scholarly journals Transforming Growth Factor-β1 Effects on Endothelial Monolayer Permeability Involve Focal Adhesion Kinase/Src

2007 ◽  
Vol 37 (4) ◽  
pp. 485-493 ◽  
Author(s):  
Young H. Lee ◽  
Usamah S. Kayyali ◽  
Anne Marie Sousa ◽  
Thomas Rajan ◽  
Robert J. Lechleider ◽  
...  
Keyword(s):  
Growth Factor ◽  
Focal Adhesion Kinase ◽  
Focal Adhesion ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β1 ◽  
Endothelial Monolayer ◽  
Monolayer Permeability

Rearrangement of adherens junctions by transforming growth factor-β1: role of contraction

1999 ◽  
Vol 276 (4) ◽  
pp. L582-L595 ◽  
Author(s):  
Victor Hurst ◽  
Peter L. Goldberg ◽  
Fred L. Minnear ◽  
Ronald L. Heimark ◽  
Peter A. Vincent
Keyword(s):  
Growth Factor ◽  
Cell Separation ◽  
Myosin Light Chain Kinase ◽  
Myosin Light Chain ◽  
Transforming Growth Factor ◽  
Adherens Junction ◽  
Transforming Growth Factor Β1 ◽  
Cell Periphery ◽  
Endothelial Monolayer ◽  
Tgf Β1

The signal transduction pathways that lead to disruption of pulmonary endothelial monolayer integrity by transforming growth factor-β1 (TGF-β1) have not been elucidated. The purpose of this investigation was to determine whether disassembly of the adherens junction is temporally associated with the TGF-β1-induced decrease in pulmonary endothelial monolayer integrity. Measurement of albumin clearance and electrical resistance showed that monolayer integrity started to decrease between 1 and 2 h post-TGF-β1 treatment and continued to slowly decrease over the next 6 h. Immunofluorescence microscopy of monolayers between 2 and 3 h post-TGF-β1 showed that β-catenin, plakoglobin, α-catenin, and cadherin-5 were colocalized both at the cell periphery and in newly formed bands that are perpendicular to the cell-cell border. At 4 h post-TGF-β1, cells began separating; however, β- and α-catenin, plakoglobin, and cadherin-5 could still be found at the cell periphery at areas of cell separation and in strands between separated cells. By 8 h, these junctional proteins were no longer present at the cell periphery at areas of cell separation. The myosin light chain kinase inhibitor KT-5926 prevented the TGF-β1-induced change in integrity but did not inhibit the formation of actin stress fibers or the formation of bands containing adherens junction proteins that were perpendicular to the cell-cell junction. Overall, these results suggest that adherens junction disassembly occurs after cell separation during TGF-β1-induced decreases in pulmonary endothelial monolayer integrity and that the loss of integrity may be due to the activation of a myosin light chain kinase-dependent signaling cascade.

scholarly journals Transforming Growth Factor-β Stimulates Intestinal Epithelial Focal Adhesion Kinase Synthesis via Smad- and p38-Dependent Mechanisms

2008 ◽  
Vol 173 (2) ◽  
pp. 385-399 ◽  
Author(s):  
Mary F. Walsh ◽  
Dinakar R. Ampasala ◽  
James Hatfield ◽  
Richard Vander Heide ◽  
Silke Suer ◽  
...  
Keyword(s):  
Growth Factor ◽  
Focal Adhesion Kinase ◽  
Focal Adhesion ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β ◽  
Intestinal Epithelial
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