  
pp. 32-43 ◽  
Author(s):  
Francis H. Shen ◽  
Brian C. Werner ◽  
Haixiang Liang ◽  
Hulan Shang ◽  
Ning Yang ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Stromal Cells ◽  
Culture System ◽  
3D Culture ◽  
Adipose Derived Stromal Cells ◽  
3D Culture System

Human Adipose-Derived Stromal Cells Efficiently Support Hematopoiesis In Vitro and In Vivo: A Key Step for Therapeutic Studies

2011 ◽  
Vol 20 (12) ◽  
pp. 2127-2138 ◽  
Author(s):  
Fabienne De Toni ◽  
Sandrine Poglio ◽  
Aissa Ben Youcef ◽  
Béatrice Cousin ◽  
Françoise Pflumio ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Adipose Derived Stromal Cells

scholarly journals Hypoxic culture and in vivo inflammatory environments affect the assumption of pericyte characteristics by human adipose and bone marrow progenitor cells

2011 ◽  
Vol 301 (6) ◽  
pp. C1378-C1388 ◽  
Author(s):  
Peter J. Amos ◽  
Carolyn L. Mulvey ◽  
Scott A. Seaman ◽  
Joseph Walpole ◽  
Katherine E. Degen ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Stromal Cells ◽  
Cell Types ◽  
Angiogenic Growth Factors ◽  
Adipose Derived Stromal Cells ◽  
Angiogenic Proteins ◽  
First Time

Previous studies have shown that exposure to a hypoxic in vitro environment increases the secretion of pro-angiogenic growth factors by human adipose-derived stromal cells (hASCs) [Cao Y, et al., Biochem Biophys Res Commun 332: 370–379, 2005; Kokai LE, et al., Plast Reconstr Surg 116: 1453–1460, 2005; Park BS, et al., Biomed Res (Tokyo) 31: 27–34, 2010; Rasmussen JG, et al., Cytotherapy 13: 318–328, 2010; Rehman J, et al., Circulation 109: 1292–1298, 2004]. Previously, it has been demonstrated that hASCs can differentiate into pericytes and promote microvascular stability and maintenance during angiogenesis in vivo (Amos PJ, et al., Stem Cells 26: 2682–2690, 2008; Traktuev DO, et al., Circ Res 102: 77–85, 2008). In this study, we tested the hypotheses that angiogenic induction can be increased and pericyte differentiation decreased by pretreatment of hASCs with hypoxic culture and that hASCs are similar to human bone marrow-derived stromal cells (hBMSCs) in these regards. Our data confirms previous studies showing that hASCs: 1) secrete pro-angiogenic proteins, which are upregulated following culture in hypoxia, and 2) migrate up gradients of PDGF-BB in vitro, while showing for the first time that a rat mesenteric model of angiogenesis induced by 48/80 increases the propensity of both hASCs and hBMSCs to assume perivascular phenotypes following injection. Moreover, culture of both cell types in hypoxia before injection results in a biphasic vascular length density response in this model of inflammation-induced angiogenesis. The effects of hypoxia and inflammation on the phenotype of adult progenitor cells impacts both the therapeutic and the basic science applications of the cell types, as hypoxia and inflammation are common features of natural and pathological vascular compartments in vivo.

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