scholarly journals Measurement of IL-13–Induced iNOS-Derived Gas Phase Nitric Oxide in Human Bronchial Epithelial Cells

2007 ◽  
Vol 37 (1) ◽  
pp. 97-104 ◽  
Author(s):  
Vinod Suresh ◽  
Justin D. Mih ◽  
Steven C. George
Keyword(s):  
Nitric Oxide ◽  
Epithelial Cells ◽  
Gas Phase ◽  
Bronchial Epithelial Cells ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial

An electrogenic amino acid transporter in the apical membrane of cultured human bronchial epithelial cells

1998 ◽  
Vol 275 (5) ◽  
pp. L917-L923 ◽  
Author(s):  
Luis J. V. Galietta ◽  
Luciana Musante ◽  
Leila Romio ◽  
Ubaldo Caruso ◽  
Annarita Fantasia ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Amino Acid ◽  
Epithelial Cells ◽  
Apical Membrane ◽  
Short Circuit ◽  
Short Circuit Current ◽  
Bronchial Epithelial Cells ◽  
Maximal Effect ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial

We performed Ussing chamber experiments on cultured human bronchial epithelial cells to look for the presence of electrogenic dibasic amino acid transport. Apical but not basolaterall-arginine (10–1,000 μM) increased the short-circuit current. Maximal effect and EC50were ∼3.5 μA/cm2and 80 μM, respectively, in cells from normal subjects and cystic fibrosis patients. The involvement of nitric oxide was ruled out because a nitric oxide synthase inhibitor ( NG-nitro-l-arginine methyl ester) did not decrease the arginine-dependent current. Apicall-lysine,l-alanine, andl-proline, but not aspartic acid, were also effective in increasing the short-circuit current, with EC50values ranging from 26 to 971 μM. Experiments performed with radiolabeled arginine demonstrated the presence of an Na+-dependent concentrative transporter on the apical membrane of bronchial cells. This transporter could be important in vivo to maintain a low amino acid concentration in the fluid covering the airway surface.

scholarly journals Estrogen induces nitric oxide production in human bronchial epithelial cells

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Elizabeth A Townsend ◽  
Michael A Thompson ◽  
Stephen D Cassivi ◽  
Christina M Pabelick ◽  
Y S Prakash
Keyword(s):  
Nitric Oxide ◽  
Epithelial Cells ◽  
Bronchial Epithelial Cells ◽  
Nitric Oxide Production ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial ◽  
Oxide Production

scholarly journals Effects of Nitric Oxide on Pseudomonas aeruginosa Infection of Epithelial Cells from a Human Respiratory Cell Line Derived from a Patient with Cystic Fibrosis

2003 ◽  
Vol 71 (5) ◽  
pp. 2341-2349 ◽  
Author(s):  
Katharine E. A. Darling ◽  
Thomas J. Evans
Keyword(s):  
Nitric Oxide ◽  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Epithelial Cells ◽  
Airway Inflammation ◽  
Bronchial Epithelial Cells ◽  
No Production ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial ◽  
Different Strains

ABSTRACT Cystic fibrosis (CF) is characterized by airway inflammation and chronic bacterial lung infection, most commonly with Pseudomonas aeruginosa, an opportunistic human pathogen. Despite the persistent airway inflammation observed in patients with CF, although phagocyte inducible nitric oxide synthase (iNOS) production is upregulated, expression of iNOS in the respiratory epithelium is markedly reduced. Given the antimicrobial action of NO, this may contribute to the chronic airway infection of this disease. To define the role of epithelium-derived NO in airway defense against P. aeruginosa, we infected differentiated human bronchial epithelial cells derived from a patient with CF (CFBE41o- cells) with different strains of this pathogen at low multiplicities of infection. Using cells transfected with human iNOS cDNA, we studied the effect of NO on P. aeruginosa replication, adherence, and internalization. P. aeruginosa adherence to iNOS-expressing cells was reduced by 44 to 72% (P = 0.02) compared with control values. Absolute P. aeruginosa uptake into these cells was reduced by 44%, but uptake expressed as a percentage of adherent bacteria did not differ from the control uptake. Survival of P. aeruginosa within iNOS-expressing cells was reduced at late times postinfection (P = 0.034). NO production did not alter host cell viability. NO production reduced P. aeruginosa adherence to human bronchial epithelial cells and enhanced killing of internalized bacteria, suggesting that a lack of epithelial iNOS in patients with CF may contribute to P. aeruginosa infection and colonization.

Diprivan attenuates the cytotoxicity of nitric oxide in cultured human bronchial epithelial cells

2002 ◽  
Vol 28 (8) ◽  
pp. 1145-1150 ◽  
Author(s):  
Chun Huang ◽  
Paul N. Slovin ◽  
Richard B. Nielsen ◽  
Jeffrey W. Skimming
Keyword(s):  
Nitric Oxide ◽  
Epithelial Cells ◽  
Bronchial Epithelial Cells ◽  
Human Bronchial Epithelial Cells ◽  
Bronchial Epithelial
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